Number of tumor foci predicts prognosis in papillary thyroid cancer
- Ning Qu†1,
- Ling Zhang†1,
- Qing-hai Ji1Email author,
- Yong-xue Zhu1,
- Zhuo-ying Wang1,
- Qiang Shen1,
- Yu Wang1 and
- Duan-shu Li1
© Qu et al.; licensee BioMed Central Ltd. 2014
Received: 14 July 2014
Accepted: 26 November 2014
Published: 4 December 2014
Papillary thyroid cancer (PTC) often presents as multifocal. However, the association of multifocality with poor prognosis remains controversial. The aim of this retrospective study was to identify the characteristics of PTC with multiple foci and to evaluate the association between multifocality and prognosis.
We reviewed the medical records of 496 patients who underwent total thyroidectomy for PTC. Patients were classified as G1 (1 tumor focus), G2 (2 foci), and G3 (3 or more foci). We analyzed the clinicopathological features and clinical outcomes in each classification. A Cox regression model was used to assess the relationship between multifocality and recurrence or cancer mortality.
The G1, G2 and G3 groups included 287, 141 and 68 patients, respectively. The mean age was 47.1 ± 16.1 yr in G1, 41.1 ± 18.4 yr in G2, and 35.5 ± 15.9 yr in G3 and differed significantly among the 3 groups (p = 0.001). The proportion of extrathyroidal extension, central lymph node metastasis (CLNM), and lateral lymph node metastasis (LLNM) in the G1 to G3 groups increased with increasing number of tumor foci. The Kaplan–Meier curves revealed that G3 had the shortest recurrence-free survival, and differences were significant among the 3 groups (p = 0.001, Log Rank test). Furthermore, cancer-specific survival rates decreased significantly with increasing number of tumor foci (p = 0.041). Independent predictors of recurrence by multivariate Cox analysis included >3 tumor foci [HR 2.60, 95% confidence interval (CI) 1.53-4.39, p = 0.001] and extrathyroidal extension (HR 1.95, CI 1.12-3.38, p = 0.018).
An increase in the number of tumors is associated with a tendency toward more aggressive features and predicts poor prognosis in PTC.
KeywordsPapillary thyroid carcinoma Multifocality Recurrence Mortality
The number of newly diagnosed thyroid carcinoma cases is increasing annually, and an estimated 62,980 cases will be diagnosed in the United States in 2014, more than 90% of which will be papillary thyroid cancer (PTC) [1, 2]. PTC often presents as multifocal tumors , which are thought to arise independently, indicating an inherent propensity to develop PTC, and spread throughout the thyroid gland .
PTC is generally an indolent disease and has a favorable prognosis in most affected patients; however, PTC with multifocal foci is likely to be aggressive and, accordingly, require aggressive treatment [5, 6]. The characteristics of PTC with multifocal foci and the prognostic significance of multifocality in PTC remain controversial [7, 8]. We performed a retrospective analysis to examine the characteristics of multifocal PTC and the relationship between the number of tumor foci and PTC prognosis in a large group of Chinese patients with long-term follow-up.
The records of all PTC patients treated at our center between January 1, 1983, and December 31, 2007, were retrospectively reviewed. All patients provided written informed consent for their information to be stored in the hospital database and used for research, and this study was approved by the Ethical Committee of the Cancer Center of Fudan University. A series of 496 consecutive PTC patients who underwent primary surgical therapy of total thyroidectomy (TT) were enrolled in this study, representing 23.5% of the total 2115 patients treated at our center during the corresponding period.
Before surgery, each patient underwent an ultrasonography (US) examination. US-guided fine-needle aspiration (FNA) was not performed routinely. Lobectomy plus ipsilateral central lymph node dissection (CLND) was typically performed as the initial surgical treatment for PTC patients with malignant lesions that were limited to a single lobe . When the patient was older than 45 yr, the primary tumor was greater than 1 cm, undetermined nodules were detected in the contralateral lobe by US or regional metastases or multifocal tumors were present, TT was performed at the time of the initial operation. When undetermined nodules were detected in the contralateral lobe by US without other factors, a subtotal lobectomy of approximately one-fourth to two-thirds of the contralateral lobe was performed on the suspicion of lesions in the contralateral lobe following the preoperative US. Histology of the frozen sections (FS) assisted surgeons in determining the extent of the surgical procedures. When malignant lesions were identified in both lobes of the thyroid by FS after a subtotal lobectomy, a completion thyroidectomy (CT) was performed . Modified lateral lymph node dissection (LLND), including levels II–V, was performed in cases with pathologically proven lymph node metastasis (LNM) or suspicious lymph nodes observed intraoperatively or on preoperative imaging.
