Fig. 4

Overexpression of miR-449a in Huh7 and LM9 cells reverses epithelial mesenchymal transfer. a Expression of epithelial markers and mesenchymal markers were compared by western blot analysis between miR-control-Huh7, miR-control-LM9 and miR-449a-Huh7, miR-449a-LM9 cells. α-tubulin was used as a loading control. b miR-449a overexpression enhanced the mRNA level of E-cadherin in hepatoma cells. **P < 0.01. c The antagonism of endogenous miR-449a promoted the EMT of hepatoma cells. HepG2 cells were transfected with anti-miR-449a or its control (anti-miR-NC) for 72 h and analyzed by immunoblotting. α-tubulin was used as a loading control. d Immunohistochemistry staining showed a decreased expression of fibronectin and vimentin and an increased expression of E-cadherin and β-catenin in tumour tissues originating from miR-449a-Huh7 cells, compared with that originating from miR-control-Huh7 cells. Scale bar: 50 mm