- Case report
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Gallbladder adenocarcinoma with sarcoid-like reaction in regional lymph nodes: report of a case
BMC Cancer volume 14, Article number: 946 (2014)
Sarcoid-like reaction is often seen in various types of carcinoma, not only in the primary tumor, but also in regional lymph nodes, and can occur at any time, not only at the time of diagnosis, but also after treatment. However, few cases of hepatopancreatobiliary carcinoma, and no cases of gallbladder cancer with sarcoid-like reaction involving the lymph nodes have been described. This report is the first report of a sarcoid-like reaction involving the lymph nodes in a case of gallbladder cancer.
We encountered a rare case of gall bladder cancer with sarcoid-like reaction in the lymph nodes. Since regional lymph node swelling that was difficult to differentiate from metastasis was found preoperatively, swollen nodes were examined histologically using frozen sections. Based on this histology, the swollen nodes were diagnosed as showing sarcoid reaction and therefore extended lymphadenectomy was avoided. The patient did not receive any adjuvant chemotherapy and has shown no recurrence of disease as of 4 years after surgery.
Distinguishing between metastasis and sarcoid-like reaction in lymph nodes by preoperative imaging is still difficult. The present case shows that it is important to histologically examine swollen nodes by biopsy or by sampling before deciding on the treatment strategy for gall bladder cancer with swollen lymph nodes.
Sarcoid-like reaction is known to occur in patients with malignant disease and can occur at any time, not only at the time of diagnosis, but also after treatment . Sarcoid-like reaction can be found in the stroma of the primary tumor itself, in lymph nodes draining the area of the primary tumor, and even in organs such as the liver, spleen, stomach and lungs [1–3].
Pathologically, sarcoid-like reaction includes non-caseating epithelioid cell granuloma that is comprised of a focal accumulation of epithelioid cells and multinucleated giant cells, and patients with sarcoid-like reaction show no clinical symptoms of systemic sarcoidosis. The non-caseating epithelioid granuloma is a result of antigen-specific cell-mediated immunity caused by macrophages and T-lymphocytes . Although the pathogenesis of sarcoid-like reaction has been reported, no consensus has been reached on a complete definition. Sarcoid-like reaction is hypothesized to represent an antineoplastic immune phenomenon caused by antigenic factors derived from the tumor, with the high reactivity of the host immune system against such antigenic factors leading to the development of non-caseating epithelioid granuloma [2, 5].
Although many authors have reported sarcoid-like reactions in cancer patients, this finding appears to be very rare in patients with hepatopancreatobiliary cancer . We report herein a rare case of gall bladder cancer with sarcoid reaction in the lymph nodes and no systemic sarcoid phenomenon.
A 73-year-old Japanese woman was referred to our institution after a gall bladder mass was detected on screening ultrasonography (US).The patient had no complaints, and both physical examination and laboratory findings (including carcinoembryonic antigen, carbohydrate antigen 19–9, and soluble interleukin-2 receptor) were unremarkable. When we performed US, a mass measuring 23 × 22 mm was identified at the fundus of the gall bladder. This broad-based, sessile mass showed preservation of the outer hyperechoic layer, suggesting gall bladder cancer. According to a previous report, these findings corresponded with a Type B image . Tumor invasion seemed limited to the muscularis propria (cT1b) . Computed tomography (CT) revealed several lymph nodes that were swollen to 7–15 mm, located at both the hepatoduodenal ligament and in the para-aortic area. Diffusion-weighted imaging (DWI) showed a signal-hyperintense gallbladder mass, but signal hypointensity for all detected swollen lymph nodes (Figure 1). Based on such findings, the swollen nodes were diagnosed as metastases. As a preoperative summary, we regarded this tumor as clinically stage IIB (cT1b, cN1, cM0) based on the 7th edition of the Union for International Cancer Control (UICC).
