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Table 1 Genes included on the angiogenesis PCR array and expressed by Caco-2 cells

From: Identification of the angiogenic gene signature induced by EGF and hypoxia in colorectal cancer

Symbol Gene name Description Stimulus
Hypoxia DMOG EGF EGF + DMOG
AKT1 PKB/PRKBA V-akt murine thymoma viral oncogene homolog 1 −1.1 1.3 1.2 1.1
ANGPT1 AGP1/AGPT Angiopoietin 1 2.3 2.1 1.6 2.3
ANGPTL3 ANGPT5 Angiopoietin-like 3 2.1 2.4 1.3 2.4
ANGPTL4 ANGPTL2/ARP4 Angiopoietin-like 4 3.1 3.9 1.4 5.8
ANPEP CD13/LAP1 Alanyl (membrane) aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150) 1.5 1.3 −1.4 1.4
CCL11 SCYA11 Chemokine (C-C motif) ligand 11 1.9 1.1 1.5 3.5
CCL2 GDCF-2/GDCF-2 HC11 Chemokine (C-C motif) ligand 2 1.4 −1.5 1.6 1.4
COL18A1 KNO Collagen, type XVIII, alpha 1 1.8 1.2 1.0 1.3
COL4A3 TUMSTATIN Collagen, type IV, alpha 3 (Goodpasture antigen) 1.7 −1.1 1.3 2.2
EDG1 CHEDG1/D1S3362 Endothelial differentiation, sphingolipid G-protein-coupled receptor 1, SIPR1 1.7 1.5 1.7 3.0
EFNA1 B61/ECKLG Ephrin-A1 2.6 2.4 1.0 2.0
EFNA3 EFL2/EPLG3 Ephrin-A3 7.2 6.6 1.3 4.9
EFNB2 EPLG5/HTKL Ephrin-B2 1.4 1.3 1.3 1.6
ENG CD105/END Endoglin 1.1 1.1 1.5 1.2
EPHB4 HTK/MYK1 EPH receptor B4 1.2 1.0 1.5 1.3
EREG ER Epiregulin 1.2 1.1 1.2 −1.9
FGFR3 ACH/CEK2 Fibroblast growth factor receptor 3 1.4 1.5 1.2 1.4
FLT1 FLT/VEGFR1 Fms-related tyrosine kinase 1 3.1 2.8 1.4 2.6
HIF1A HIF-1alpha Hypoxia-inducible factor 1, alpha −1.5 1.0 1.0 1.0
HPSE HPA/HPR1 Heparanase 1.7 1.9 1.1 1.8
ID1 ID Inhibitor of DNA binding 1, dominant negative helix-loop-helix protein −1.3 1.6 1.7 1.9
ID3 HEIR-1 Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein 1.5 −1.0 1.4 2.5
IGF1 IGFI Insulin-like growth factor 1 1.9 1.4 1.2 1.6
IL6 BSF2/HGF Interleukin 6 (interferon, beta 2) 1.9 1.3 1.5 1.7
IL-8 3-10C/AMCF-I Interleukin 8 1.9 −1.1 1.2 2.4
ITGAV CD51/MSK8 Integrin, alpha V (CD51) −1.1 1.4 1.9 1.9
ITGB3 CD61/GP3A Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) 1.3 −1.1 −1.2 2.4
JAG1 AGS/AHD Jagged 1 (Alagille syndrome) 1.1 1.6 1.6 2.7
KDR FLK1/VEGFR Kinase insert domain receptor 1.7 −1.2 −1.2 2.3
LAMA5 KIAA1907 Laminin, alpha 5 1.6 1.2 1.0 1.0
MMP2 CLG4/CLG4A Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) 1.7 1.5 −1.3 1.6
MMP9 CLG4B/GELB Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) 2.6 2.6 1.7 2.4
NOTCH4 INT3/NOTCH3 Notch homolog 4 (Drosophila) 1.5 −1.3 1.0 2.0
NRP1 DKFZp686A03134/DKFZp781F1414 Neuropilin 1 −1.6 −1.3 −1.5 −1.1
NRP2 NP2/NPN2 Neuropilin 2 −1.0 1.0 1.1 1.5
PDGFA PDGF-A/PDGF1 Platelet-derived growth factor alpha polypeptide 1.2 1.5 1.2 −1.2
PECAM1 CD31/PECAM-1 Platelet/endothelial cell adhesion molecule (CD31 antigen) 1.61 −1.3 1.0 1.7
PLAU ATF/UPA Plasminogen activator, urokinase 1.4 1.4 1.4 1.7
PLXDC1 TEM3/TEM7 Plexin domain containing 1 1.9 1.0 1.7 1.8
SERPINF1 EPC-1/PEDF Serpin peptidase inhibitor, clade F (pigment epithelium derived factor) 1.5 1.1 1.2 1.5
SPHK1 SPHK Sphingosine kinase 1 1.5 1.0 1.6 2.0
TGFA TFGA Transforming growth factor, alpha 1.9 1.4 1.5 2.3
TGFB1 CED/DPD1 Transforming growth factor, beta 1 5.4 4.3 1.4 4.6
TGFB2 TGF-beta2 Transforming growth factor, beta 2 1.7 1.0 1.0 1.8
TGFBR1 ACVRLK4/ALK-5 Transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53 kDa) 1.6 1.5 1.3 1.6
THBS1 THBS/TSP Thrombospondin 1 1.3 1.2 1.0 1.0
THBS2 TSP2 Thrombospondin 2 1.1 1.0 −1.2 −1.1
TIMP1 CLGI/EPA TIMP metallopeptidase inhibitor 1 1.3 −1.2 −1.1 1.1
TIMP2 CSC-21K TIMP metallopeptidase inhibitor 2 1.5 −1.2 −1.3 −1.1
TNFAIP2 B94 Tumour necrosis factor, alpha-induced protein 2 1.7 1.2 −1.1 1.4
VEGF VEGFA/VPF Vascular endothelial growth factor 3.1 3.4 1.0 3.1
  1. The Human Angiogenesis RT2 Profiler™ PCR Array was used to screen cDNA from Caco-2 cells exposed for 24 hours to either 1% O2, DMOG (1 mM), EGF (10 ng/ml) or a combination of EGF plus DMOG. Data were compared to HKG: ActB (β-actin), 18S rRNA, HPRT1 (hypoxanthine phosphoribosyltransferase 1) and RPL13A (60S ribosomal protein L13a), and are fold change versus untreated cells. Changes ≥2-fold are shown in bold. Data are from a representative array performed in duplicate using cDNA pooled from 3 different replicate experiments.