- Research article
- Open Access
- Open Peer Review
Gene expression profiling of alveolar soft-part sarcoma (ASPS)
© Stockwin et al; licensee BioMed Central Ltd. 2009
- Received: 15 September 2008
- Accepted: 15 January 2009
- Published: 15 January 2009
Alveolar soft-part sarcoma (ASPS) is an extremely rare, highly vascular soft tissue sarcoma affecting predominantly adolescents and young adults. In an attempt to gain insight into the pathobiology of this enigmatic tumor, we performed the first genome-wide gene expression profiling study.
For seven patients with confirmed primary or metastatic ASPS, RNA samples were isolated immediately following surgery, reverse transcribed to cDNA and each sample hybridized to duplicate high-density human U133 plus 2.0 microarrays. Array data was then analyzed relative to arrays hybridized to universal RNA to generate an unbiased transcriptome. Subsequent gene ontology analysis was used to identify transcripts with therapeutic or diagnostic potential. A subset of the most interesting genes was then validated using quantitative RT-PCR and immunohistochemistry.
Analysis of patient array data versus universal RNA identified elevated expression of transcripts related to angiogenesis (ANGPTL2, HIF-1 alpha, MDK, c-MET, VEGF, TIMP-2), cell proliferation (PRL, IGFBP1, NTSR2, PCSK1), metastasis (ADAM9, ECM1, POSTN) and steroid biosynthesis (CYP17A1 and STS). A number of muscle-restricted transcripts (ITGB1BP3/MIBP, MYF5, MYF6 and TRIM63) were also identified, strengthening the case for a muscle cell progenitor as the origin of disease. Transcript differentials were validated using real-time PCR and subsequent immunohistochemical analysis confirmed protein expression for several of the most interesting changes (MDK, c-MET, VEGF, POSTN, CYP17A1, ITGB1BP3/MIBP and TRIM63).
Results from this first comprehensive study of ASPS gene expression identifies several targets involved in angiogenesis, metastasis and myogenic differentiation. These efforts represent the first step towards defining the cellular origin, pathogenesis and effective treatment strategies for this atypical malignancy.
- Vascular Endothelial Growth Factor
- Fibroblast Activation Protein
- Hepatocyte Growth Factor Receptor
Alveolar soft-part sarcoma (ASPS) is an extremely rare (1 diagnosis per 10 million population per year) soft tissue sarcoma with an indolent course and generally poor prognosis [1–3]. The disease manifests in young adults (15–35 years), where it is usually diagnosed from a painless mass in the lower limbs, head or neck [1, 4]. ASPS is surprisingly slow growing with a clinical course averaging 15 years, where late stage disease is associated with metastasis to multiple sites including the lungs, bone, lymph nodes and brain . The protracted ontogeny of ASPS makes it relatively resistant to classical chemotherapy and current treatment options are limited to surgical resection and localized radiotherapy.
Histologically, ASPS is composed of organoid nests of polygonal tumor cells encompassed by a dense capillary vasculature, giving rise to the 'alveolar' appearance [1, 2]. In spite of several reports suggesting ASPS arises from a myogenic progenitor, the tissue ontogeny of ASPS is still contested, primarily because consistent detection of muscle markers (e.g. MYOD1 or MYOG) has not been demonstrated [5, 6]. ASPS cells also contain numerous periodic acid Schiff-positive, diastase-resistant crystalline structures, recently identified as containing monocarboxylate transporter 1 (MCT1) and the cellular chaperone CD147 .
At the cytogenetic level, ASPS exhibits a conserved abnormality in the form of an unbalanced translocation der(17)t(X;17)(p11;q25) which fuses the N-terminal region of the alveolar soft part locus gene (ASPL), located at 17q25, to the C-terminal region of the transcription factor E3 (TFE3), located at Xp11. Two alternative fusion junctions have been observed resulting in the expression of two distinct fusion transcripts, ASPL-TFE3 type 1 and type 2 . TFE3 is a ubiquitous basic helix-loop-helix (bHLH) leucine zipper transcription factor, which binds positive strand e-box motifs [CANNTG] . High-affinity interactions with DNA occur when TFE3 is present as a homodimer or when combined with other bHLH factors including MITF, TFEB and TFEC . Conversely, ASPL has only been partially characterized as a tethering protein that forces retention of GLUT4-containing vesicles in the cytoplasm in the absence of insulin [10, 11].
The current consensus is that aberrant TFE3 transcriptional activity modulated by the ASPL fusion partner is likely responsible for elements of ASPS pathogenesis. Further support for the primacy of TFE3 comes from the detection of alternate TFE3 fusion proteins (PRCC-TFE3, PSF-TFE3, NONO-TFE3) in a subset of renal carcinomas [12–15]. As regards novel properties of ASPL-TFE3, one study using an anti-TFE3 antibody demonstrated 'aberrantly strong' nuclear staining in ASPS patients suggesting either enhanced nuclear trafficking, protein stability or expression levels . More recently, fusion proteins containing TFE3 have been shown to directly activate transcription of c-MET (Hepatocyte growth factor receptor) through a consensus e-box . Given that c-MET plays an important role in cell survival and angiogenesis, this important observation forges a link between ASPL-TFE3 and disease pathogenesis, intimating that genes with similar promoter elements may also be activated .
