Table 1 Primers for PCR experiments
From: Expression of TRPC6 channels in human epithelial breast cancer cells
Gene | Accession n° | Primer | Sequence (5-3) | Predicted Size, bp |
|---|---|---|---|---|
hTRPC3 | NM003305 | Sense | GGAAAAACATTACCTCCACCTTTCA | Â |
| Â | Â | Antisense | CTCAGTTGCTTGGCTCTTGTCTTCC | 383 pb |
hTRPC6 | NM 004621 | Sense | GAACTTAGCAATGAACTGGCAGT | 625 pb for TRPC6 |
|  |  | Antisense | CATATCATGCCTATTACCCAGGA | 277 pb for TRPC6γ |
hTRPC7 | NM 020389 | Sense | GTCCGAATGCAAGGAAATCT | Â |
| Â | Â | Antisense | TGGGTTGTATTTGGCACCTC | 477 pb |
hβ-actin | NM 001101 | Sense | CAGAGCAAGAGAGGCATCCT |  |
| Â | Â | Antisense | ACGTACATGGCTGGGGTG | 210 pb |
hCK19 | NM 002276 | Sense | GATTGCCACCTACCGC | Â |
| Â | Â | Antisense | CCATCCCTCTACCCAG | 136 pb |