This article has Open Peer Review reports available.
Primary ciliogenesis defects are associated with human astrocytoma/glioblastoma cells
© Moser et al; licensee BioMed Central Ltd. 2009
Received: 7 July 2009
Accepted: 17 December 2009
Published: 17 December 2009
Primary cilia are non-motile sensory cytoplasmic organelles that have been implicated in signal transduction, cell to cell communication, left and right pattern embryonic development, sensation of fluid flow, regulation of calcium levels, mechanosensation, growth factor signaling and cell cycle progression. Defects in the formation and/or function of these structures underlie a variety of human diseases such as Alström, Bardet-Biedl, Joubert, Meckel-Gruber and oral-facial-digital type 1 syndromes. The expression and function of primary cilia in cancer cells has now become a focus of attention but has not been studied in astrocytomas/glioblastomas. To begin to address this issue, we compared the structure and expression of primary cilia in a normal human astrocyte cell line with five human astrocytoma/glioblastoma cell lines.
Cultured normal human astrocytes and five human astrocytoma/glioblastoma cell lines were examined for primary cilia expression and structure using indirect immunofluorescence and electron microscopy. Monospecific antibodies were used to detect primary cilia and map the relationship between the primary cilia region and sites of endocytosis.
We show that expression of primary cilia in normal astrocytes is cell cycle related and the primary cilium extends through the cell within a unique structure which we show to be a site of endocytosis. Importantly, we document that in each of the five astrocytoma/glioblastoma cell lines fully formed primary cilia are either expressed at a very low level, are completely absent or have aberrant forms, due to incomplete ciliogenesis.
The recent discovery of the importance of primary cilia in a variety of cell functions raises the possibility that this structure may have a role in a variety of cancers. Our finding that the formation of the primary cilium is disrupted in cells derived from astrocytoma/glioblastoma tumors provides the first evidence that altered primary cilium expression and function may be part of some malignant phenotypes. Further, we provide the first evidence that ciliogenesis is not an all or none process; rather defects can arrest this process at various points, particularly at the stage subsequent to basal body association with the plasma membrane.
Cilia are microtubule-based organelles that extend from the surface of cells and can be classified into two categories, motile cilia with a 9+2 arrangement of microtubules and non-motile (primary) cilia with 9+0 arrangement of microtubules (reviewed in ). Most vertebrate cells contain a single non-motile primary cilium that is assembled in a step-wise manner from the distal end of a mature centriole at the centrosome. It is now known that the formation and maintenance of a primary cilium is a complex process involving a wide variety of proteins that include members of the intraflagellar transport (IFT) complex [2–5], pericentrin [6, 7], ODF2 [8–10], Cep164 , ALMS1 [12, 13], EB1  and Cep290 [15–17]. In addition, certain proteins involved in cell cycle progression are linked to primary cilium expression (for review see [18–20]).
Primary cilia have been implicated in signal transduction, cell to cell communication, left and right pattern embryonic development, sensation of fluid flow, regulation of calcium levels, mechanosensation and growth factor signaling. Primary cilia have been detected in the central nervous system (CNS) where the deletion of primary cilia in pro-opiomelanocortin hypothalamic neurons resulted in hyperphagia [2, 21]. CNS primary cilia have also been linked to CNS development and the Sonic hedgehog (Shh) signaling pathway [22–25]. Shh signaling components including Patched (Ptc), Smoothened (Smo), Suppressor of fused and Gli transcription factors have been reported to concentrate in CNS primary cilia [26–28]. Astrocytes in the subventricular zone extend a primary cilium into the ventricle suggesting that they may play a role in sensing cerebral spinal fluid (CSF) ion concentration, pH, osmolarity and perhaps changes in protein or glucose levels . Similarly, it is possible that primary cilia in astrocytes may sense levels of neurotransmitters, growth factors, hormones, osmolarity, ions, pH and fluid flow in the extracellular space and relay homeostatic information (or lack thereof) back to the cell body. Diseases associated with faulty primary cilia reinforce the concept that primary cilia are required for the proper development and function of the brain. These diseases include Alström, Bardet-Biedl, Joubert, Meckel-Gruber and oral-facial-digital type 1 syndromes where common neurological related pathologies include obesity, ataxia and mental retardation .
