Cellular morphology of serous ovarian epithelium cancer cell lines and their corresponding tumoral tissues. A, C, E) Morphological appearance of the TOV-1946, OV-1946 and TOV-2223 cell lines respectively at passage 0. More (TOV-1946 and TOV-2223) or less (OV-1946) heterogenous populations of cells with obvious fibroblastic contaminants are visible for all three cell lines. B,D,F) Appearance of TOV-1946, OV-1946 and TOV-2223 cell lines respectively at passage 70. An evolution toward a more homogenous population of cobblestone-like cells typical of an epithelial cell type. G,H) Hematoxylin-Eosin stained tumor tissue sections of patient 1946 and 2223. Poorly differentiated tumor masses can be observed. All primary cultures and cell lines were cultured in OSE medium composed of 50:50 medium 199:105 (Sigma) supplemented with 10% fetal bovine serum (FBS), 2.5 μg/mL amphotericin B and 50 μg/mL gentamicin. All photographs were taken at 400× magnification.