Suppressive treatment with thyroid hormone was initiated after surgery to decrease serum thyroid-stimulating hormone (TSH) to subnormal levels without clinical thyrotoxicosis. Because its use is strictly controlled in China, radioactive iodine (RAI) therapy was not routinely prescribed for PTC patients after surgery unless patients had distant metastases .
Clinicopathological variable assessment and statistical analysis
Multifocality was defined as 2 or more tumor foci within the thyroid. For multifocal tumors, the diameter of the largest tumor focus was taken as the primary tumor. In addition, data on patient clinical features (gender, age at diagnosis), tumor histological characteristics (maximum size of tumor, extrathyroidal extension), and cervical LNM were extracted from the database.
All patients gave consent to participate in the follow-up study. The follow-up period for each patient was defined as the length of time from the initial therapy until the last known date of contact documented by a review of the medical record or as a follow-up phone call to the patient. Postsurgical physical examinations were performed every 3–6 months. During the follow-up visits, all patients underwent US examinations of the neck. Recurrence was defined as the appearance of disease, including new biopsy-proven/secondary surgery-confirmed local disease or distant disease revealed by ultrasonography and/or imaging scans, in any patient who had been free of disease (i.e., no palpable disease and negative radioactive iodine scan). New biopsy-proven/secondary surgery-confirmed local disease within the residual thyroid gland or lateral lymph nodes was classified as neck recurrence; distant disease revealed by ultrasonography and/or imaging scans of other sites, including the lungs, bones or brain, was classified as distant recurrence.
The results are expressed as the mean ± standard deviation. Statistical analysis was performed using Student’s t test, Χ2 test or Mann–Whitney test as appropriate. Patients who were alive and who did not relapse were censored at the date of their last follow-up visit. Neck/metastasis recurrence-free survival (RFS) was defined as the time between the date of initial surgery and the first event of recurrence or death. Overall survival (OS) was defined as the time between the date of initial surgery and death (all causes or cancer-specific). Cancer-specific survival (CSS) was defined as the time between the date of initial surgery and cancer-specific death. Survival rates were estimated using the Kaplan-Meier method . The hazard ratio (HR) and the 95% confidence interval (CI) for relationships between each variable and recurrence were calculated using a binary Cox regression model . A p < 0.05 was considered statistically significant. Statistical analyses were performed using SPSS for Windows 13.0 (SPSS Inc., Chicago, IL).
Baseline characteristics of patients
Clinicopathological characteristics of 496 papillary thyroid cancer patients
43.8 ± 17.3 yr
Maximum size of tumor (cm)
2.31 ± 1.59
Number of tumor foci
1.2 ± 1.3
Central lymph node metastasis
Lateral lymph node metastasis
Comparison among groups according to the number of tumor foci
Clinicopathological features of papillary thyroid cancer patients according to the number of tumor Foci
G1 (n = 287)
G2 (n = 141)
G3 (n = 68)
47.1 ± 16.1 yr
41.1 ± 18.4 yr
35.5 ± 15.9 yr
Maximum size of tumor (cm)
2.29 ± 1.41
2.35 ± 1.9
2.37 ± 1.60
Central lymph node metastasis
Lateral lymph node metastasis
Died of thyroid cancer
The mean age was 47.1 ± 16.1 yr in G1, 41.1 ± 18.4 yr in G2, and 35.5 ± 15.9 yr in G3 and differed significantly among the 3 groups (p = 0.001). A decreasing trend of age was observed from G1 to G3 according to the increasing number of tumor foci.
The incidence of neck recurrence was highest in group G3, followed by group G2 and group G1, and differed significantly among the 3 groups (p = 0.037). However, there were no significant differences with respect to gender, maximum tumor size, extrathyroidal extension, CLNM, LLNM, distant recurrence or cancer-specific death among the 3 groups. However, the proportion of aggressive features, such as extrathyroidal extension, CLNM, and LLNM, in the G1 to G3 groups exhibited an increasing trend according to the number of tumor foci.
Number of tumor foci and recurrence
Multivariate cox regression for recurrence
HR (95% CI)
Gender (female vs. male)
1.95 (1.12 -3.38)
Number of tumor foci
G1 (1 tumor focus)
G2 (2 foci)
G3 (3 or more foci)
Central lymph node metastasis
Lateral lymph node metastasis
Number of tumor foci and survival
Of the 496 patients, 26 (5.2%) had transient hypoparathyroidism, and 6 (1.2%) had permanent hypoparathyroidism. Recurrent laryngeal nerve injury occurred in 11 patients (2.2%), of which 2 were transient (0.4%) and 9 were permanent (1.8%).
