Fig. 1
From: Over-expressing Akt in T cells to resist tumor immunosuppression and increase anti-tumor activity
Akt activity of T cells is down-regulated in tumor environment. a CD8+ T cells isolated from OT-1 transgenic mice were stimulated with anti-CD3 and anti-CD28 antibodies for 2 days, and then incubated with or without B16-OVA tumor cell culture supernatant. 24 h later, cells were collected, and the levels of Akt, phosphorylated Akt, and β-actin were examined by western blot. b CD8+ T cells were isolated from tumor draining lymph nodes (TDLN) or distant lymph nodes of B16-OVA tumor bearing mice, the levels of Akt, phosphorylated Akt, and β-actin were examined by western blot