Therefore, as a slow growing yet highly vascular sarcoma, ASPS is an intriguing candidate for gene expression analysis. In this study, freshly isolated patient RNA was used to generate a transcriptomic profile for ASPS. Results demonstrated elevated expression for factors involved in angiogenesis including: angiopoietin-like 2 (ANGPTL2), the HGF receptor (c-MET), Hypoxia-Inducible factor 1α (HIF1A), midkine (MDK) and vascular endothelial growth factors A/B (VEGF/VEGFB). Further exploration identified muscle-specific beta 1 integrin binding protein (ITGB1BP3/MIBP), muscle-specific RING finger protein 1 (TRIM63) and Myogenic factors 5 and 6 (MYF5/6) as potential ASPS markers. Overall, these data identify molecular targets with the potential to aid the diagnosis and treatment of ASPS.
A list of antibody sources, dilutions, retrieval conditions and positive control tissues used in immunohistochemical analysis is provided [see Additional file 1]. Unless otherwise indicated in the following methods, all other chemicals and inhibitors were from Sigma (St. Louis, MO).
ASPS diagnosis and RNA isolation
Tumors were obtained from surgery following informed consent under NCI clinical research protocol 05-C-N138, approved by the U.S. National Cancer Institute (NCI) Institutional Review Board (IRB), and with the assistance of the Alliance Against Alveolar Soft-Part Sarcoma (TAAASPS). This research is in compliance with the Helsinki Declaration for conduct of research utilizing human subjects. A representative tumor sample (0.5–1.0 cm) was fixed in buffered 10% formalin for histopathology. The following criteria were employed for the diagnosis of ASPS: the presence of organoid nests of cells surrounded by endothelial-lined vascular channels in H&E stained material ; cytoplasmic PAS positive crystals following digestion with diastase ; the presence of the ASPL-TFE3 type 1 or type 2 fusion transcript , nuclear staining with antibodies to the transcription factor TFE3  and to either the ASPL-TFE3 type 1 or type 2 fusion protein . For isolation of RNA, a portion of tumor tissue was immediately placed in RNA later solution (Ambion, Austin, TX) and stored at 4°C. Total RNA was prepared using the RNeasy Fibrous Midi Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. The concentration of RNA was determined on a Nanodrop spectrophotometer, and the A260/A280 values were greater than 1.9. The integrity and size distribution of RNA were evaluated on 1% denaturing NuSieve agarose gels (FMC, Rockland, ME) followed by ethidium bromide staining.
Total RNA was isolated from cells using the RNeasy mini kit (Qiagen, Valencia, CA) and reverse transcribed using Omniscript RT according to the manufacturers' instructions. A standard reaction comprised 2 μg total RNA, 0.5 mM each dNTP, 2 μM random decamers (Ambion, Austin, TX) and 4 units of reverse transcriptase in 20 μl total volume of 1× RT buffer. The reaction was allowed to proceed for 90 min at 37°C and the cDNA product diluted to 1 μg/ml and stored at -80°C.
Microarray data acquisition
Microarray data was collected at Expression Analysis, Inc. (Durham, NC) using the GeneChip® Human U133 plus 2.0 Array (Affymetrix), according to standardized operating procedures. Overall, analysis was performed on RNA from seven ASPS patients and universal RNA, where all samples were analyzed in duplicate. The universal reference RNA (Clontech) is a mixture of RNAs from a collection of adult human tissues (male and female) representing a broad range of expressed genes. Affymetrix array data files (.CEL) have been deposited with the Gene Expression omnibus http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE13433.
Microarray data analysis
The Genesifter suite (VizX labs, Seattle, WA) was used for analysis of microarray data. In brief, compressed .CEL files containing array data were loaded into the Genesifter web portal http://www.genesifter.net. Using the advanced upload function, probe-level data was then compiled, normalized and transformed using GC-RMA . Pairwise analysis was then performed comparing universal RNA arrays with ASPS patient arrays. The following criteria were applied to filter the differentially expressed transcript list, a fold change of >3 and a Wilcoxon rank sum test where p < 0.05 with Benjamini-Hochberg estimation of false discovery rate (FDR). The list of differentially regulated transcripts was then exported into excel for gene ontogeny analysis.
SYBR Green chemistry was used to detect primer specific amplicons. For each reaction, 12.5 μl Quantitect SYBR Green PCR mastermix (Qiagen, Valencia, CA) was diluted 1:2 in DNase free water containing 5 ng cDNA and 1 μM of specific primer pair. Reactions were performed in triplicate and universal 18S RNA primers (Ambion, Austin, TX) were used to normalize cDNA amplification. The fluorochrome ROX, included in the PCR mastermix, was used as a passive reference. Reactions were performed using an ABI7500 thermocycler (Applied Biosystems, Foster City, CA). Cycling conditions consisted of a single 10 min/95°C activation step followed by 50 cycles of 95°C/15 s, 60°C/60 s and 72°C/60 s with fluorescence measurements taken in the elongation step of each cycle. Fold changes and p values were then calculated by copying CT data into a Superarray analysis template (Superarray Inc. Frederick, MD). For each primer pair melting curve analysis was used to confirm the presence of a single amplicon. Sequences of primers used in quantitative RT-PCR are provided [see Additional file 2].