The finding that primary cilia are linked to cell cycle regulation and progression has led to suggestions that they may play a role in tumor formation, a supposition that has been validated by several recent studies [31, 32]. In the present study, we undertook a comparative investigation of primary cilia in cultured primary human astrocytes and compared them to those found in five human astrocytoma/glioblastoma cell lines. We demonstrate that the primary cilium region in cultured astrocyte cells is structurally complex and includes foci for endocytosis-based signaling. This indicates that there is a spatial link between receptor pathways associated with endocytosis and the primary cilium microenvironment. Importantly, we document that in each of the five astrocytoma/glioblastoma cell lines, fully formed primary cilia are either expressed at a very low level, are completely absent or do not proceed through all the stages of ciliogenesis. In addition, we noted several defects affecting the structure of astrocytoma/glioblastoma centrioles that were not observed in primary human astrocytes. We conclude that aberrant ciliogenesis is a common defect found in cells derived from astrocytomas/glioblastomas and this deficiency likely contributes to the phenotype of these malignant cells.
Primary cilia were marked by one of three separate antibodies: rabbit anti-adenylyl cyclase III (ACIII) antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA), rabbit anti-glu tubulin (Chemicon, Temecula, CA), and mouse monoclonal to acetylated tubulin (Sigma-Aldrich, Oakville, ON, Canada). Mouse monoclonals to golgin 97 (CDF4 clone) and TGN38 were from Invitrogen (Burlington, ON, Canada) and Santa Cruz Biotechnology Inc. (Santa Cruz, CA), respectively. Mouse monoclonals to early endosome antigen 1 (EEA1) and lysosomal-associated membrane protein-1 (LAMP-1) were from Abcam Inc. (Cambridge, MA). All antibodies were used according to the manufacturer's specifications.
Primary human cerebral cortex astrocyte cells (used between passages 3-5, cat# 1800, ScienCell Research Laboratories, Carlsbad, CA) were cultured in cell specific astrocyte medium containing 2% fetal bovine serum, 1% astrocyte growth supplement and 1% penicillin/streptomycin (ScienCell Research Laboratories). Adherent human U-87 MG (used between passages 14-20, cat# HTB-14, American Type Culture Collection (ATCC), Rockville, MD), T98G (used between passages 10-20, cat# CRL-1690, ATCC), U-251 MG (used between passages 10-20, obtained from Dr. V.W. Yong, University of Calgary, Calgary, AB, Canada), U-373 MG (used between passages 10-20, cat# HTB-17, ATCC), and U-138 MG (used between passages 10-20, cat# HTB-16, ATCC) astrocytoma/glioblastoma cells were cultured in DMEM F12 + 1% L-glutamine (2 mM) (Cambrex, Walkersville, MD) supplemented with 10% fetal calf serum (Gibco, Burlington, ON, Canada), 1% penicillin-streptomycin (Gibco), 1% sodium pyruvate (1 mM) (Gibco) and 1% non-essential amino acids (0.1 mM) (Gibco) at 37°C and 5% CO2. Recently the relationship between U-251 MG and U-373 MG cell lines has come into question and analysis has suggested that they likely have a similar origin . Since these two cell lines are still in common use, we have considered them independently in this study but results obtained from these lines are interpreted in light of their apparent relationship.
Indirect Immunofluorescence (IIF)
Cells were cultured on poly-L-lysine coated coverslips (BD Falcon) for approximately 24 hours at 37°C and then fixed in 100% ice cold methanol for 10 minutes. To minimize non-specific binding of the antibodies, cells were incubated in a blocking buffer containing 10% normal goat serum (Antibodies Incorporated, Davis, CA) and 2% bovine serum albumin (Sigma-Aldrich) for 30 minutes at room temperature (RT). For colocalization studies, cells were incubated with primary antibodies at appropriate working dilutions for 30 minutes at RT. After washing with phosphate buffered saline (PBS), cells were incubated for 30 minutes in a dark chamber with the corresponding secondary goat fluorochrome-conjugated antibodies. Alexa Fluor (AF) 488 (green) or 568 (red) secondary antibodies were from Invitrogen (Burlington, ON, Canada). Subsequently, the slides were washed in several changes of PBS, cell nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI), mounted in Vectashield (Vector Laboratories, Burlingame, CA) and examined for IIF using a 100× objective on a Leica DMRE microscope equipped with epifluorescence and an Optronics camera. Figures were compiled in Adobe PhotoShop (version 7.0). Appropriate IIF controls with no or only one primary antibody or both secondary antibodies alone or in combination revealed no observable non-specific background staining and no detectable bleed-through between microscope filter sets.