In this study, we investigated the relationship between the number of tumor foci and other clinicopathological features and the impact of multifocality on prognosis in PTC. We observed that an increasing number of tumor foci was associated with both a tendency toward more aggressive features as well as poor prognosis in PTC.
Few studies have addressed the associations between the number of tumor foci and clinicopathological features in multifocal PTCs. Kim et al.  reported that an increase in the number of tumor foci was strongly associated with older age at diagnosis, cervical LNM, and advanced TNM stage of PTC; furthermore, the number of tumor foci independently predicted LNM. In accordance with their findings, we observed that an increasing number of tumor foci was associated with a tendency toward more aggressive features, such as larger primary tumor size, more frequent extrathyroidal extension and cervical LNM. Although these differences were too small to be significant, they suggest that multifocality might represent the tumor burden and predict more aggressive behavior during disease progression. An interesting finding of the current study was that an increasing number of tumor foci was associated with a younger age at diagnosis in PTC, in contrast to previous reports that advanced age is a prognostic factor for poor prognosis in PTC [12–15]. Younger age may indicate greater risk for the evolution of biological aggressiveness, such as multifocality; we previously reported that younger age is significantly associated with cervical LNM in PTC. However, appropriate initial management may improve the prognosis of younger PTC patients. Younger age as a predictor of multifocality emphasizes the need for more aggressive therapy to achieve the relatively preferable outcomes observed in elderly patients.
Recent studies of the relationship between multifocality and oncological outcomes have suggested that multifocal lesions in PTC are positively associated with poorer prognosis in patients. Kim et al.  reviewed the medical records of 2095 patients who underwent total thyroidectomy for PTC and reported that multifocality is associated with an increased risk of disease recurrence and persistence, suggesting that the number of tumor foci is a significant predictor of poor clinical outcomes. We determined that multifocality is a risk factor for poor outcomes; furthermore, we observed that a higher number of tumors had a strong linear effect on the risk of recurrence (Table 2 and Figure 1) and was also associated with a trend toward a higher probability for cancer mortality (Table 2), whereas CSS rates did not differ significantly among the 3 groups (Figure 2). In addition, the predictive impact of tumor numbers on recurrence was persistent in multivariate Cox regression analysis when all variables were included (Table 3). Thus, a higher number of tumors, particularly ≥3 tumor foci, is an independent predictor of disease recurrence and a possible indicator of cancer death in PTC. Extrathyroidal extension was another predictor for recurrence, consistent with previous studies [17–19]. However, some studies of the prognostic impact of tumor multifocality in papillary thyroid microcarcinoma (PTMC) have suggested that multifocal lesions do not appear to have prognostic impact in PTMC . Given the high incidence of multifocality in PTMC, 20-40% [21–23], further studies of small PTC tumors are needed to investigate the true role and influence of multifocality in this subtype of PTC.
At our center, TT was not routinely performed in PTC as an initial treatment. Considering the high incidence and negative influence of multifocality, extensive TT surgery is more likely to remove all disease foci and improve prognosis, particularly in PTC patients with ≥3 tumor foci and extrathyroidal extension. In addition, the rates of complications for permanent hypoparathyroidism and recurrent laryngeal nerve injury were both within the ranges of 0-4% and 0-6% reported in previous studies [24, 25], suggesting that thyroidectomy plus lymph node dissections can be performed safely and with low morbidity by experts.
Our study has several limitations that must be taken into account. First, since this study was a retrospective analysis, the prognostic significance of tumor foci has not been fully investigated. The long-term follow-up studies are needed to confirm the prognostic significance in PTC. Second, our study population was a cohort of patients cared for in a single center. Therefore, a much larger number of subjects in multicenter will be needed to generalize this results. At last, the fact that patients in China only receive radioiodine for distant metastases may significantly alter the outcome of patients in other countries where radioiodine is given, but this study certainly shows the impact of significant sized multifocality when radioiodine is not given.
We observed that a higher number of PTC foci was associated with a tendency toward more aggressive features, including greater primary tumor size, more frequent extrathyroidal extension and cervical LNM. In addition, an increase in the number of tumors was associated with an increased risk of recurrence and a trend toward more cancer mortality. Our findings suggested that the number of tumor foci could be used to assess the risk of poor prognosis, and TT is recommended in patients with more tumor foci.