In brief, tissue sections (5 μm) affixed to glass slides, were deparaffinized and treated for 15 minutes in methanol containing 0.6% H2O2 followed by either 1 mM EDTA (pH 8.0) or 10 mM citrate buffer (pH 6.0) in a microwave vacuum histoprocessor (RHS-1; Milestone, Italy). The temperature was gradually increased to 100°C over 10 minutes and maintained at 100°C for an additional 10 minutes. Sections were cooled, rinsed sequentially in H2O and PBS for 10 minutes each. Primary antibodies, used at dilutions indicated [see Additional file 1] were overlayed onto the tissue sections and incubated either overnight or for 30 min at room temperature. Sections were rinsed in PBS for 10 minutes and incubated with the appropriate secondary antibody for 30 minutes. Sections were rinsed in PBS for 10 minutes and processed according to instructions detailed in the detection kits.
ASPS Patient Demographics
ASPL-TFE3 Fusion Type
Primary or Metastasis
Validation and gene ontology analysis
Altered expression of transcripts involved in the response to hypoxia and angiogenesis suggests that these pathways are active in ASPS and provides some of the most interesting candidates for therapeutic intervention. The greatest increase in angiogenesis-related genes was seen for angiopoietin-like 2 (ANGPTL2). Angiopoietins are hypoxia-regulated members of the vascular endothelial growth factor (VEGF) family, where ANGPTL2 acts as an endothelial survival factor that does not bind TIE1 or TIE2 . Increased expression was also observed for the master transcriptional regulator of the response to hypoxia, HIF-1α (HIF1A), which is interesting given that regulation of HIF-1α occurs predominantly through a post-translational mechanism . Similarly, enhanced expression of Midkine (MDK), a hypoxia-induced growth factor involved in cell survival and migration, provides a potential target for inhibition . Here, we also confirm consistent increases in expression of the hepatocyte growth factor receptor (MET). In a recent study, ASPL-TFE3 and other TFE3 fusion proteins were shown to bind and strongly activate the MET promoter . Furthermore, in cells expressing TFE3, inhibition of MET using siRNA or a small molecule MET inhibitor impaired tumor growth. A similarly important observation concerned elevated expression of two members of the VEGF family, VEGF-A and VEGF-B, supporting the utility of inhibitors such as Bevacizumab in the treatment of ASPS . Increased expression of Stabilin (STAB1) may also contribute towards an angiogenic phenotype, given that this scavenger receptor is expressed on endothelial cells and inhibition with monoclonal antibodies has been shown to prevent cord formation in vitro . In a recent study, RNA isolated from formalin-fixed paraffin-embedded ASPS tumor material was subjected to focused microarray analysis with emphasis on genes involved in angiogenesis . Overall, 18 genes were found to be upregulated >1.5 fold with 8 regulated >3 fold. Cross-comparison showed that 7/18 of these genes (MDK, PTN, JAG1, TGFB1, NRP1, HIF1A and TIMP2) were represented in our transcriptome (which utilizes a 3-fold cut-off). These results are noteworthy as they confirm several of our own observations and highlight a possible role for JAG1. Overall, identification of several angiogenic mediators (Angiopoietin-like 2, c-MET, HIF-1α and VEGF) provides a molecular framework for ASPS vascular histology and supports the use of anti-angiogenic agents in treatment .
Increased expression of several mitogenic factors was also noted. One example was Prolactin (PRL), a 23 kDa peptide hormone implicated in a diverse range of processes including lactation and cell proliferation . Prolactin is recognized as a breast cancer cell survival factor and prolactin inhibitors demonstrate the ability to inhibit cell proliferation in vitro and to slow tumor growth in mice [28, 29]. Further transcripts involved in growth regulation showing upregulation included; insulin like growth factor binding protein 1 (IGFBP1), Neurotensin receptor 2 (NTSR2) and proprotein convertase subtilisin/kexin type 1 (PCSK1).
Several genes broadly implicated in metastasis showed elevated expression. Periostin (POSTN) is a 90 kDa disulphide-linked secreted protein which has homology to the axon guidance protein fasciclin I . Periostin is expressed predominantly in fetal tissues and also in ovarian cancer, where it binds αVβ3/αVβ5 integrins and promotes cellular motility . Neutralizing therapeutic antibodies directed against Periostin have already undergone pre-clinical evaluation, with promising results . Extracellular matrix protein 1 (ECM1) meanwhile is a recently identified secreted glycoprotein, over-expressed in several epithelial malignancies, recognized as capable of promoting metastasis and angiogenesis . A disintegrin and metalloproteinase domain 9 (ADAM9) is a membrane bound metalloprotease, which like the other ADAM family members plays a role in cellular invasion, metastasis and angiogenesis . Along with proteolytic activity, ADAM9 appears to function as an adhesion molecule mediating interactions with αvβ5 integrin . This protein is expressed in a variety of normal tissues and shows elevated expression in certain cancers and under conditions of cellular stress .
