Table 1 Sequences for forward and reverse primers of the indicated target genes and the size expected for each PCR-amplified product
From: Altered glutamyl-aminopeptidase activity and expression in renal neoplasms
Enzyme | Gene symbol | Forward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|---|
APA | ENPEP | 5′-GCTCTCCTTGAACCACAAGACA-3′ | 5′-TTCTCTTCCCTTTTGAGATACTTGG-3′ | 133 |
Housekeeping Gene name | ||||
β-actin | ACTB | 5′-TCCCTGGAGAAGAGCTACGA-3′ | 5′-ATCTGCTGGAAGGTGGACAG-3′ | 362 |
Succinate dehydrogenase complex, subunit A | SDHA | 5′-TCTGCCCACACCAGCACT-3′ | 5′-CCTCTCCACGACATCCTTCC-3′ | 142 |
TATA box binding protein | TBP | 5′-GGATAAGAGAGCCACGAACCAC-3′ | 5′-TTAGCTGGAAAACCCAACTTCTG-3′ | 139 |
Peptidylpropyl isomerase A | PPIA | 5′-GGTCCCAAAGACAGCAGAAAA-3′ | 5′-TCACCACCCTGACACATAAACC-3′ | 114 |