Figure 6

Apoptosis or necrosis: Annexin-V-FITC /PI double staining of HeLa cells after treatment with CSN kinase inhibitors. HeLa cells were cultured in medium containing 0.1% DMSO or the curcumin- and emodin-derived inhibitors at different concentrations (20 μM and 50 μM). After 24 h cell death was measured at the single-cell level by labeling cells with annexin-V-FITC and counterstaining with propidium iodide (PI). (A) Representative data for piceatannol and BTB14431 are shown. The numbers indicate the percentage of cells in each quadrant (lower left: FITC^-/PI^-, intact cells; lower right: FITC⁺/PI^-, apoptotic cells; upper left: FITC^-/PI⁺, necrotic cells; upper right: FITC⁺/PI⁺, late apoptotic or necrotic cells). (B) The bar chart describes the percentual distribution of necrotic, apoptotic and viable cells after treatment with curcumin, emodin, resveratrol, piceatannol or BTB14431.