Papillary thyroid cancer
Lymph node metastasis
Central lymph node metastasis
Lateral lymph node metastasis
Central lymph node dissection
Lateral lymph node dissection
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Address all correspondence and requests for reprints to Professor Qing-hai Ji, Department of Head and Neck Surgery, Fudan University Cancer Hospital, Department of Oncology, Shanghai Medical College, 270 Dong An Road, Shanghai 200032, People’s Republic of China. E-mail: firstname.lastname@example.org.
Source(s) of funding
This research is supported by grants from the National Natural Science Foundation of China (81272934) and the Natural Science Foundation of Shanghai (12ZR1406800).
- Siegel R, Ma J, Zou Z, Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 2014, 64 (1): 9-29. 10.3322/caac.21208.View ArticlePubMedGoogle Scholar
- Hung W, Sarlis NJ: Current controversies in the management of pediatric patients with well-differentiated nonmedullary thyroid cancer: a review. Thyroid. 2002, 12 (8): 683-702. 10.1089/105072502760258668.View ArticlePubMedGoogle Scholar
- Katoh R, Sasaki J, Kurihara H, Suzuki K, Iida Y, Kawaoi A: Multiple thyroid involvement (intraglandular metastasis) in papillary thyroid carcinoma. A clinicopathologic study of 105 consecutive patients. Cancer. 1992, 70 (6): 1585-1590. 10.1002/1097-0142(19920915)70:6<1585::AID-CNCR2820700623>3.0.CO;2-Z.View ArticlePubMedGoogle Scholar
- Jovanovic L, Delahunt B, McIver B, Eberhardt NL, Bhattacharya A, Lea R, Grebe SK: Distinct genetic changes characterise multifocality and diverse histological subtypes in papillary thyroid carcinoma. Pathology. 2010, 42 (6): 524-533. 10.3109/00313025.2010.508780.View ArticlePubMedGoogle Scholar
- Baek SK, Jung KY, Kang SM, Kwon SY, Woo JS, Cho SH, Chung EJ: Clinical risk factors associated with cervical lymph node recurrence in papillary thyroid carcinoma. Thyroid. 2010, 20 (2): 147-152. 10.1089/thy.2008.0243.View ArticlePubMedGoogle Scholar
- Leboulleux S, Rubino C, Baudin E, Caillou B, Hartl DM, Bidart JM, Travagli JP, Schlumberger M: Prognostic factors for persistent or recurrent disease of papillary thyroid carcinoma with neck lymph node metastases and/or tumor extension beyond the thyroid capsule at initial diagnosis. J Clin Endocrinol Metab. 2005, 90 (10): 5723-5729. 10.1210/jc.2005-0285.View ArticlePubMedGoogle Scholar
- Mazzaferri EL, Jhiang SM: Long-term impact of initial surgical and medical therapy on papillary and follicular thyroid cancer. Am J Med. 1994, 97 (5): 418-428. 10.1016/0002-9343(94)90321-2.View ArticlePubMedGoogle Scholar
- Chow SM, Law SC, Chan JK, Au SK, Yau S, Lau WH: Papillary microcarcinoma of the thyroid-Prognostic significance of lymph node metastasis and multifocality. Cancer. 2003, 98 (1): 31-40. 10.1002/cncr.11442.View ArticlePubMedGoogle Scholar
- Zhang L, Wei WJ, Ji QH, Zhu YX, Wang ZY, Wang Y, Huang CP, Shen Q, Li DS, Wu Y: Risk factors for neck nodal metastasis in papillary thyroid microcarcinoma: a study of 1066 patients. J Clin Endocrinol Metab. 2012, 97 (4): 1250-1257. 10.1210/jc.2011-1546.View ArticlePubMedGoogle Scholar
- Dinse GE, Lagakos SW: Nonparametric estimation of lifetime and disease onset distributions from incomplete observations. Biometrics. 1982, 38 (4): 921-932. 10.2307/2529872.View ArticlePubMedGoogle Scholar
- Gill RD: Multistate life-tables and regression models. Math Popul Stud. 1992, 3 (4): 259-276. 10.1080/08898489209525345.View ArticlePubMedGoogle Scholar
- Kim HJ, Park HK, Byun DW, Suh K, Yoo MH, Min YK, Kim SW, Chung JH: Number of tumor foci as predictor of lateral lymph node metastasis in papillary thyroid carcinoma. Head Neck. 2014, doi:10.1002/hed.23650Google Scholar