Electron Microscopy (EM)
Cells were seeded into 35 mm dishes grown to confluence as monolayers over two days, then washed in PBS and fixed in 3% glutaraldehyde in Millonig's phosphate buffer for 1 hour at RT. Post-fixation was in 2% OsO4 for 20 minutes. The cells were dehydrated in ethanol, and then infiltrated with Polybed 812 resin (Polysciences). Polymerization was performed at 37°C for 24 hours. Silver-gray sections were cut with an ultramicrotome (Leica) equipped with a diamond knife, stained with uranyl acetate and lead citrate and then examined in a H-700 Hitachi electron microscope. Centriole lengths were obtained by direct measurements of centrioles on electron micrographs and correlated to scale bars automatically generated by the electron microscope on the same electron micrograph.
To establish a baseline for the evaluation of primary cilia in astrocytoma/glioblastoma cell lines we first investigated the structure and composition of primary cilia in a human primary astrocyte cell line using monospecific antibodies to known primary cilia proteins. These studies were then followed by ultrastructural analysis.
Human astrocytes express primary cilia that reside within a membrane bound channel herein termed the "cilium-pit" that is also the site of endocytosis
Using the aforementioned results as a starting point, the structure and expression of primary cilia was studied in five astrocytoma/glioblastoma cell lines: U-87 MG, T98G, U-251 MG, U-373 MG and U-138 MG using both IIF and electron microscopy.
Human astrocytoma/glioblastoma cell lines display defective ciliogenesis and centriole structure
Abnormalities in centriole structure occur in concert with abnormal ciliogenesis
The expression of a primary cilium relies on two events; 1) the activation of ciliogenesis and 2) the orderly progression through the stages of ciliogenesis so that a structurally and functionally competent mature cilium is formed [39–41]. Defects impacting one or both of these events may signal specific disease states. In this study normal primary astrocytes were found to express primary cilia and for the first time it was demonstrated that defects affecting both the activation of ciliogenesis as well as progression through the stages of ciliogenesis occur in cells derived from malignant astrocytomas/glioblastomas. In both cases, this appears to lead to arrest of normal primary cilium expression in these cells. We also found that the progression of ciliogenesis in astrocytoma/glioblastoma cells was unable to be forced or corrected by serum starvation; an observation suggesting that cessation of cell cycling does not affect the ability of astrocytomas/glioblastomas to proceed to later stages of ciliogenesis. In general, the failure to form a functional primary cilium can have at least two major implications: 1) it can alter the cells ability to properly sense and respond to its environment, and 2) it can impact the regulation of cell cycle progression. Thus, the non-communicative and unrestrained growth of astrocytoma/glioblastoma cells may be at least partially defined by defects in the process of ciliogenesis.
Aberrations in ciliogenesis and the resultant absence of mature primary cilia in astrocytoma/glioblastoma cells are not unique to this cell lineage. The absence of primary cilia has been noted in other tumors. For example, ciliogenesis was found to be suppressed in both pancreatic cancer cells and pancreatic intraepithelial neoplastic lesions in human pancreatic ductal adenocarcinoma (PDAC) as well as in three separate mouse models of PDAC driven by an endogenous oncogenic Kras allele . Interestingly, inhibition of the Kras effector pathway restored ciliogenesis suggesting that ciliogenesis in these cells may be actively repressed by oncogenic Kras. Unfortunately, the ultrastructure of these cells was not reported making it difficult to determine if these cells failed to initiate ciliogenesis or that they did so but then failed to proceed through ciliogenesis as observed in the astrocytomas/glioblastomas of our study.
Following the completion of our study and the submission of this manuscript two important studies have been published which directly relate to our findings [31, 32]. In the first study, the authors present evidence showing that primary cilia play a role in basal cell carcinoma (BCC). Specifically, ciliary ablation strongly inhibited BCC-like tumors induced by an activated form of Smo while removal of cilia accelerated tumors induced by activated Gli2, a transcriptional effector of Hedgehog (Hh) signaling. Similarly and directly related to our study, the study by Han et al. (2009) showed that genetic ablation of cilia is able to block medulloblastoma formation when the tumor was driven by a constitutively active Smo protein while removal of a cilium was required for medulloblastoma growth by a constitutively active Gli2 . Further, it was found that the presence or absence of cilia was associated with specific variants of human medulloblastoma primary cilia and were found in medulloblastomas with active Hh or WNT signaling but not in most medulloblastomas in other distinct molecular subgroups. Taken together, both of these studies demonstrate that primary cilia function as unique signaling organelles that can either mediate or suppress tumorigenesis depending on the nature of the oncogenic initiating event. Interestingly, Katayma et al. (2002) reported that Ptc and Smo mRNA expression in human astrocytic tumors are inversely correlated with malignancy histology and this relationship also applied to tumor-derived cell lines . This study also indicated that mRNA levels for Ptc and Smo proteins were weakly expressed in three of the five cell lines included in our study (U-87 MG, U-251 MG and T98G). Taken in context with our results, it would appear that the complex relationship of primary cilia, the Hh signaling pathway and tumorigenesis is likely a feature of astrocytoma cells. Importantly, our data expands previous studies by indicating that ciliogenesis is not an all or none processes but rather can be truncated at different stages based on underlying molecular defects. In the future, it will be important to determine to what degree specific defects in ciliogenesis progression impact tumorigenesis.
