Figure 7

LEIGC inhibited EMT in MGC-803 cells. (A). MGC-803 cells transfected with blank control vector (blank); (B and C). Cells transfected with LEIGC-shRNA vector (shRNA) and scrambled shRNA vector (NC1); (D and E). Cells transfected with LEIGC vector (lncRNA) and non-related lncRNA vector (NC2). (F). Effects of LEIGC on the expression of EMT-related genes CDH1, twist, snail, slug, and zeb at the mRNA level were analyzed by qRT- PCR. *P<0.05. (G). Effects of LEIGC on the expression of EMT-related proteins. After transfection of the cells with different vectors, E-cadherin, Vimentin, Twist, Slug, and Zeb expression levels were determined by western blotting. GAPDH protein levels served as an internal control.