Based on the above evidence of gallbladder cancer, we planned to perform radical cholecystectomy involving removal of the gallbladder and extrahepatic biliary tract with en-bloc subsegmental resection of the adjacent hepatic parenchyma of segments 4B and 5, and regional lymphadenectomy involving complete removal of the hepatoduodenal ligament lymph nodes, common hepatic artery nodes and retropancreatic nodes . During surgery, we detected swollen lymph nodes. Four of these lymph nodes were dissected and frozen sections were histologically examined. Unlike typical metastatic lymph nodes, these nodes showed a smooth, round shape with clear margins, and were easy to dissect (Figure 2). Histological frozen-section examination revealed that none of the lymph nodes showed tumor involvement but instead they showed non-caseating epithelioid cell granuloma (Figure 2). We therefore only performed simple cholecystectomy, and some of the swollen nodes in the hepatoduodenal ligament were not dissected. Histological examination of the resected gall bladder showed papillary carcinoma with invasion limited to the muscularis propria. None of the dissected lymph nodes showed tumor involvement and all were diagnosed as showing non-caseating epithelioid cell granuloma. Accordingly, the patient was diagnosed with papillary adenocarcinoma of the gall bladder, stage IA (pT1b, pN0, pM0) (Figure 3). Because of the early stage of gall bladder cancer, adjuvant chemotherapy was not performed. As of 4 years postoperatively, the patient has shown no disease recurrence. During follow-up examination, the size of swollen nodes remaining in the hepatoduodenal ligament has gradually decreased compared to the initial size at the time of surgery (Figure 4).
Sarcoid-like reaction related to a tumor was first reported in the early 19th Century . Since then, such reactions have been reported with various carcinomas, such as those of the stomach, lungs, uterus and breast [3, 9, 10]. Brincker et al.  reported that sarcoid-like reactions may occur in 4.4% of cancer patients, 13.8% of patients with Hodgkin’s disease, and 7.3% of patients with non-Hodgkin lymphoma. In cases of carcinoma, sarcoid-like reaction is found in regional lymph nodes, particularly in non-metastatic lymph nodes. Few cases of hepatopancreatobiliary carcinoma with sarcoid-like reaction involving the lymph nodes have been described. Fong et al.  summarized 15 cases of hepatopancreatobiliary tumor with sarcoid-like reaction in 2012. However, only 19 cases in total have been reported to date (Table 1), and there have been no reports regarding sarcoid-like reaction in gallbladder cancer.
Gallbladder cancer is the most common malignant neoplasm of biliary tract cancer, and unfortunately the prognosis is well known to be very poor . In many cases, gall bladder cancer is already at an advanced stage by the time of detection and diagnosis . Lymph node metastasis is an important prognostic factor for this tumor, as it is for other malignant tumors. Preoperative diagnosis of the presence or absence of nodal metastasis is thus important when planning a surgical strategy. In the case of node positive gallbladder cancer (except for positive para-aortic lymph nodes), although there is certainly some controversy, it is considered that “aggressive” radical surgery such as combined resection of the liver and the common bile duct together with extended lymphadenectomy might contribute to better prognosis [26, 27]. In the present case, we preoperatively diagnosed multiple nodal swellings observed by imaging as positive evidence of metastasis. Distinct radiographic patterns that can distinguish sarcoid-like reaction, sarcoidosis and carcinoma have not yet been identified . If the differential diagnosis of lymph node swelling is difficult, intraoperative histological examination of frozen sections is essential. In this case, we were able to avoid extended lymphadenectomy, because the histological results showed a sarcoid-like reaction. Some authors have reported that preoperative biopsy through endoscopic ultrasound-guided-fine needle aspiration (EUS-FNA) may represent a useful method for analysis of such nodes [1, 28, 29].