In addition to identifying ciliogenesis defects within astrocytoma/glioblastoma cell lines, our study also demonstrated the presence of structural aberrations in the centrioles of some astrocytoma/glioblastoma cell lines. Interestingly, Lingle and Salisbury (1999) reported unusually long centrioles similar to those reported here, as well as disruptions in the 'cartwheel' structure of the centriole in human breast cancer tissue . A recent report by Keller et al. (2009) has implicated the protein POC1 in the control of centiolar length . POC1 protein abnormalities may be a candidate in future studies of astrocytomas, glioblastomas or other cancer cells to determine if this protein is non-functional in cancer cells. Another recent study has shown that two centriolar proteins CPAP and CP110 play antagonizing roles in controlling the length of centrioles where overexpression of CPAP leads to strikingly long centrioles . Although it is unclear if ciliogenesis defects and centriole structural defects are related, it is interesting to note that unusual centriole length was correlated with aberrant distal and sub-distal appendages characteristic of T98G and U-87 MG cell lines (Figures 6 and 7). Our results suggest that alterations in centriole length may prevent the formation of properly structured appendages that are critical for basal body attachment to the plasma membrane. It has been reported that a lack of distal and sub-distal appendages in Odf2 (-/-) cells prevents docking of the mother centriole to the plasma membrane thus inhibiting ciliogenesis . Failure to attach properly to the plasma membrane may be related to defects of ciliogenesis and it is possible that the same defect(s) that forms the basis for aberrant ciliogenesis also impacts centriole structure.
A review of the literature reveals that primary cilia are either positioned at the surface of the cell so that the entire axoneme extends outward completely free of the cell body [48–50] or in a channel continuous with the plasma membrane so that only one third of the cilium extends outward from the cell surface [50–53]. Each configuration appears to be cell type specific and our study clearly shows that the internal configuration is characteristic of primary astrocytes. We also found this configuration in U-87 MG cells that only on rare instances displayed the latter stages of ciliogenesis.
To our knowledge, this is the first report to show that the astrocyte cilium-associated channel, which we have termed the cilium-pit, is a site of endocytosis. The association of the primary cilium with the cilium-pit may serve several functions. First, the pit may function to concentrate proteins, ions, hormones or neurotransmitters so that they can better exert their effects on the membranes of either the cilium-pit or the primary cilium. Second, the pit may allow endocytotic associated signaling pathways to be linked and/or coordinated with signaling pathways associated with the primary cilia. Thus, the formation of both a mature cilium and a cilium-pit in certain cell types may be crucial to essential cell signaling.
Recently Kovacs et al. (2008) reported that beta-arrestins are required to mediate the activity-dependent interaction of Smo and the kinesin motor protein Kif3A in the primary cilium . They showed that beta-arrestins are required for endocytosis of Smo and signaling to Gli transcription factors to mediate the effects of Hh on developmental processes which, if deregulated, may lead to tumorigenesis . When beta-arrestins were depleted using siRNA, Smo was no longer localized to the primary cilia and Gli was not activated , which suggested that endocytosis may be a key intracellular transport mechanism for the subcellular localization of signaling proteins. In future it will be important identify exactly which endocytic linked pathways function in the cilium-pit region.
It should be noted that the detection of a cilium-pit in astrocytes is reminiscent of the flagellar pocket found in single celled trypanosomes [55, 56]. Like the cilium-pit, the flagellar pocket is an invagination of the plasma membrane that forms a structure that encases the proximal end of a cilium and is an active site of secretion and endocytosis. However, the flagellar pocket differs from the cilium-pit in several ways. First, the flagellar pocket is associated with a motile rather than a non-motile cilium and the pocket is much shorter in length relative to the cilium. Second, the flagellar pocket has a highly structured neck region that is not found in the cilium-pit. Since the flagellar pocket is a hallmark of trypanosomes and has an organization that distinguishes it from the cilium-pit, we suggest that, in order to avoid confusion, it is appropriate to avoid using the flagellar pocket term to denote the region around the non-motile primary cilium found in human astrocytes.