Aberrant expression was also noted for transcripts implicated in embryonic development. Pregnancy-specific glycoprotein 9 (PSG9) is a secreted glycoprotein belonging to the CEA family. In colorectal cancer, PSG9 also shows elevated expression . However, the function of the antigen is largely unknown, although an immunological role has been suggested . Equally, glycoprotein NMB (GPNMB) is a type I membrane protein with homology to the melanoma antigen pMEL17 . This antigen has been identified at the plasma membrane of several malignancies including melanoma and glioma [39, 40]. A humanized toxin-conjugated NMB antibody was shown to be effective in killing antigen-positive melanoma, suggesting that this therapeutic may have potential in the treatment of other NMB positive malignancies . Enhanced expression was observed for Inhibin beta E chain (INHBE), a subunit which contributes towards the activin/inhibin pleotropic hormone axis. Inhibins are heterodimers of one α and one β subunit, whereas activins are homo- or heterodimers of β subunits only. As members of the extended TGF-β super family, these activins play a number of roles including cell proliferation, differentiation and apoptosis . Two genes involved in steroid biosynthesis, cytochrome P450-17A1 (CYP17A1) and Steroid sulfatase (STS), also show increased expression. CYP17A1 is an enzyme that converts pregnenolone and progesterone to their 17- alpha-hydroxylated products whereas STS catalyzes the conversion of sulfated steroid precursors to estrogens.
With respect to defining ASPS cellular ontogeny, Symatlas identified a preponderance of muscle-restricted genes in the ASPS transcriptome (results not shown). Classical markers of skeletal muscle development (myogenin, myoblast determination protein 1, desmin and myoglobin) were not detected at the mRNA level, mirroring reports obtained elsewhere . However, myogenic factors 5 and 6 (MYF5/6), important members of the myogenic basic helix-loop-helix (bHLH) family of transcription factors, were upregulated. Interestingly, myogenic factors such as these bind to the same E-box consensus motif [CANNTG] as TFE3 . In terms of myogenic differentiation, evidence suggests that MYF5 is involved in progenitor cell proliferation whereas myoblast determination protein 1 and myogenin are concerned more with cell specialization and terminal differentiation . A further compelling candidate for an ASPS marker was Muscle-Specific B1 Integrin Binding Protein (ITGB1BP3/MIBP). Expression of this transcript was significantly increased in ASPS relative to universal RNA (1238 up microarray; 482 up QPCR) and according to Symatlas is expressed primarily in skeletal muscle and heart. A possible link to ASPS pathogenesis may be inferred from one recent study showing that in myoblasts, ITGB1BP3 suppresses fusion and terminal differentiation . This protein appears to function by selectively binding the α7β1 v laminin receptor and mediating reduced laminin cell adhesion and matrix deposition . Similarly, muscle specific enolase 3 (ENO3) was upregulated in all patients. This enzyme is a HIF-1α inducible gene thought to be involved in striated muscle development and regeneration [47, 48]. Increased expression was similarly observed for Muscle-specific RING finger protein 1 (TRIM63), an E3 ubiquitin ligase involved in the degradation of sarcomere-associated proteins such as troponin . In combination with atrogin-1, this protein is thought to be involved in skeletal muscle atrophy . A further upregulated skeletal muscle restricted transcript was Nebulin (NEB), a giant (800 kDa) protein that maintains the structural integrity of sarcomeres in skeletal muscle . ASPS cells are reported to contain cytoplasmic granules containing monocarboxylate transporter 1 (MCT1) and the accessory protein Basigin (BSG/CD147) . A search of our array data identified increased expression of MCT1 but not CD147.
A group of smooth muscle restricted transcripts were similarly upregulated. Fibroblast activation protein α (FAP) is a gelatinase implicated in tissue remodeling and tumor invasion expressed primarily in smooth muscle and also found in some melanoma cell lines . Sushi-repeat-containing protein, X-linked 2 (SRPX2) is a poorly characterized protein expressed primarily in smooth muscle and neuronal tissue . Finally, lysyl hydroxylase 2 (PLOD2) is responsible for the production of stabilizing hydroxylysine residues in collagens and shows highest expression in smooth muscle .
Therefore, detection of two myogenic regulatory factors MYF5/MYF6 along with several other muscle-specific transcripts including ITGB1BP3/MIBP and TRIM63 seems to favor a myogenic progenitor . Several reports have explored the possible myogenic origin of ASPS [55–61]. Within these, there are conflicting reports of expression of the muscle-specific factor MyoD1 [58–60]. Our data suggest that MyoD1 is absent whereas family members MYF5 and MYF6 are expressed. We also concur with results showing that ASPS is myogenin negative . Therapeutic targeting of muscle-specific protein may also have potential. For example, experiments using siRNAs are currently underway to determine whether the reported ability of ITGB1BP3/MIBP to inhibit myoblasts terminal differentiation also extends to ASPS cells .
In conclusion, the work presented here comprises the first genome-wide microarray analysis of ASPS; generated using RNA isolated from tumors at the time of surgery. The approach used strongly suggests that for rare tumors such as ASPS, microarray analysis of small number of fresh tumor RNA samples is preferable to analysis of RNA from large numbers of archival specimens. Results identify targets with diagnostic/therapeutic potential and suggest a myogenic progenitor as the origin of disease. Our ongoing efforts towards classification of ASPS in the context of other soft-tissue sarcomas and the development of an ASPS cell line will further address the issue of cellular origin. An equally important question concerns the exact role of the ASPL-TFE3 fusion protein in tumorigenesis. The ability of fusion proteins containing TFE3 to transactivate e-box containing genes such as c-MET suggests that certain facets of the transcriptome are related to ASPL-TFE3 activity . It is hoped that by making this array data publically available we will promote additional investigations centered on this unique malignancy and provide a useful reference dataset for the wider classification of soft-tissue sarcomas.