The medical records of this patient included no symptoms or signs related to systemic sarcoidosis, such as erythema nodosum, uveitis, acute polyarthritis, respiratory illness, fatigue, malaise, weight loss or fever . Moreover, no serum abnormalities such as hypercalcemia or elevated levels of angiotensin-converting enzyme were seen. Accordingly, we speculate that the development of non-caseating epithelioid cell granuloma in this patient was not caused by systemic sarcoidosis, but rather by a sarcoid-like reaction related to gallbladder cancer. Sarcoid-like reactions are comprised of different inflammatory cells, mature dendritic cells and T lymphocytes in non-caseating epithelioid cell granulomas . Infiltration of tumors by dendritic cells reflects a host immune defense mechanism . It is well known that the presence of dendritic cells and T lymphocytes in tumors is associated with better prognosis, reduced tumor recurrence and fewer metastases [32–36]. Although no consensus has been reached, these evidences seem to suggest that patients with a malignant tumor that shows a sarcoid-like reaction may have a better prognosis . Indeed, our patient has shown long-term survival and no recurrence after surgery.
Few reports have focused on the course of swollen nodes after resection of a primary cancer. Interestingly, in this case, the residual swollen nodes gradually decreased in size after resection of the gallbladder cancer. This phenomenon clinically supports the view that sarcoid-like reaction results from high reactivity of the host immune system against antigenic factors from the primary cancer.
We encountered a case of sarcoid-like reaction related to gallbladder cancer. Distinguishing between metastasis and sarcoid-like reaction in lymph nodes by preoperative imaging is still difficult. The present case shows that it is important to histologically examine swollen nodes by biopsy or sampling before deciding on the treatment strategy for gall bladder cancer with swollen lymph nodes.
Written informed consent was obtained from the patient for publication of this Case report and any accompanying images. A copy of this written consent is available for review by the Editor-in-Chief of this journal. This study was approved by the institutional ethics review board of Kagoshima University Hospital (reference number 25–39).
Computed tomography CT
Diffusion-weighted imaging DWI
Union for International Cancer Control
Endoscopic ultrasound-guided-fine needle aspiration.
Craun JB, Banks KP, Clemenshaw MN, Moren RW: Sarcoidlike reaction of neoplasia causing hypermetabolic thoracic adenopathy in setting of extrathoracic malignancy: report of two cases and a review of the differential diagnostic considerations. J Nucl Med Technol. 2012, 40 (4): 231-235. 10.2967/jnmt.112.102814.
Chowdhury FU, Sheerin F, Bradley KM, Gleeson FV: Sarcoid-like reaction to malignancy on whole-body integrated (18)F-FDG PET/CT: prevalence and disease pattern. Clin Radiol. 2009, 64 (7): 675-681. 10.1016/j.crad.2009.03.005.
Murata Y, Tachibana T: Sarcoid reactions in malignant tumor. Kekkaku: Tuberculosis. 1979, 54 (10): 510-511.
Sugiyama Y, Oshikawa K: Mechanism of sarcoid granuloma formation–participation of cytokines and chemokines. Nihon Rinsho. 2002, 60 (9): 1728-1733.
Brincker H: Sarcoid reactions in malignant tumours. Cancer Treat Rev. 1986, 13 (3): 147-156. 10.1016/0305-7372(86)90002-2.
Fong ZV, Wong J, Maley WR, Sandorfi N, Winter JM, Koniaris LG, Sauter PK, Yeo CJ, Lavu H: Sarcoid-reaction mimicking metastatic malignant hepatopancreatobiliary tumors: report of two cases and review of the literature. J Gastrointest Surg. 2012, 16 (6): 1245-1250. 10.1007/s11605-011-1820-3.
Sadamoto Y, Kubo H, Harada N, Tanaka M, Eguchi T, Nawata H: Preoperative diagnosis and staging of gallbladder carcinoma by EUS. Gastrointest Endosc. 2003, 58 (4): 536-541. 10.1067/S0016-5107(03)01961-8.
Reid KM, Ramos-De la Medina A, Donohue JH: Diagnosis and surgical management of gallbladder cancer: a review. J Gastroint Surg. 2007, 11 (5): 671-681. 10.1007/s11605-006-0075-x.
Gregorie HB, Othersen HB, Moore MP: The significance of sarcoid-like lesions in association with malignant neoplasms. Am J Surg. 1962, 104: 577-586. 10.1016/0002-9610(62)90399-9.
