Fig. 4

Aspirin retards migration of A549 cells by restoration of E-cadherin expression. a Phase contrast images depicts migration of A549 cells upon 24 h treatment with aspirin in a dose-dependent manner (left panel) was determined by wound healing assay and percent cell migration was represented graphically (right panel). Scale bar: 100 μm. b Graphical representation of percent cell death in PBMCs upon treatment with aspirin in a dose-dependent manner. c Phase contrast images depicting migration of untreated (upper left panel) and aspirin (2.5 mM)-treated (lower left panel) A549 cells in a time-dependent manner was determined by wound healing assay and percent cell migration was represented graphically (right panel). Scale bar: 100 μm. d Graphical representation of percent cell death in PBMCs upon treatment with 2.5 mM aspirin in a time-dependent manner. e Expression profiles of E-cadherin and Slug mRNA (left panel) and protein (right panel) levels of A549 cells upon treatment with aspirin in a time dependent manner as determined by RT-PCR and western blot analysis, respectively. f Protein expression profiles of migration related markers, Vimentin, Twist, MMP-2 and MMP-9 in untreated and aspirin-treated A549 cells as determined by western blot analysis. α-Actin/ GAPDH served as loading controls. Data are presented as mean ± SEM or representative of three independent experiments