Fig. 5

IGF1-R protein in Jurkat and Molt-4 clones over-expressing IGFBP7. a: Jurkat clones transfected with pCntrl or pIGFBP7 were incubated for four days without medium exchange. Cells were analyzed by flow cytometry for IGF1-R in viable cells according to their forward and side scatter properties. Representative dot plots show each clone with a respective isotype-matched negative control (light grey) from an exemplary out of five experiments. b: Results from five independent IGF-1R median fluorescence intensity (MFI) determinations in duplicates, lines indicate means and SEM. **P-value ≤ 0.01 (Mann–Whitney-U test) c: Jurkat or Molt-4 clones were cultured for one day and lysates subjected to Western-blot analysis for IGF1-Rβ and β-actin. The cell line HeLa served as positive control. The Image is representative for three independent experiments