The major finding of this report is that primary cilia are expressed by human astrocytes but this expression is disrupted generally during early ciliogenesis in cultured astrocytoma/glioblastoma cells. This finding is important for several reasons. First, it indicates that defects in primary cilium ciliogenesis do occur in astrocytomas and glioblastomas thus providing an impetus for further studies of the relationship between primary cilium defects and this type of malignancy. Second, it highlights the early events of ciliogenesis as a critical time in the ciliogenesis process and emphasizes the importance in not only identifying proteins that are critical to this period but investigating which of these proteins might be correlated with not only cancer but other known ciliopathies. Finally, our results show that primary cilia in astrocytes are adjacent to endosomal markers. Thus, the disruption in ciliogenesis also disrupts the formation of a site of endocytosis-based signaling.
We thank Leona Barclay, University of Calgary for her technical assistance. This work was supported in part by the Canadian Institutes for Health Research Grant MOP-57674 (MJF) and the Natural Sciences and Engineering Research Council of Canada Grant 690481 (JBR). MJF holds the Arthritis Society Chair. JJM is supported by a Canadian Institutes for Health Research Doctoral Research Award in the Area of Clinical Research and by an Alberta Heritage Foundation for Medical Research Studentship Award.
- Satir P, Christensen ST: Overview of Structure and Function of Mammalian Cilia. Annual Review of Physiology. 2007, 69: 377-400. 10.1146/annurev.physiol.69.040705.141236.View ArticlePubMedGoogle Scholar
- Davenport JR, Watts AJ, Roper VC, Croyle MJ, van Groen T, Wyss JM, Nagy TR, Kesterson RA, Yoder BK: Disruption of Intraflagellar Transport in Adult Mice Leads to Obesity and Slow-Onset Cystic Kidney Disease. Current Biology. 2007, 17: 1586-1594. 10.1016/j.cub.2007.08.034.View ArticlePubMedPubMed CentralGoogle Scholar
- Pazour GJ, Rosenbaum JL: Intraflagellar transport and cilia-dependent diseases. Trends in Cell Biology. 2002, 12: 551-555. 10.1016/S0962-8924(02)02410-8.View ArticlePubMedGoogle Scholar
- Blacque OE, Cevik S, Kaplan OI: Intraflagellar transport: from molecular characterisation to mechanism. Frontiers in Bioscience. 2008, 13: 2633-2652. 10.2741/2871.View ArticlePubMedGoogle Scholar
- Gorivodsky M, Mukhopadhyay M, Wilsch-Braeuninger M, Philips M, Teufel A, Kim C, Malik N, Huttner W, Westphal H: Intraflagellar transport protein 172 is essential for primary cilia formation and plays a vital role in patterning the mammalian brain. Developmental Biology. 2009, 325: 24-32. 10.1016/j.ydbio.2008.09.019.View ArticlePubMedGoogle Scholar
- Jurczyk A, Gromley A, Redick S, Agustin JS, Witman G, Pazour GJ, Peters DJM, Doxsey S: Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly. J Cell Biol. 2004, 166: 637-643. 10.1083/jcb.200405023.View ArticlePubMedPubMed CentralGoogle Scholar
- Miyoshi K, Onishi K, Asanuma M, Miyazaki I, Diaz-Corrales F, Ogawa N: Embryonic expression of pericentrin suggests universal roles in ciliogenesis. Development Genes and Evolution. 2006, 216: 537-542. 10.1007/s00427-006-0065-8.View ArticlePubMedGoogle Scholar
- Ishikawa H, Kubo A, Tsukita S, Tsukita S: Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary cilia. Nature Cell Biology. 2005, 7: 517-524. 10.1038/ncb1251.View ArticlePubMedGoogle Scholar
- Yoshimura Si, Egerer J, Fuchs E, Haas AK, Barr FA: Functional dissection of Rab GTPases involved in primary cilium formation. J Cell Biol. 2007, 178: 363-369. 10.1083/jcb.200703047.View ArticlePubMedPubMed CentralGoogle Scholar
