Figure 2

Immunofluorescence detection of Cx26, Cx32, Cx43, Cx46 (Alexa 546, red) and Ki67 (Alexa 488, green) in invasive ductal breast cancers prior to (pre-) and after (post-) neoadjuvant chemotherapy. Cell nuclei were stained using Hoescht (blue). Punctuate cell membrane associated and cytoplasmic Cx26 (1st row) and Cx32 (2^nd row) levels and the proliferating (green) tumour cell fractions were reduced after neoadjuvant therapy in cases originally scoring 3+. Cx43 (3^rd row) and Cx46 (4^th row) levels did not change after neoadjuvant chemotherapy in cases of high scores (3+). Pre-chemotherapy Cx43 and post-chemotherapy Cx46 samples are not stained for Ki67.