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Table 4 Blood methylation levels in 12 CRC cases positive before and after tumour resection

From: Evaluation of an assay for methylated BCAT1 and IKZF1 in plasma for detection of colorectal neoplasia

Case characteristics Proportion of methylated BCAT1 and IKZF11 (% of total yield)
Tumour location Stage Before resection After resection Δ Days
Sigmoid l <0.00001 0 140
Splenic flexure l <0.00001 0 48
Caecum llA 1.8 0 115
Ascending llA 0.7 <0.00001 70
Sigmoid llA 5.6 0 162
Sigmoid llA 2.6 0 64
Ascending llA 1.7 0 83
Ascending llA 1.4 0 39
Caecum lllA 5.0 0 58
Rectum lllA 0.8 0 47
Ascending lllB 0.9 0 50
Sigmoid lllC 0.6 0.6 157
  1. 1The lower limit of the linear range for the qPCR assay was 100pg per reaction. The average DNA amount per reaction was 2 ng, thus methylation levels estimated to be <5% but above zero are extrapolated and most likely inaccurate