Fig. 7

5-LO-induced ROS mediates the decrease of Akt activity by DHEA and NALA. a SNU-1041 cells were treated with DHEA or NALA (40 μM) plus NAC (1 mM). At 24 h, cells were harvested and applied to western blotting. b Cells were transfected with the indicated siRNA (200 nM). Forty-eight hours later cells were treated with DHEA or NALA (40 μM). After additional 24 h, cells were harvested and applied to western blotting (si-NC was used for control). The membranes were incubated with anti-β-actin plus anti-p-Akt or anti-pan-Akt for 2 h at room temperature (β-actin was used to show an housekeeping gene). Data are presented as a representative of 3 separate experiments