MGMT, GATA6, CD81, DR4, and CASP8 gene promoter methylation in glioblastoma

BMC Cancer

Table 1 Primers for methylation-specific PCR

Gene	Forward primer 5´– 3´	Reverse primer 5´ – 3´	Tm
MGMT-M	TTTCGACGTTCGTAGGTTTTCGC	GCACTCTTCCGAAAACGAAACG	64
MGMT-U	TTTGTGTTTTGATGTTTGTAGGTTTTTGT	AACTCCACACTCTTCCAAAAACAAAACA	62
CD81-M	CGACGGCGGCGATTTTATCGC	GACCTACGAAACGCGAACCG	58
CD81-U	GTGATGGTGGTGATTTTATTGT	ACAACCTACAAAACACAAACCAA	58
GATA6-M	CGGGGTAGATTTCGGATTCGC	CAACCGAACCTCGAACGAACG	60
GATA6-U	GTGTGGGGTAGATTTTGGATTTGT	AAACAACCAAACCTCAAACAAACA	60
DR4-M	TTCGAATTTCGGGAGCGTAGC	GTAATTCAATCCTCCCCGCGA	60
DR4-U	GTAGTGATTTTGAATTTTGGGAGTGTAGT	CTCATAATTCAATCCCCACAA	60
CASP8-M	TAGGGGATTCGGAGATTGCGA	CGTATATCTACATTCGAAACGA	58
CASP8-U	TAGGGGATTTGGAGATTGTGA	CCATATATATCTACATTCAAAACAA	58

ISSN: 1471-2407