This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. NO1-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. This research was supported [in part] by the Developmental Therapeutics Program in the Division of Cancer Treatment and Diagnosis of the National Cancer Institute. We also recognize the intellectual contributions to this manuscript made by Dr. Ming Zhao (NCI, Bethesda).
- Christopherson WM, Foote FW, Stewart FW: Alveolar soft-part sarcomas; structurally characteristic tumors of uncertain histogenesis. Cancer. 1952, 5 (1): 100-111. 10.1002/1097-0142(195201)5:1<100::AID-CNCR2820050112>3.0.CO;2-K.View ArticlePubMedGoogle Scholar
- Folpe AL, Deyrup AT: Alveolar soft-part sarcoma: a review and update. J Clin Pathol. 2006, 59 (11): 1127-1132. 10.1136/jcp.2005.031120.View ArticlePubMedPubMed CentralGoogle Scholar
- Soft Tissue Sarcomas – SEER Pediatric Monograph. [http://www.seer.cancer.gov]
- Kayton ML, Meyers P, Wexler LH, Gerald WL, LaQuaglia MP: Clinical presentation, treatment, and outcome of alveolar soft part sarcoma in children, adolescents, and young adults. J Pediatr Surg. 2006, 41 (1): 187-193. 10.1016/j.jpedsurg.2005.10.023.View ArticlePubMedGoogle Scholar
- Wang NP, Bacchi CE, Jiang JJ, McNutt MA, Gown AM: Does alveolar soft-part sarcoma exhibit skeletal muscle differentiation? An immunocytochemical and biochemical study of myogenic regulatory protein expression. Mod Pathol. 1996, 9 (5): 496-506.PubMedGoogle Scholar
- Gomez JA, Amin MB, Ro JY, Linden MD, Lee MW, Zarbo RJ: Immunohistochemical profile of myogenin and MyoD1 does not support skeletal muscle lineage in alveolar soft part sarcoma. Arch Pathol Lab Med. 1999, 123 (6): 503-507.PubMedGoogle Scholar
- Ladanyi M, Antonescu CR, Drobnjak M, Baren A, Lui MY, Golde DW, Cordon-Cardo C: The precrystalline cytoplasmic granules of alveolar soft part sarcoma contain monocarboxylate transporter 1 and CD147. Am J Pathol. 2002, 160 (4): 1215-1221.View ArticlePubMedPubMed CentralGoogle Scholar
- Ladanyi M, Lui MY, Antonescu CR, Krause-Boehm A, Meindl A, Argani P, Healey JH, Ueda T, Yoshikawa H, Meloni-Ehrig A, Sorensen PH, Mertens F, Mandahl N, Berghe van den H, Sciot R, Cin PD, Bridge J: The der(17)t(X;17)(p11;q25) of human alveolar soft part sarcoma fuses the TFE3 transcription factor gene to ASPL, a novel gene at 17q25. Oncogene. 2001, 20 (1): 48-57. 10.1038/sj.onc.1204074.View ArticlePubMedGoogle Scholar
- Henthorn PS, Stewart CC, Kadesch T, Puck JM: The gene encoding human TFE3, a transcription factor that binds the immunoglobulin heavy-chain enhancer, maps to Xp11.22. Genomics. 1991, 11 (2): 374-378.View ArticlePubMedGoogle Scholar
- Kuiper RP, Schepens M, Thijssen J, Schoenmakers EF, van Kessel AG: Regulation of the MiTF/TFE bHLH-LZ transcription factors through restricted spatial expression and alternative splicing of functional domains. Nucleic Acids Res. 2004, 32 (8): 2315-2322. 10.1093/nar/gkh571.View ArticlePubMedPubMed CentralGoogle Scholar
- Tettamanzi MC, Yu C, Bogan JS, Hodsdon ME: Solution structure and backbone dynamics of an N-terminal ubiquitin-like domain in the GLUT4-regulating protein, TUG. Protein Sci. 2006, 15 (3): 498-508. 10.1110/ps.051901806.View ArticlePubMedPubMed CentralGoogle Scholar
- Sidhar SK, Clark J, Gill S, Hamoudi R, Crew AJ, Gwilliam R, Ross M, Linehan WM, Birdsall S, Shipley J, Cooper CS: The t(X;1)(p11.2;q21.2) translocation in papillary renal cell carcinoma fuses a novel gene PRCC to the TFE3 transcription factor gene. Hum Mol Genet. 1996, 5 (9): 1333-1338. 10.1093/hmg/5.9.1333.View ArticlePubMedGoogle Scholar
- Weterman MA, Wilbrink M, Geurts van Kessel A: Fusion of the transcription factor TFE3 gene to a novel gene, PRCC, in t(X;1)(p11;q21)-positive papillary renal cell carcinomas. Proc Natl Acad Sci USA. 1996, 93 (26): 15294-15298. 10.1073/pnas.93.26.15294.View ArticlePubMedPubMed CentralGoogle Scholar
- Argani P, Antonescu CR, Couturier J, Fournet JC, Sciot R, Debiec-Rychter M, Hutchinson B, Reuter VE, Boccon-Gibod L, Timmons C, Hafez N, Ladanyi M: PRCC-TFE3 renal carcinomas: morphologic, immunohistochemical, ultrastructural, and molecular analysis of an entity associated with the t(X;1)(p11.2;q21). Am J Surg Pathol. 2002, 26 (12): 1553-1566. 10.1097/00000478-200212000-00003.View ArticlePubMedGoogle Scholar
