Aim, design and setting
Study objectives: A randomized controlled trial (RCT) will be performed with the design shown in Fig. 1. The proportion of subjects requiring high-dose opioids (≥60 mg/day of morphine or ≥40 mg/day of oxycodone administered on day 0 will be calculated from use of immediate-release preparations and compared between the morphine and oxycodone groups using the morphine-equivalent dose in patients with the GG or non-GG COMT rs4680 SNP.
Participants
Inclusion criteria are 1) patients with advanced malignant tumors, and 2) non-daily use of opioids. At the second registration, the criteria are 1) cancer pain targeted for daily treatment with opioids, NSAIDs or acetaminophen, 2) NRS ≥3 (average over 24 h), 3) opioid treatment-naive within 30 h, 4) no chemotherapy, radiotherapy, or bisphosphonate administration newly started within 2 weeks, and 5) written informed consent.
The exclusion criteria are 1) patients with chronic renal failure (glomerular filtration rate, 30 mL/min), 2) patients with severe hepatic or respiratory failure, and 3) patients deemed ineligible for the study by the study coordinator or a collaborative investigator (ex. neuropathic pain or predominant spontaneous pain only, and have histories of opioid/drug abuse or alcoholism).
Endpoints
Primary endpoint
The primary endpoint is the proportion of subjects requiring high-dose opioids calculated from use of the immediate-release preparation on day 0 in a parallel group comparison.
Secondary endpoints
The secondary endpoints are the Hospital Anxiety and Depression Scale (HADS) score for anxiety and depression; the European Organization for Research and Treatment of Cancer (EORTC) QLQ-C15-PAL for score for QOL; the Short-Form McGill Pain Questionnaire 2 (SF-MPQ2) score for pain characterization; and the Pain Catastrophizing Scale (PCS) for estimation of impact on pain prognosis. Adverse events (e.g., constipation, somnolence, nausea, pruritus, ischuria) will be evaluated using the Common Terminology Criteria for Adverse Events (CTCAE) ver. 4.0.
Measurement tools
Performance status (PS)
The European Cooperative Oncology Group (ECOG) PS system will be used for evaluation of PS by primary physicians [9].
Numerical rating scale (NRS)
The NRS will be used to evaluate pain for its better validity, sensitivity, and convenience compared to other scales [10] and its widespread use in many clinical studies.
Hospital anxiety and depression scale (HADS)
The HADS will be used for measurement of psychiatric symptoms (anxiety and depression) of patients with a physical disease. HADS is a screening tool that allows assessment based on a small number of items. Its reliability and validity have been verified internationally [11, 12].
European Organization for Research and Treatment of cancer (EORTC) QLQ-C15-PAL
EORTC QLQ-C15-PAL will be used for evaluation of patient QOL. The reliability and validity of the Japanese version have been confirmed [13].
Short-form McGill pain questionnaire 2 (SF-MPQ-2, Japanese version)
The SF-MPQ-2 will be used to examine differences in effects due to pain mechanisms. The reliability and validity of the Japanese version have been verified [14].
Pain catastrophizing scale (PCS)
The severity of cancer-related pain is influenced by engagement of patients in catastrophic thinking, such as "my pain will undoubtedly never improve" [15]. This effect will be measured using the Japanese version of the PCS, for which the validity and reliability have been shown [16].
Common terminology criteria for adverse events (CTCAE)
The worst grade of an adverse event during the preceding period will be assessed using the CTCAE v.4.0, Japanese Clinical Oncology Group (JCOG) version.
Protocol treatment
In this prospective clinical study, cancer patients will undergo initial registration and genotyping for SNPs with a Taqman SNP Genotyping Assay (Life Technologies). In the first application of opioid treatment after occurrence of cancer pain, the patients will be divided into a GG group and a non-GG group based on the COMT rs4680 SNP and then undergo second registration, after which the protocol treatment will be started. Each group will be randomized into subgroups that will receive immediate-release morphine (Tmax about 1 h) and immediate-release oxycodone (Tmax about 2 h), respectively, with doses subjected to titration. Dose titration will be performed to decrease pain by ≥33% on the NRS pain scale, as well as reducing NRS to ≤3. The patients were tested with opioid according to the guideline for titration and following regular dosing (NCCN Guidelines™, Adult Cancer Pain) by specialized palliative care doctors. On this step, they explained potential benefits and adverse effects to their patients. Subsequently, the controlled-release opioid will be administered (Fig. 2). In all subgroups, the incidences of subjects requiring a high opioid dose (as a morphine-equivalent dose), psychological tests, and evaluation of QOL are defined as quantitative clinical endpoints to investigate the efficacies of morphine and oxycodone in the GG and non-GG groups. Candidate biomarkers related to onset of adverse effects of opioids will also be measured to examine correlations in an integrated manner. NRS, psychological tests, QOL evaluation, and blood collection will be performed before opioid treatment and on days 1 and 8 after starting treatment. The screening of biomarkers correlated with opioid adverse effects is performed as a subsidiary study. Randomization will be performed on a web page produced by To Field Inc. using the minimization method with modulating factors of age, sex, performance status (PS), and site of pain.
Completion of treatment and use of other drugs: Treatment is completed 8 days after the beginning of the protocol. As supportive therapy, the opioid dose can be increased or reduced as appropriate, based on the decision of a primary physician. Regarding combination therapy, there is no limitation on the use of any other drug.
Subsidiary biomarker study: This study will include screening for biomarkers correlated with adverse effects of opioids. Therefore, the following items will be examined as potential pharmacological biomarkers: (1) serum chemokine levels, (2) polymorphisms in opioid function-related genes, (3) serum glycan analysis, and (4) psychological tests, QOL scale and others. Treatment will be performed in accordance with normal guidelines and will not be changed for this study.
Sample handling: Blood will be collected at initial registration, before starting opioid treatment after second registration, and on days 1 and 8 after starting treatment. Samples will be collected and stored in the laboratory of the Department of Medical Oncology, Kindai University Faculty of Medicine. Samples will be encoded with identification numbers by a manager of personal information at the time of registration.
Ethical issues: All patients are required to provide written informed consent. The study will be performed in accordance with the Declaration of Helsinki and the International Conference on Harmonization and Good Clinical Practice. The protocol has been approved by the Institutional Review Board at each study site.
Statistical analysis
The null hypothesis is that the proportion of subjects requiring high-dose opioids is equal between the morphine and oxycodone groups for subjects with the GG genotype. This null hypothesis will be evaluated using Fisher’s exact test at the one-tailed significance level of 2.5%. The 95% confidence interval (CI) of the difference in the proportion of subjects requiring high-dose opioids will be calculated as an estimate of the therapeutic efficacy. The proportion of subjects requiring high-dose opioids and the 95% CI will also be calculated in each group.
Sample size calculation: Our preliminary study [2] indicated that high-dose morphine was required by 36.8% of GG genotype subjects, and results for 100 subjects in a preceding study at Kindai University and 160 subjects in the series in this study suggested that this proportion was about 46%. On the basis of previous data and discussions at a conference at Kindai University Hospital, about 5% of these subjects are likely to require high-dose oxycodone. Thus, we assumed that 46% of subjects would require high-dose morphine and 5% would require high-dose oxycodone in the GG group. Under these assumptions, when we set the one-tailed significance level of 2.5% and power of 80%, Fisher’s exact test requires 31 GG subjects in each drug group; that is, 62 subjects with the GG genotype. Since the GG genotype is estimated to account for around 46% of the population, the number of registrations required is 135 subjects. Therefore, the target is defined as 140 subjects to allow for dropout and subjects who cannot be analyzed.