Characterization of MCF7 cells stably depleted of flotillin-1 and flotillin-2. (A) Expression of flotillins in MCF7 cells depleted of flotillin-1 (sh-F1-A/B) or flotillin-2 (sh-F2-A/B). GAPDH was detected to show equal loading. (B) Densitometric quantification of flotillin-1 and flotillin-2. The signals were normalized to GAPDH. Bars represent the mean ± SD of three independent experiments. Statistical analysis using one-way ANOVA. ***, p < 0.001. (C) Staining of endogenous flotillin-1 and flotillin-2 in MCF7 cells depleted of flotillin-1 (sh-F1-A) or flotillin-2 (sh-F2-A). Scale bar: 20 μm.