Figure 2

Phenotypic characterisation of induced Cre+Apc ^fl/fl p53 ^-/- and Cre+Apc ^fl/fl p53 ^+/+ intestinal samples. The top panel represents induced Cre+Apc ^fl/fl p53 ^+/+ intestinal samples, while the bottom panel represents induced Cre+Apc ^fl/fl p53 ^-/- intestinal samples. a+b) H&E staining displays no ascertainable difference between (a) the induced Cre+Apc ^fl/fl p53 ^+/+ and (b) the induced Cre+Apc ^fl/fl p53 ^-/-. c+d) β-catenin IHC and e+f) cMyc IHC display nuclear staining throughout the aberrant structure in both sample types. g+h) IHC detecting lysozyme positivity shows that paneth cell mis-localisation occurs in an identical manner in both genotypes with complete mis-localisation occurring 6 days after induction. i+j) Grimelius staining shows no ascertainable difference between the two induced genotypes. k+l) p21CIP1/WAF1 IHC displays a band of positively stained cells displaying an enlarged nuclear area and the leading edge of aberrancy adjacent to phenotypically normal cells in both induced genotypes. m+n) γ H2AX IHC shows no difference between the 2 genotypes. p) Apoptotic and mitotic crypt indices show no differences between the induced Cre+Apc ^fl/fl p53 ^-/- and Cre+Apc ^fl/fl p53 ^+/+ intestinal samples (assessed using t-test), asterisks signify a significant difference (p < 0.05) compared to the control levels. q) Characterisation of the nuclear area at the crypt-villus (or aberrant-normal) junction show both the induced Cre+Apc ^fl/fl p53 ^-/- and Cre+Apc ^fl/fl p53 ^+/+ samples display an increase in the number of larger nuclei compared to control Cre+Apc ^+/+ p53 ^-/- and Cre+Apc ^+/+ p53 ^+/+ samples. Yet again no differences between the induced Cre+Apc ^fl/fl p53 ^-/- and Cre+Apc ^fl/fl p53 ^+/+ samples could be identified.