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Table 1 Primary and secondary antibodies

From: Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells

Name 1° /2° Target Dilution Species Commercial availability Ref.
α/9d Tm1, 2, 3, 5a*, 5b*, 6* WB/IF: 1:500 Mouse monoclonal (IgG2b κ; clone 15D12.2 [79]
γ/9d Tm5NM1/2 WB/IF: 1:500 Mouse monoclonal (IgG2b κ; clone 2G10.2) [79]
WD4/9d Tm4 WB/IF: 1:500 Rabbit polyclonal Millipore [80]
CG1 Tm1 IF: 1:50 Mouse monoclonal (IgG) [79]
CG3 Tm5NM1-11 WB/IF: 1:150 Mouse monoclonal (IgM) [79]
CGβ6 Tm2, 3 IF: 1:150 Mouse monoclonal (IgM) [79]
Tm311 Tm1, 2, 3, 6*, Br1*, plus α-,β-, γ-muscle* IF: 1:500 Mouse monoclonal (IgG1), clone TM311 Sigma-Aldrich [81]
C4 Actin Actin WB: 1:5000 Mouse monoclonal, (IgG1κ; clone C4) Millipore [82]
α-actinin α-actinin 1-4 WB: 1:200 Rabbit polyclonal (H-300) Santa Cruz Biotechnology [83]
HIF-1α HIF-1α WB: 1:500 Mouse monoclonal (IgG1; clone 54) BD Biosciences [20]
HIF-2α HIF-2α WB: 1:500 Rabbit polyclonal Novus Biologicals [84]
GAM-HRP Mouse (H + L) WB: 1:5000 Goat polyclonal Bio-Rad [85]
GAR-HRP Rabbit (H + L) WB: 1:5000 Goat polyclonal Bio-Rad [85]
Alexa Fluor® 555 GAM Mouse (H + L) IF: 1:1000 Goat polyclonal Molecular Probes [86]
Alexa Fluor® 488 GAR Rabbit (H + L) IF: 1:1000 Goat polyclonal Molecular Probes [86]
  1. *Tropomyosin isoforms not expressed by SH-EP (Stehn et al., unpublished data). Those antibodies not commercially available were generated in-house
  2. WB Western blot, IF immunofluorescence, primary antibody, secondary antibody, GAM goat anti-mouse, GAR goat anti-rabbit, HRP horseradish peroxidase, H heavy chain of IgG, L light chain of IgG