- Zhao Q, Ming J, Liu C, Shi L, Xu X, Nie X, Huang T: Multifocality and total tumor diameter predict central neck lymph node metastases in papillary thyroid microcarcinoma. Ann Surg Oncol. 2013, 20 (3): 746-752. 10.1245/s10434-012-2654-2.View ArticlePubMedGoogle Scholar
- Zhou YL, Gao EL, Zhang W, Yang H, Guo GL, Zhang XH, Wang OC: Factors predictive of papillary thyroid micro-carcinoma with bilateral involvement and central lymph node metastasis: a retrospective study. World J Surg Oncol. 2012, 10: 67-10.1186/1477-7819-10-67.View ArticlePubMedPubMed CentralGoogle Scholar
- Shepet K, Alhefdhi A, Lai N, Mazeh H, Sippel R, Chen H: Hereditary medullary thyroid cancer: age-appropriate thyroidectomy improves disease-free survival. Ann Surg Oncol. 2013, 20 (5): 1451-1455. 10.1245/s10434-012-2757-9.View ArticlePubMedGoogle Scholar
- Kim HJ, Sohn SY, Jang HW, Kim SW, Chung JH: Multifocality, but not bilaterality, is a predictor of disease recurrence/persistence of papillary thyroid carcinoma. World J Surg. 2013, 37 (2): 376-384. 10.1007/s00268-012-1835-2.View ArticlePubMedGoogle Scholar
- Edge SB, Compton CC: The American Joint Committee on Cancer: the 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 2010, 17 (6): 1471-1474. 10.1245/s10434-010-0985-4.View ArticlePubMedGoogle Scholar
- Ito Y, Tomoda C, Uruno T, Takamura Y, Miya A, Kobayashi K, Matsuzuka F, Kuma K, Miyauchi A: Clinical significance of metastasis to the central compartment from papillary microcarcinoma of the thyroid. World J Surg. 2006, 30 (1): 91-99. 10.1007/s00268-005-0113-y.View ArticlePubMedGoogle Scholar
- Wada N, Nakayama H, Suganuma N, Masudo Y, Rino Y, Masuda M, Imada T: Prognostic value of the sixth edition AJCC/UICC TNM classification for differentiated thyroid carcinoma with extrathyroid extension. J Clin Endocrinol Metab. 2007, 92 (1): 215-218. 10.1210/jc.2006-1443.View ArticlePubMedGoogle Scholar
- Riss JC, Peyrottes I, Chamorey E, Haudebourg J, Sudaka A, Benisvy D, Marcy PY, Nao EE, Demard F, Vallicioni J, Poissonnet G, Dassonville O, Santini J, Bozec A: Prognostic impact of tumour multifocality in thyroid papillary microcarcinoma based on a series of 160 cases. Eur Ann Otorhinolaryngol Head Neck Dis. 2012, 129 (4): 175-178. 10.1016/j.anorl.2011.11.003.View ArticlePubMedGoogle Scholar
- Ogilvie JB, Patel KN, Heller KS: Impact of the 2009 American Thyroid Association guidelines on the choice of operation for well-differentiated thyroid microcarcinomas. Surgery. 2010, 148 (6): 1222-1226. 10.1016/j.surg.2010.09.007. discussion 1226–1227View ArticlePubMedGoogle Scholar
- Mercante G, Frasoldati A, Pedroni C, Formisano D, Renna L, Piana S, Gardini G, Valcavi R, Barbieri V: Prognostic factors affecting neck lymph node recurrence and distant metastasis in papillary microcarcinoma of the thyroid: results of a study in 445 patients. Thyroid. 2009, 19 (7): 707-716. 10.1089/thy.2008.0270.View ArticlePubMedGoogle Scholar
- Connor MP, Wells D, Schmalbach CE: Variables predictive of bilateral occult papillary microcarcinoma following total thyroidectomy. Otolaryngol Head Neck Surg. 2011, 144 (2): 210-215. 10.1177/0194599810391616.View ArticlePubMedGoogle Scholar
- Moo TA, Umunna B, Kato M, Butriago D, Kundel A, Lee JA, Zarnegar R, Fahey TJ: Ipsilateral versus bilateral central neck lymph node dissection in papillary thyroid carcinoma. Ann Surg. 2009, 250 (3): 403-408.PubMedGoogle Scholar
- Koo BS, Choi EC, Yoon YH, Kim DH, Kim EH, Lim YC: Predictive factors for ipsilateral or contralateral central lymph node metastasis in unilateral papillary thyroid carcinoma. Ann Surg. 2009, 249 (5): 840-844. 10.1097/SLA.0b013e3181a40919.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/14/914/prepub
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