Kamiyoshihara M, Hirai T, Kawashima O, Ishikawa S, Morishita Y: Sarcoid reactions in primary pulmonary carcinoma: report of seven cases. Oncol Rep. 1998, 5 (1): 177-180.
Herxheimer G: Uber Karzinom und Tuberkulose. Z Tuberk. 1917, 27: 251-
Gherardi G: Localized lymph node sarcoidosis associated with carcinoma of the bile ducts; report of a case. AMA Arch Pathol. 1950, 49 (2): 163-168.
Nadel E: Lesions resembling Boeck’s sarcoid in lymph nodes draining an area containing a malignant neoplasm. Am J Clin Pathol. 1950, 20 (10): 952-957.
Ten Seldam R: Sarcoid-like lesions in lymph nodes draining carcinoma. Med Aust. 1956, 43 (22): 916-919.
Muto Y, Uchimira M, Waki S, Hayashi T, Sameshima Y, Okamoto K: A case of common duct carcinoma with sarcoid reaction in the regional lymph nodes. Jpn J Gastroenterol Surg. 1982, 15: 512-515. 10.5833/jjgs.15.512.
Schmidt D, Harms D, Lang W: Primary malignant hepatic tumours in childhood. Virchows Arch A Pathol Anat Histopathol. 1985, 407 (4): 387-405. 10.1007/BF00709986.
Van Steenbergen W, Fevery J, Vandenbrande P, Desmet V, Ponette E, Kerremans R, de Groote J: Ulcerative colitis, primary sclerosing cholangitis, bile duct carcinoma, and generalized sarcoidosis. Report of a unique association. J Clin Gastroenterol. 1987, 9 (5): 574-579. 10.1097/00004836-198710000-00020.
Klein M, Kaminsky P, Deibener J, Cocciale MP, Duc M: Sarcoid reactions in cystic duct carcinoma. Chest. 1994, 106 (4): 1304-1305. 10.1378/chest.106.4.1304.
Nakao A, Sato Y, Nakashima A: A case of bile duct cancer with sarcoid reaction in the regional lymph nodes. Biliary Tract Pancreas. 1996, 17: 963-966.
Shito M, Shintoku J, Miyazaki H: Primary caricnoma of the cystic duct associated with sarcoid reactions: report of a case. Surg Today. 1997, 27: 1177-1181. 10.1007/BF02385690.
Onitsuka A, Katagiri Y, Kiyama S, Mimoto H, Nakamura T, Toda K, Shima H: Hilar cholangiocarcinoma associated with sarcoid reaction in the regional lymph nodes. J Hepatobiliary Pancreat Surg. 2003, 10 (4): 316-320. 10.1007/s00534-002-0817-4.
Mao JT, Fisnbein MC: Metastatic pancreatic cancer masquerading as pulmonary sarcoidosis. Am J Med. 2000, 109 (7): 598-599. 10.1016/S0002-9343(00)00537-4.
Kurata A, Terado Y, Schulz A, Fujioka Y, Franke FE: Inflammatory cells in the formation of tumor-related sarcoid reactions. Hum Pathol. 2005, 36 (5): 546-554. 10.1016/j.humpath.2005.02.017.
Mastroroberto M, Berardi S, Fraticelli L, Pianta P, Ercolani G, Cancellieri A, Sama C: Sarcoidosis and sarcoid-like reaction associated with pancreatic malignancy: are you able to read a riddle?. JOP. 2012, 13 (4): 454-457.
Carriaga MT, Henson DE: Liver, gallbladder, extrahepatic bile ducts, and pancreas. Cancer. 1995, 75 (1 Suppl): 171-190.
Foster JM, Hoshi H, Gibbs JF, Iyer R, Javle M, Chu Q, Kuvshinoff B: Gallbladder cancer: defining the indications for primary radical resection and radical re-resection. Ann Surg Oncol. 2007, 14 (2): 833-840. 10.1245/s10434-006-9097-6.
Kondo S, Nimura Y, Hayakawa N, Kamiya J, Nagino M, Uesaka K: Regional and para-aortic lymphadenectomy in radical surgery for advanced gallbladder carcinoma. Br J Surg. 2000, 87 (4): 418-422. 10.1046/j.1365-2168.2000.01384.x.
