Figure 3

Effect of CAPE on expression of MUC2. SEG-1 cells were pretreated with or without 10 μg/ml CAPE for 1 h and then treated for 18 hours with or without 100 μM DCA. (a) Total cellular protein was subjected to Western blotting for MUC2 and β-actin. (b) The isolated RNA samples were analyzed by RT-PCR for MUC2, NF-κB p65 and β-actin. (c) SEG-1 cells were transfected with MUC2 promoter luciferase construct, then treated for 18 hours with or without 100 μM DCA in the presence or absence of CAPE. Luciferase activity for MUC2 was measured and normalized to beta-galactosidase activity. (means ± SD of triplicate assays, * p < 0.05, for inhibition compared with assays without added inhibitor).