- Clark J, Lu YJ, Sidhar SK, Parker C, Gill S, Smedley D, Hamoudi R, Linehan WM, Shipley J, Cooper CS: Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma. Oncogene. 1997, 15 (18): 2233-2239. 10.1038/sj.onc.1201394.View ArticlePubMedGoogle Scholar
- Argani P, Lal P, Hutchinson B, Lui MY, Reuter VE, Ladanyi M: Aberrant nuclear immunoreactivity for TFE3 in neoplasms with TFE3 gene fusions: a sensitive and specific immunohistochemical assay. Am J Surg Pathol. 2003, 27 (6): 750-761. 10.1097/00000478-200306000-00005.View ArticlePubMedGoogle Scholar
- Tsuda M, Davis IJ, Argani P, Shukla N, McGill GG, Nagai M, Saito T, Lae M, Fisher DE, Ladanyi M: TFE3 fusions activate MET signaling by transcriptional up-regulation, defining another class of tumors as candidates for therapeutic MET inhibition. Cancer Res. 2007, 67 (3): 919-929. 10.1158/0008-5472.CAN-06-2855.View ArticlePubMedGoogle Scholar
- Desiderio MA: Hepatocyte growth factor in invasive growth of carcinomas. Cell Mol Life Sci. 2007, 64 (11): 1341-1354. 10.1007/s00018-007-7050-x.View ArticlePubMedGoogle Scholar
- Vistica DT, Krosky PM, Kenney S, Raffeld M, Shoemaker RH: Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma. J Pediatr Hematol Oncol. 2008, 30 (1): 46-52.View ArticlePubMedGoogle Scholar
- Wu Z, Irizarry RA: Stochastic models inspired by hybridization theory for short oligonucleotide arrays. J Comput Biol. 2005, 12 (6): 882-893. 10.1089/cmb.2005.12.882.View ArticlePubMedGoogle Scholar
- Kim I, Moon SO, Koh KN, Kim H, Uhm CS, Kwak HJ, Kim NG, Koh GY: Molecular cloning, expression, and characterization of angiopoietin-related protein. angiopoietin-related protein induces endothelial cell sprouting. J Biol Chem. 1999, 274 (37): 26523-26528. 10.1074/jbc.274.37.26523.View ArticlePubMedGoogle Scholar
- Harris AL: Hypoxia–a key regulatory factor in tumour growth. Nat Rev Cancer. 2002, 2 (1): 38-47. 10.1038/nrc704.View ArticlePubMedGoogle Scholar
- Kadomatsu K: The midkine family in cancer, inflammation and neural development. Nagoya J Med Sci. 2005, 67: 3-4.Google Scholar
- Azizi AA, Haberler C, Czech T, Gupper A, Prayer D, Breitschopf H, Acker T, Slavc I: Vascular-endothelial-growth-factor (VEGF) expression and possible response to angiogenesis inhibitor bevacizumab in metastatic alveolar soft part sarcoma. Lancet Oncol. 2006, 7 (6): 521-523. 10.1016/S1470-2045(06)70729-X.View ArticlePubMedGoogle Scholar
- Adachi H, Tsujimoto M: FEEL-1, a novel scavenger receptor with in vitro bacteria-binding and angiogenesis-modulating activities. J Biol Chem. 2002, 277 (37): 34264-34270. 10.1074/jbc.M204277200.View ArticlePubMedGoogle Scholar
- Lazar AJ, Das P, Tuvin D, Korchin B, Zhu Q, Jin Z, Warneke CL, Zhang PS, Hernandez V, Lopez-Terrada D, Pisters PW, Pollock RE, Lev D: Angiogenesis-promoting gene patterns in alveolar soft part sarcoma. Clin Cancer Res. 2007, 13 (24): 7314-7321. 10.1158/1078-0432.CCR-07-0174.View ArticlePubMedGoogle Scholar
- Van Coppenolle F, Skryma R, Ouadid-Ahidouch H, Slomianny C, Roudbaraki M, Delcourt P, Dewailly E, Humez S, Crepin A, Gourdou I, Djiane J, Bonnal JL, Mauroy B, Prevarskaya N: Prolactin stimulates cell proliferation through a long form of prolactin receptor and K+ channel activation. Biochem J. 2004, 377 (Pt 3): 569-578.View ArticlePubMedPubMed CentralGoogle Scholar
- Beck MT, Chen NY, Franek KJ, Chen WY: Prolactin antagonist-endostatin fusion protein as a targeted dual-functional therapeutic agent for breast cancer. Cancer Res. 2003, 63 (13): 3598-3604.PubMedGoogle Scholar
- Perks CM, Keith AJ, Goodhew KL, Savage PB, Winters ZE, Holly JM: Prolactin acts as a potent survival factor for human breast cancer cell lines. Br J Cancer. 2004, 91 (2): 305-311.PubMedPubMed CentralGoogle Scholar
- Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, Toyama Y, Bonewald LF, Kudo A: Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. J Bone Miner Res. 1999, 14 (7): 1239-1249. 10.1359/jbmr.1918.104.22.1689.View ArticlePubMedGoogle Scholar
- Gillan L, Matei D, Fishman DA, Gerbin CS, Karlan BY, Chang DD: Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility. Cancer Res. 2002, 62 (18): 5358-5364.PubMedGoogle Scholar