- Huber D, Geisler S, Monecke S, Hoyer-Fender S: Molecular dissection of ODF2/Cenexin revealed a short stretch of amino acids necessary for targeting to the centrosome and the primary cilium. European Journal of Cell Biology. 2008, 87: 137-146. 10.1016/j.ejcb.2007.10.004.View ArticlePubMedGoogle Scholar
- Graser S, Stierhof YD, Lavoie SB, Gassner OS, Lamla S, Le Clech M, Nigg EA: Cep164, a novel centriole appendage protein required for primary cilium formation. J Cell Biol. 2007, 179: 321-330. 10.1083/jcb.200707181.View ArticlePubMedPubMed CentralGoogle Scholar
- Hearn T, Spalluto C, Phillips VJ, Renforth GL, Copin N, Hanley NA, Wilson DI: Subcellular Localization of ALMS1 Supports Involvement of Centrosome and Basal Body Dysfunction in the Pathogenesis of Obesity, Insulin Resistance, and Type 2 Diabetes. Diabetes. 2005, 54: 1581-1587. 10.2337/diabetes.54.5.1581.View ArticlePubMedGoogle Scholar
- Li G, Vega R, Nelms K, Gekakis N, Goodnow C, McNamara P, Wu H, Hong NA, Glynne R: A Role for Alstrom Syndrome Protein, Alms1, in Kidney Ciliogenesis and Cellular Quiescence. PLoS Genet. 2007, 3: e8-10.1371/journal.pgen.0030008.View ArticlePubMedPubMed CentralGoogle Scholar
- Schrøder JM, Schneider L, Christensen T, Pedersen LB: EB1 Is Required for Primary Cilia Assembly in Fibroblasts. Current Biology. 2007, 17: 1134-1139. 10.1016/j.cub.2007.05.055.View ArticlePubMedGoogle Scholar
- Tsang WY, Bossard C, Khanna H, Peranen J, Swaroop A, Malhotra V, Dynlacht BD: CP110 Suppresses Primary Cilia Formation through Its Interaction with CEP290, a Protein Deficient in Human Ciliary Disease. Developmental Cell. 2008, 15: 187-197. 10.1016/j.devcel.2008.07.004.View ArticlePubMedPubMed CentralGoogle Scholar
- Kim J, Krishnaswami SR, Gleeson JG: CEP290 interacts with the centriolar satellite component PCM-1 and is required for Rab8 localization to the primary cilium. Hum Mol Genet. 2008, 17: 3796-3805. 10.1093/hmg/ddn277.View ArticlePubMedPubMed CentralGoogle Scholar
- Gorden NT, Arts HH, Parisi MA, Coene KLM, Letteboer SJF, van Beersum SEC, Mans DA, Hikida A, Eckert M, Knutzen D, et al: CC2D2A Is Mutated in Joubert Syndrome and Interacts with the Ciliopathy-Associated Basal Body Protein CEP290. The American Journal of Human Genetics. 2008, 83: 559-571. 10.1016/j.ajhg.2008.10.002.View ArticlePubMedGoogle Scholar
- Michaud EJ, Yoder BK: The Primary Cilium in Cell Signaling and Cancer. Cancer Res. 2006, 66: 6463-6467. 10.1158/0008-5472.CAN-06-0462.View ArticlePubMedGoogle Scholar
- Pan J, Snell W: The Primary Cilium: Keeper of the Key to Cell Division. Cell. 2007, 129: 1255-1257. 10.1016/j.cell.2007.06.018.View ArticlePubMedGoogle Scholar
- Plotnikova OV, Golemis EA, Pugacheva EN: Cell Cycle-Dependent Ciliogenesis and Cancer. Cancer Res. 2008, 68: 2058-2061. 10.1158/0008-5472.CAN-07-5838.View ArticlePubMedPubMed CentralGoogle Scholar
- Satir P: Cilia Biology: Stop Overeating Now!. Current Biology. 2007, 17: R963-R965. 10.1016/j.cub.2007.09.006.View ArticlePubMedGoogle Scholar
- Ingham PW: Transducing Hedgehog: the story so far. EMBO J. 1998, 17: 3505-3511. 10.1093/emboj/17.13.3505.View ArticlePubMedPubMed CentralGoogle Scholar
- Dahmane N, Altaba A: Sonic hedgehog regulates the growth and patterning of the cerebellum. Development. 1999, 126: 3089-3100.PubMedGoogle Scholar
- Wallace VA: Purkinje-cell-derived Sonic hedgehog regulates granule neuron precursor cell proliferation in the developing mouse cerebellum. Current Biology. 1999, 9: 445-448. 10.1016/S0960-9822(99)80195-X.View ArticlePubMedGoogle Scholar
- Ou YY, Mack GJ, Zhang M, Rattner JB: CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation. J Cell Sci. 2002, 115: 1825-1835.PubMedGoogle Scholar
- Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DYR, Reiter JF: Vertebrate Smoothened functions at the primary cilium. Nature. 2005, 437: 1018-1021. 10.1038/nature04117.View ArticlePubMedGoogle Scholar
- Haycraft C, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK: Gli2 and Gli3 Localize to Cilia and Require the Intraflagellar Transport Protein Polaris for Processing and Function. PLoS Genet. 2005, 1: e53-10.1371/journal.pgen.0010053.View ArticlePubMedPubMed CentralGoogle Scholar
- Rohatgi R, Milenkovic L, Scott MP: Patched1 Regulates Hedgehog Signaling at the Primary Cilium. Science. 2007, 317: 372-376. 10.1126/science.1139740.View ArticlePubMedGoogle Scholar
- Danilov AI, Gomes-Leal W, Ahlenius H, Kokaia Z, Carlemalm E, Lindvall O: Ultrastructural and antigenic properties of neural stem cells and their progeny in adult rat subventricular zone. Glia. 2009, 57: 136-152. 10.1002/glia.20741.View ArticlePubMedGoogle Scholar
- Badano JL, Mitsuma N, Beales PL, Katsanis N: The Ciliopathies: An Emerging Class of Human Genetic Disorders. Annual Review of Genomics and Human Genetics. 2006, 7: 125-148. 10.1146/annurev.genom.7.080505.115610.View ArticlePubMedGoogle Scholar
- Wong SY, Seol AD, So PL, Ermilov AN, Bichakjian CK, Epstein EH, Dlugosz AA, Reiter JF: Primary cilia can both mediate and suppress Hedgehog pathway-dependent tumorigenesis. Nat Med. 2009, 15: 1055-1061. 10.1038/nm.2011.View ArticlePubMedPubMed CentralGoogle Scholar
- Han YG, Kim HJ, Dlugosz AA, Ellison DW, Gilbertson RJ, Alvarez-Buylla A: Dual and opposing roles of primary cilia in medulloblastoma development. Nat Med. 2009, 15: 1062-1065. 10.1038/nm.2020.View ArticlePubMedPubMed CentralGoogle Scholar
- Lacroix M: Persistant use of "false" cell lines. International Journal of Cancer. 2007, 122: 1-4. 10.1002/ijc.23233.View ArticleGoogle Scholar
- Piperno G, LeDizet M, Chang XJ: Microtubules containing acetylated alpha-tubulin in mammalian cells in culture. J Cell Biol. 1987, 104: 289-302. 10.1083/jcb.104.2.289.View ArticlePubMedGoogle Scholar
- Wehland J, Weber K: Turnover of the carboxy-terminal tyrosine of alpha-tubulin and means of reaching elevated levels of detyrosination in living cells. J Cell Sci. 1987, 88: 185-203.PubMedGoogle Scholar
- Wheatley DN, Feilen EM, Yin Z, Wheatley SP: Primary cilia in cultured mammalian cells: detection with an antibody against detyrosinated alpha-tubulin (ID5) and by electron microscopy. Journal of submicroscopic. 1994, 26: 91-102.Google Scholar
- Wheatley DN, Wang AM, Strugnell GE: Expression of primary cilia in mammalian cells. Cell Biology International. 1996, 20: 73-81. 10.1006/cbir.1996.0011.View ArticlePubMedGoogle Scholar
- Bishop GA, Berbari NF, Lewis J, Mykytyn K: Type III adenylyl cyclase localizes to primary cilia throughout the adult mouse brain. The Journal of Comparative Neurology. 2007, 505: 562-571. 10.1002/cne.21510.View ArticlePubMedGoogle Scholar
- Sorokin S: Centrioles and the formation of rudimentary cilia by fibroblasts and smooth muscle cells. J Cell Biol. 1962, 15: 363-377. 10.1083/jcb.15.2.363.View ArticlePubMedPubMed CentralGoogle Scholar
- Hagiwara H, Ohwada N, Aoki T, Takata K: Ciliogenesis and ciliary abnormalities. Medical Electron Microscopy. 2000, 33: 109-114. 10.1007/s007950000009.View ArticlePubMedGoogle Scholar
- Hagiwara H, Ohwada N, Takata K: Cell Biology of Normal and Abnormal Ciliogenesis in the Ciliated Epithelium. International Review of Cytology. Edited by: Kwang WJ. 2004, Academic Press, 234: 101-141. full_text.Google Scholar
- Ibrahim R, Messaoudi C, Chichon FJ, Celati C, Marco S: Electron tomography study of isolated human centrioles. Microscopy research and technique. 2009, 72: 42-48. 10.1002/jemt.20637.View ArticlePubMedGoogle Scholar
