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Table 1 Sequences for forward and reverse primers of the indicated target genes and the size expected for each PCR-amplified product

From: Altered glutamyl-aminopeptidase activity and expression in renal neoplasms

Enzyme Gene symbol Forward primer Reverse primer Amplicon size (bp)
APA ENPEP 5′-GCTCTCCTTGAACCACAAGACA-3′ 5′-TTCTCTTCCCTTTTGAGATACTTGG-3′ 133
Housekeeping Gene name     
β-actin ACTB 5′-TCCCTGGAGAAGAGCTACGA-3′ 5′-ATCTGCTGGAAGGTGGACAG-3′ 362
Succinate dehydrogenase complex, subunit A SDHA 5′-TCTGCCCACACCAGCACT-3′ 5′-CCTCTCCACGACATCCTTCC-3′ 142
TATA box binding protein TBP 5′-GGATAAGAGAGCCACGAACCAC-3′ 5′-TTAGCTGGAAAACCCAACTTCTG-3′ 139
Peptidylpropyl isomerase A PPIA 5′-GGTCCCAAAGACAGCAGAAAA-3′ 5′-TCACCACCCTGACACATAAACC-3′ 114
  1. Primers for the assayed housekeeping genes are also shown.