Table 1 Primer sequences
From: NOTCH1 mutations influence survival in chronic lymphocytic leukemia patients
Gene-exon-segment | Application | Forward primer | Reverse primer | Product size/bp | TA/°C |
|---|---|---|---|---|---|
Notch1- 26-a | PCR/SSCA | AGCCCCCTGTACGACCAGTA | CTTGCGCAGCTCCTCCTC | 283 | 63.5 |
Notch1- 26-b | PCR/SSCA | ACACGGCCAGCAGATGAT | GAGAGTTGCGGGGATTGAC | 231 | 57.1 |
Notch1- 27 | PCR/SSCA | GTGGCGTCATGGGCCTCA | TAGCAACTGGCACAAACAGC | 342 | 63.2 |
Notch1- 34a | PCR/SSCA | AACCACCTGCCTGGGATG | CGCATTGACCATTCAAACTG | 232 | 57.1 |
Notch1- 34b | PCR/SSCA | GGGCCCTGAATTTCACTGT | AGGCCCTGGTAGCTCATCAT | 229 | 60 |
Notch1- 34c | PCR/SSCA | GCTGCACAGTAGCCTTGCT | CTGAGCTCACGCCAAGGT | 224 | 58 |
Notch1- 34d | PCR/SSCA | ACATCCAGCAGCAGCAAAG | GTGGGACCAGCGAGGATG | 222 | 58 |
Notch1- 34e | PCR/SSCA | CACTATTCTGCCCCAGGAGA | CAGTCGGAGACGTTGGAATG | 234 | 58 |
Notch1- 34f | PCR/SSCA | ACAGCTACTCCTCGCCTGTG | AAGGCTTGGGAAAGGAAGC | 248 | 58 |
Notch1-6 | PCR/SSCA | GCAGCTGCCCGGGGCCGACA | TCAGGCCTGGCCCATGTGA | 330 | 62 |
Notch1-7 | PCR/SSCA | ATGCCTGGCCAGGGGCCGT | TCGACTTCTCATCGGTTCT | 273 | 58 |
Notch1-8 | PCR/SSCA | CCGATGGGGGTGGTGTGCAGT | TGCCCAGCCTCGACTCGGTT | 331 | 63 |
Notch1-11 | PCR/SSCA | AGTCCTAAGTCTTCCTGTGCC | AGGCCCGCCCTGCCCACT | 325 | 65 |
Notch1-12 | PCR/SSCA | AGGACTGACCGACACGTG | TCTGAGCACAGTGCAGTCA | 183 | 53 |
Notch1-13 | PCR/SSCA | TGGGCGCTGGGCCTCGGA | ACTGATGTGTCCCCATGA | 268 | 54 |
Gene-exon-segment | Application | Forward primer | Reverse primer | Product size/bp | T A /°C |
Notch2- 26-1 | PCR/SSCA | TTCTCTGCTTCCCCTTACCT | TTAATGCGCAGGTTGGTGT | 250 | 54.1 |
Notch2- 26-2 | PCR/SSCA | TGGTATTGATGCCACCTGAA | GCCTTGAAGTTCAGAAACCAA | 240 | 54.1 |
Notch2- 27 | PCR/SSCA | TACCCCCATCTCTCCTCCTC | AATTGTTCCCCCAATTGACA | 250 | 55.2 |
Notch2- 34-1 | PCR/SSCA | TCCCCTGTTGATTCCCTAGA | CACAATGTGGTGGTGGGATA | 249 | 55.2 |
Notch2- 34-2 | PCR/SSCA | GCACTGTGCTTCCCTCAGT | CTGCCTTTAGGGATGAGCTG | 298 | 55.2 |
Notch2- 34-3 | PCR/SSCA | ACCCATCCTGGCATAGCTC | TAGGCTGGGAGAATGGTCTG | 287 | 55.2 |
Notch2- 34-4 | PCR/SSCA | TTTGCCCAGTGTGGCTTT | GGTGATGAACTTGACCACTG | 249 | 57.1 |
Notch2- 34-5 | PCR/SSCA | ACACCCAGTCACAGTGGTCA | TGTCTCTACACTGGAGGTGGAC | 242 | 61.6 |