Table 1 Primary and secondary antibodies
From: Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells
Name | 1° /2° | Target | Dilution | Species | Commercial availability | Ref. |
|---|---|---|---|---|---|---|
α/9d | 1° | Tm1, 2, 3, 5a*, 5b*, 6* | WB/IF: 1:500 | Mouse monoclonal (IgG2b κ; clone 15D12.2 | – | [79] |
γ/9d | 1° | Tm5NM1/2 | WB/IF: 1:500 | Mouse monoclonal (IgG2b κ; clone 2G10.2) | – | [79] |
WD4/9d | 1° | Tm4 | WB/IF: 1:500 | Rabbit polyclonal | Millipore | [80] |
CG1 | 1° | Tm1 | IF: 1:50 | Mouse monoclonal (IgG) | – | [79] |
CG3 | 1° | Tm5NM1-11 | WB/IF: 1:150 | Mouse monoclonal (IgM) | – | [79] |
CGβ6 | 1° | Tm2, 3 | IF: 1:150 | Mouse monoclonal (IgM) | – | [79] |
Tm311 | 1° | Tm1, 2, 3, 6*, Br1*, plus α-,β-, γ-muscle* | IF: 1:500 | Mouse monoclonal (IgG1), clone TM311 | Sigma-Aldrich | [81] |
C4 Actin | 1° | Actin | WB: 1:5000 | Mouse monoclonal, (IgG1κ; clone C4) | Millipore | [82] |
α-actinin | 1° | α-actinin 1-4 | WB: 1:200 | Rabbit polyclonal (H-300) | Santa Cruz Biotechnology | [83] |
HIF-1α | 1° | HIF-1α | WB: 1:500 | Mouse monoclonal (IgG1; clone 54) | BD Biosciences | [20] |
HIF-2α | 1° | HIF-2α | WB: 1:500 | Rabbit polyclonal | Novus Biologicals | [84] |
GAM-HRP | 2° | Mouse (H + L) | WB: 1:5000 | Goat polyclonal | Bio-Rad | [85] |
GAR-HRP | 2° | Rabbit (H + L) | WB: 1:5000 | Goat polyclonal | Bio-Rad | [85] |
Alexa Fluor® 555 GAM | 2° | Mouse (H + L) | IF: 1:1000 | Goat polyclonal | Molecular Probes | [86] |
Alexa Fluor® 488 GAR | 2° | Rabbit (H + L) | IF: 1:1000 | Goat polyclonal | Molecular Probes | [86] |