Figure 4

IL-11 does not affect osteoclast survival. (A) BMMs were cultured with rM-CSF (10 ng/ml) plus RANKL (100 ng/ml) for 4 days until sufficient osteoclasts were formed and then cultured with rM-CSF (10 ng/ml) and RANKL (100 ng/ml) with vehicle (PBS) or IL-11 (10 ng/ml) for 8 days in tissue culture dish. The cultures were then stained for TRAP activity. Each condition had three replicates (wells) and was repeated 4 times. A representative area of the culture from each condition is shown. (B) Quantification of the osteoclastogenesis assays is shown in mean number of multinucleated TRAP-positive cells (>3 nuclei) per well. Bars show averages ± S.D.