Figure 3

MYBBP1A induces anoikis in normal breast and breast cancer cells. (A) MYBBP1A induces anoikis in mammary epithelial cells. MCF-10A cells were transfected with siRNA for p53 or MYBBP1A; 48 h later, anoikis assay was performed. These cells were cultured under suspension conditions for 24 h, and viable cell numbers were determined by trypan blue dye exclusion or MTT assay. Protein levels of MYBBP1A and p53 in the cells were determined by immunoblotting. (B) and (C) MCF-7 and ZR-75-1 cells were transfected with siRNA for 48 h, followed by anoikis assay, or expressed a plasmid for p53 or MYBBP1A for 24 h followed by anoikis assay. These cells were cultured under suspension conditions for 24 h, and viable cell numbers were counted or assessed by MTT assay. Protein levels of MYBBP1A and p53 in these cells were determined by immunoblotting. Bars = mean + s.d. (n = 3).