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Fig. 4 | BMC Cancer

Fig. 4

From: Pan-Bcl-2 inhibitor Obatoclax is a potent late stage autophagy inhibitor in colorectal cancer cells independent of canonical autophagy signaling

Fig. 4

Obatoclax blocks autophagy independent of canonical autophagy signaling. a and b Representative western blotting for HT29 cells treated with obatoclax. siRNA mediated knockdown of Atg12 and Atg7 is shown in (a), siRNA mediated knockdown of Mcl-1 or Beclin-1 is shown in (b). c Flow cytometric analysis for apoptosis induction in full supplemented (left) or starving (right) HT29 cells after knockdown of Atg7 or Beclin-1 and treatment with obatoclax. Values are expressed as mean ± SD. Staurosporine 2 μM for 2 h served as a positive control for cell death induction

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