- Tai IT, Dai M, Chen LB: Periostin induction in tumor cell line explants and inhibition of in vitro cell growth by anti-periostin antibodies. Carcinogenesis. 2005, 26 (5): 908-915. 10.1093/carcin/bgi034.View ArticlePubMedGoogle Scholar
- Han Z, Ni J, Smits P, Underhill CB, Xie B, Chen Y, Liu N, Tylzanowski P, Parmelee D, Feng P, Ding I, Gao F, Gentz R, Huylebroeck D, Merregaert J, Zhang L: Extracellular matrix protein 1 (ECM1) has angiogenic properties and is expressed by breast tumor cells. Faseb J. 2001, 15 (6): 988-994. 10.1096/fj.99-0934com.View ArticlePubMedGoogle Scholar
- Mochizuki S, Okada Y: ADAMs in cancer cell proliferation and progression. Cancer Sci. 2007, 98 (5): 621-628. 10.1111/j.1349-7006.2007.00434.x.View ArticlePubMedGoogle Scholar
- Zhou M, Graham R, Russell G, Croucher PI: MDC-9 (ADAM-9/Meltrin gamma) functions as an adhesion molecule by binding the alpha(v)beta(5) integrin. Biochem Biophys Res Commun. 2001, 280 (2): 574-580. 10.1006/bbrc.2000.4155.View ArticlePubMedGoogle Scholar
- Sung SY, Kubo H, Shigemura K, Arnold RS, Logani S, Wang R, Konaka H, Nakagawa M, Mousses S, Amin M, Anderson C, Johnstone P, Petros JA, Marshall FF, Zhau HE, Chung LW: Oxidative stress induces ADAM9 protein expression in human prostate cancer cells. Cancer Res. 2006, 66 (19): 9519-9526. 10.1158/0008-5472.CAN-05-4375.View ArticlePubMedGoogle Scholar
- Salahshor S, Goncalves J, Chetty R, Gallinger S, Woodgett JR: Differential gene expression profile reveals deregulation of pregnancy specific beta1 glycoprotein 9 early during colorectal carcinogenesis. BMC Cancer. 2005, 5: 66-10.1186/1471-2407-5-66.View ArticlePubMedPubMed CentralGoogle Scholar
- Snyder SK, Wessner DH, Wessells JL, Waterhouse RM, Wahl LM, Zimmermann W, Dveksler GS: Pregnancy-specific glycoproteins function as immunomodulators by inducing secretion of IL-10, IL-6 and TGF-beta1 by human monocytes. Am J Reprod Immunol. 2001, 45 (4): 205-216. 10.1111/j.8755-8920.2001.450403.x.View ArticlePubMedGoogle Scholar
- Weterman MA, Ajubi N, van Dinter IM, Degen WG, van Muijen GN, Ruitter DJ, Bloemers HP: nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts. Int J Cancer. 1995, 60 (1): 73-81. 10.1002/ijc.2910600111.View ArticlePubMedGoogle Scholar
- Kuan CT, Wakiya K, Dowell JM, Herndon JE, Reardon DA, Graner MW, Riggins GJ, Wikstrand CJ, Bigner DD: Glycoprotein nonmetastatic melanoma protein B, a potential molecular therapeutic target in patients with glioblastoma multiforme. Clin Cancer Res. 2006, 12: 1970-1982. 10.1158/1078-0432.CCR-05-2797.View ArticlePubMedGoogle Scholar
- Tse KF, Jeffers M, Pollack VA, McCabe DA, Shadish ML, Khramtsov NV, Hackett CS, Shenoy SG, Kuang B, Boldog FL, MacDougall JR, Rastelli L, Herrmann J, Gallo M, Gazit-Bornstein G, Senter PD, Meyer DL, Lichenstein HS, LaRochelle WJ: CR011, a fully human monoclonal antibody-auristatin E conjugate, for the treatment of melanoma. Clin Cancer Res. 2006, 12 (4): 1373-1382. 10.1158/1078-0432.CCR-05-2018.View ArticlePubMedGoogle Scholar
- Chen YG, Wang Q, Lin SL, Chang CD, Chuang J, Ying SY: Activin signaling and its role in regulation of cell proliferation, apoptosis, and carcinogenesis. Exp Biol Med (Maywood). 2006, 231 (5): 534-544.Google Scholar
- Braun T, Arnold HH: The four human muscle regulatory helix-loop-helix proteins Myf3-Myf6 exhibit similar hetero-dimerization and DNA binding properties. Nucleic Acids Res. 1991, 19 (20): 5645-5651. 10.1093/nar/19.20.5645.View ArticlePubMedPubMed CentralGoogle Scholar
- Parker MH, Seale P, Rudnicki MA: Looking back to the embryo: defining transcriptional networks in adult myogenesis. Nat Rev Genet. 2003, 4 (7): 497-507. 10.1038/nrg1109.View ArticlePubMedGoogle Scholar
- Li J, Mayne R, Wu C: A novel muscle-specific beta 1 integrin binding protein (MIBP) that modulates myogenic differentiation. J Cell Biol. 1999, 147 (7): 1391-1398. 10.1083/jcb.147.7.1391.View ArticlePubMedPubMed CentralGoogle Scholar
- Li J, Rao H, Burkin D, Kaufman SJ, Wu C: The muscle integrin binding protein (MIBP) interacts with alpha7beta1 integrin and regulates cell adhesion and laminin matrix deposition. Dev Biol. 2003, 261 (1): 209-219. 10.1016/S0012-1606(03)00304-X.View ArticlePubMedGoogle Scholar
