Figure 7

Effect of H-8 on MUC2 and NF-Kb p65 expression. (a) SEG-1 cells were treated for 18 hours with or without 100 μM DCA in the presence or absence of 5 μM H-8. Total cellular protein and nuclear protein were subjected to Western blotting for MUC2, NF-κB p65, and β-actin. (b) SEG-1 cells were transfected with the MUC2 promoter luciferase construct or the NF-κB luciferase reporter plasmid, then treated for 18 hours with or without 100 μM DCA in the presence or absence of 5 μM H-8. Luciferase activity for MUC2 or NF-κB was measured and normalized to beta-galactosidase activity. Values shown represent the mean and standard error of the mean of triplicate experiments.