- Seeley ES, Carriere C, Goetze T, Longnecker DS, Korc M: Pancreatic Cancer and Precursor Pancreatic Intraepithelial Neoplasia Lesions Are Devoid of Primary Cilia. Cancer Res. 2009, 69: 422-430. 10.1158/0008-5472.CAN-08-1290.View ArticlePubMedPubMed CentralGoogle Scholar
- Katayama M, Yoshida K, Ishimori H, Katayama M, Kawase T, Motoyama J, Kamiguchi H: Patched and Smoothened MRNA Expression in Human Astrocytic Tumors Inversely Correlates with Histological Malignancy. Journal of Neuro-Oncology. 2002, 59: 107-115. 10.1023/A:1019660421216.View ArticlePubMedGoogle Scholar
- Lingle WL, Salisbury JL: Altered Centrosome Structure Is Associated with Abnormal Mitoses in Human Breast Tumors. Am J Pathol. 1999, 155: 1941-1951.View ArticlePubMedPubMed CentralGoogle Scholar
- Keller LC, Geimer S, Romijn E, Yates J, Zamora I, Marshall WF: Molecular Architecture of the Centriole Proteome: The Conserved WD40 Domain Protein POC1 Is Required for Centriole Duplication and Length Control. Mol Biol Cell. 2009, 20: 1150-1166. 10.1091/mbc.E08-06-0619.View ArticlePubMedPubMed CentralGoogle Scholar
- Schmidt TI, Kleylein-Sohn J, Westendorf J, Le Clech M, Lavoie SB, Stierhof YD, Nigg EA: Control of Centriole Length by CPAP and CP110. Current Biology. 2009, 19: 1005-1011. 10.1016/j.cub.2009.05.016.View ArticlePubMedGoogle Scholar
- Huang BQ, Masyuk TV, Muff MA, Tietz PS, Masyuk AI, LaRusso NF: Isolation and characterization of cholangiocyte primary cilia. Am J Physiol Gastrointest Liver Physiol. 2006, 291: G500-G509. 10.1152/ajpgi.00064.2006.View ArticlePubMedGoogle Scholar
- Poole CA, Flint MH, Beaumont BW: Analysis of the morphology and function of primary cilia in connective tissues: a cellular cybernetic probe?. Cell Motility. 1985, 5: 175-193. 10.1002/cm.970050302.View ArticlePubMedGoogle Scholar
- Collin SP, Barry Collin H: Primary cilia in vertebrate corneal endothelial cells. Cell Biology International. 2004, 28: 125-130. 10.1016/j.cellbi.2003.11.011.View ArticlePubMedGoogle Scholar
- Meek WD, Raber BT, McClain OM, McCosh JK, Baker BB: Fine structure of the human synovial lining cell in osteoarthritis: its prominent cytoskeleton. The Anatomical Record. 1991, 231: 145-155. 10.1002/ar.1092310202.View ArticlePubMedGoogle Scholar
- Shikichi M, Kitamura HP, Yanase H, Konno A, Takahashi-Iwanaga H, Iwanaga T: Three-dimensional Ultrastructure of Synoviocytes in the Horse Joint as Revealed by the Scanning Electron Microscope. Archives of Histology and Cytology. 1999, 62: 219-229. 10.1679/aohc.62.219.View ArticlePubMedGoogle Scholar
- Vandenabeele F, Bari CD, Moreels M, Lambrichts I, Accio F, Lippens PL, Luyten FP: Morphological and immunocytochemical characterization of cultured fibroblast-like cells derived from adult human synovial membrane. Archives of Histology and Cytology. 2003, 66: 145-153. 10.1679/aohc.66.145.View ArticlePubMedGoogle Scholar
- Kovacs JJ, Whalen EJ, Liu R, Xiao K, Kim J, Chen M, Wang J, Chen W, Lefkowitz RJ: beta-Arrestin-Mediated Localization of Smoothened to the Primary Cilium. Science. 2008, 320: 1777-1781. 10.1126/science.1157983.View ArticlePubMedPubMed CentralGoogle Scholar
- Lacomble S, Vaughan S, Gadelha C, Morphew MK, Shaw MK, McIntosh JR, Gull K: Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography. J Cell Sci. 2009, 122: 1081-1090. 10.1242/jcs.045740.View ArticlePubMedPubMed CentralGoogle Scholar
- Landfear SM, Ignatushchenko M: The flagellum and flagellar pocket of trypanosomatids. Molecular and Biochemical Parasitology. 2001, 115: 1-17. 10.1016/S0166-6851(01)00262-6.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/9/448/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.