Figure 5

IL-11 fails to stimulate osteoclastogenesis in the absence of RANKL. (A) BMMs were cultured with rM-CSF (10 ng/ml) plus RANKL (100 ng/ml) as control or rM-CSF (10 ng/ml) plus IL-11 (5, 10 or 20 ng/ml) for 6 days in tissue culture dish. The cultures were then stained for TRAP activity. Each condition had three replicates (wells) and was repeated 4 times. A representative area of the culture from each condition is shown. (B) BMMS on bone slices were treated with rM-CSF (10 ;ng/ml) plus RANKL (100 ng/ml) as control or rM-CSF (10 ng/ml) plus IL-11 (20 ng/ml) for 9 days. Resorption pits were visualized by SEM. Magnification by SEM was 200x. Each resorption assay had two replicates (bone slices). Quantification of the bone resorption assays is shown, bars shown averaged ± S.E. *, p < 0.0001 (C) BMMs were cultured with rM-CSF (10 ng/ml) plus RANKL (100 ng/ml) as control or M-CSF (10 ng/ml) plus IL-11 (200 ng/ml) for 6 days in a tissue culture dish. The cultures were then stained for TRAP activity. Each condition had three replicates (wells) and was repeated 3 times. A representative area of the culture from each condition is shown.