- Fougerousse F, Edom-Vovard F, Merkulova T, Ott MO, Durand M, Butler-Browne G, Keller A: The muscle-specific enolase is an early marker of human myogenesis. J Muscle Res Cell Motil. 2001, 22 (6): 535-544. 10.1023/A:1015008208007.View ArticlePubMedGoogle Scholar
- Semenza GL, Jiang BH, Leung SW, Passantino R, Concordet JP, Maire P, Giallongo A: Hypoxia response elements in the aldolase A, enolase 1, and lactate dehydrogenase A gene promoters contain essential binding sites for hypoxia-inducible factor 1. J Biol Chem. 1996, 271 (51): 32529-32537. 10.1074/jbc.271.51.32529.View ArticlePubMedGoogle Scholar
- Dai KS, Liew CC: A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. J Biol Chem. 2001, 276 (26): 23992-23999. 10.1074/jbc.M011208200.View ArticlePubMedGoogle Scholar
- Lecker SH: Ubiquitin-protein ligases in muscle wasting: multiple parallel pathways?. Curr Opin Clin Nutr Metab Care. 2003, 6 (3): 271-275. 10.1097/00075197-200305000-00001.PubMedGoogle Scholar
- McElhinny AS, Kazmierski ST, Labeit S, Gregorio CC: Nebulin: the nebulous, multifunctional giant of striated muscle. Trends Cardiovasc Med. 2003, 13 (5): 195-201. 10.1016/S1050-1738(03)00076-8.View ArticlePubMedGoogle Scholar
- Scanlan MJ, Raj BK, Calvo B, Garin-Chesa P, Sanz-Moncasi MP, Healey JH, Old LJ, Rettig WJ: Molecular cloning of fibroblast activation protein alpha, a member of the serine protease family selectively expressed in stromal fibroblasts of epithelial cancers. Proc Natl Acad Sci USA. 1994, 91 (12): 5657-5661. 10.1073/pnas.91.12.5657.View ArticlePubMedPubMed CentralGoogle Scholar
- Roll P, Rudolf G, Pereira S, Royer B, Scheffer IE, Massacrier A, Valenti MP, Roeckel-Trevisiol N, Jamali S, Beclin C, Seegmuller C, Metz-Lutz MN, Lemainque A, Delepine M, Caloustian C, de Saint Martin A, Bruneau N, Depetris D, Mattei MG, Flori E, Robaglia-Schlupp A, Levy N, Neubauer BA, Ravid R, Marescaux C, Berkovic SF, Hirsch E, Lathrop M, Cau P, Szepetowski P: SRPX2 mutations in disorders of language cortex and cognition. Hum Mol Genet. 2006, 15 (7): 1195-1207. 10.1093/hmg/ddl035.View ArticlePubMedGoogle Scholar
- Ruotsalainen H, Vanhatupa S, Tampio M, Sipila L, Valtavaara M, Myllyla R: Complete genomic structure of mouse lysyl hydroxylase 2 and lysyl hydroxylase 3/collagen glucosyltransferase. Matrix Biol. 2001, 20 (2): 137-146. 10.1016/S0945-053X(01)00130-5.View ArticlePubMedGoogle Scholar
- Mukai M, Torikata C, Iri H, Mikata A, Hanaoka H, Kato K, Kageyama K: Histogenesis of alveolar soft part sarcoma. An immunohistochemical and biochemical study. Am J Surg Pathol. 1986, 10 (3): 212-218. 10.1097/00000478-198603000-00008.View ArticlePubMedGoogle Scholar
- Miettinen M, Ekfors T: Alveolar soft part sarcoma. Immunohistochemical evidence for muscle cell differentiation. Am J Clin Pathol. 1990, 93 (1): 32-38.View ArticlePubMedGoogle Scholar
- Coira BM, Sachdev R, Moscovic E: Skeletal muscle markers in alveolar soft part sarcoma. Am J Clin Pathol. 1990, 94 (6): 799-800.View ArticlePubMedGoogle Scholar
- Rosai J, Dias P, Parham DM, Shapiro DN, Houghton P: MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. Am J Surg Pathol. 1991, 15 (10): 974-981.View ArticlePubMedGoogle Scholar
- Cullinane C, Thorner PS, Greenberg ML, Kwan Y, Kumar M, Squire J: Molecular genetic, cytogenetic, and immunohistochemical characterization of alveolar soft-part sarcoma. Implications for cell of origin. Cancer. 1992, 70 (10): 2444-2450. 10.1002/1097-0142(19921115)70:10<2444::AID-CNCR2820701010>3.0.CO;2-6.View ArticlePubMedGoogle Scholar
- Tallini G, Parham DM, Dias P, Cordon-Cardo C, Houghton PJ, Rosai J: Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. Am J Pathol. 1994, 144 (4): 693-701.PubMedPubMed CentralGoogle Scholar
- Cessna MH, Zhou H, Perkins SL, Tripp SR, Layfield L, Daines C, Coffin CM: Are myogenin and myoD1 expression specific for rhabdomyosarcoma? A study of 150 cases, with emphasis on spindle cell mimics. Am J Surg Pathol. 2001, 25 (9): 1150-1157. 10.1097/00000478-200109000-00005.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/9/22/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.