MYBBP1A expression decreases as breast cancer carcinogenesis progresses. (A) Decreased expression of MYBBP1A mRNA in breast carcinomas from three independent datasets for human breast cancers. Data and statistics were obtained from the Oncomine database [20–22, 25]. (B) Decreased MYBBP1A protein expression in carcinoma samples of breast cancer patients. IHC was used to examine MYBBP1A expression in TMAs and compare it with that of human normal breast tissues and human breast cancer tissues. Counterstaining used hematoxylin. Representatives of normal breast tissue (1 and 2) and breast carcinoma tissue (3 to 6) are shown. Sample 3 is an infiltrating duct carcinoma of T2N1aM0, sample 4 is an infiltrating duct carcinoma of T3N2aM0, sample 5 is an infiltrating duct carcinoma of T2N0M0, and sample 6 is an infiltrating duct carcinoma of T2N0M0. The areas within the black squares are enlarged and shown in the right-side panels. Black scale bars = 0.5 mm and white scale bars = 100 μm. (C) MYBBP1A protein expression levels in MCF-10A, MCF-7, and ZR-75-1 cells. MYBBP1A protein levels in the cells were determined by immunoblottin.