Wallace MB, Kennedy T, Durkalski V, Eloubeidi MA, Etamad R, Matsuda K, Lewin D, Van Velse A, Hennesey W, Hawes RH, Hoffman BJ: Randomized controlled trial of EUS-guided fine needle aspiration techniques for the detection of malignant lymphadenopathy. Gastrointest Endosc. 2001, 54 (4): 441-447. 10.1067/mge.2001.117764.
Williams DB, Sahai AV, Aabakken L, Penman ID, van Velse A, Webb J, Wilson M, Hoffman BJ, Hawes RH: Endoscopic ultrasound guided fine needle aspiration biopsy: a large single centre experience. Gut. 1999, 44 (5): 720-726. 10.1136/gut.44.5.720.
Hunsaker AR, Munden RF, Pugatch RD, Mentzer SJ: Sarcoidlike reaction in patients with malignancy. Radiology. 1996, 200 (1): 255-261. 10.1148/radiology.200.1.8657922.
Banchereau J, Steinman RM: Dendritic cells and the control of immunity. Nature. 1998, 392 (6673): 245-252. 10.1038/32588.
Zhang L, Conejo-Garcia JR, Katsaros D, Gimotty PA, Massobrio M, Regnani G, Makrigiannakis A, Gray H, Schlienger K, Liebman MN, Rubin SC, Coukos G: Intratumoral T cells, recurrence, and survival in epithelial ovarian cancer. N Engl J Med. 2003, 348 (3): 203-213. 10.1056/NEJMoa020177.
Tsujitani S, Kakeji Y, Watanabe A, Kohnoe S, Maehara Y, Sugimaghi K: Infiltration of S-100 protein positive dendritic cells and peritoneal recurrence in advanced gastric cancer. Int Surg. 1992, 77 (4): 238-241.
Iwamoto M, Shinohara H, Miyamoto A, Okuzawa M, Mabuchi H, Nohara T, Gon G, Toyoda M, Tanigawa N: Prognostic value of tumor-infiltrating dendritic cells expressing CD83 in human breast carcinomas. Int J Cancer. 2003, 104 (1): 92-97. 10.1002/ijc.10915.
Ishigami S, Aikou T, Natsugoe S, Hokita S, Iwashige H, Tokushige M, Sonoda S: Prognostic value of HLA-DR expression and dendritic cell infiltration in gastric cancer. Oncology. 1998, 55 (1): 65-69. 10.1159/000011837.
Inoue K, Furihata M, Ohtsuki Y, Fujita Y: Distribution of S-100 protein-positive dendritic cells and expression of HLA-DR antigen in transitional cell carcinoma of the urinary bladder in relation to tumour progression and prognosis. Virchows Arch A Pathol Anat Histopathol. 1993, 422 (5): 351-355. 10.1007/BF01605452.
Yamamoto T, Tateishi H, Nishimura Y, Watanabe M, Ukyo S, Miyake T, Uchino H, Matsumoto Y, Ishii K, Takahashi K: A study of gastric cancer with sarcoid reaction as observed in the regional lymph node. Nihon Shokakibyo Gakkai Zasshi. 1980, 77 (10): 1555-1561.
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We declare that each author received no funding for this study.
The authors declare that they have no competing interests.
YK performed the surgery, participated in the conception, coordinated with other doctors about the design of the study, collected data and a consent, carried out the histological evaluation and drafted the manuscript. KM participated in the surgery, histological evaluation and in drafting the manuscript. HK, YM and HS participated in the surgery and histological evaluation. SI, MS, SU, ST and SN conceived of the study, participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
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Kawasaki, Y., Maemura, K., Kurahara, H. et al. Gallbladder adenocarcinoma with sarcoid-like reaction in regional lymph nodes: report of a case. BMC Cancer 14, 946 (2014). https://doi.org/10.1186/1471-2407-14-946
- Sarcoid-like reaction
- Gallbladder cancer
- Multiple swollen lymph nodes