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Table 2 Apoptosis measured by Annexin V-Alexa Fluor 488/PI staining

From: A human ribonuclease induces apoptosis associated with p21WAF1/CIP1induction and JNK inactivation

  Control PE5 Onconase
  24 h 48 h 72 h 24 h 48 h 72 h 24 h 48 h 72 h
Early apoptotic cells (%) 4.1 ± 0.2 4.2 ± 0.5 4.5 ± 0.7 3.9 ± 0.1 17.7 ± 1.9 28.3 ± 1.3 6.8 ± 2.1 11.9 ± 1.1 20.4 ± 2.0
Late apoptotic cells (%) 5.9 ± 0.2 7.2 ± 0.9 5.7 ± 0.1 0.7 ± 0.3 6.5 ± 1.4 26.0 ± 4.4 2.3 ± 0.1 11.6 ± 2.6 25.9 ± 1.4
Necrotic cells (%) 2.4 ± 0.3 2.4 ± 0.7 1.6 ± 0.2 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0
Viable cells (%) 87.6 ± 0.6 86.2 ± 1.1 88.2 ± 1.0 95.4 ± 0.4 75.8 ± 0.5 45.7 ± 5.3 90.9 ± 2.0 76.5 ± 3.7 53.7 ± 0.6
  1. NCI/ADR-RES cells were treated with 35 μM PE5 or 5 μM onconase for 24, 48 and 72 h, stained with Annexin V-Alexa 488 and PI and analyzed by flow cytometry. Cells undergoing early apoptosis were positive for Annexin V and negative for PI (Annexin V+/PI-), late apoptotic cells were Annexin V+/PI+ and necrotic cells were Annexin V-/PI+. All data were expressed as mean ± SE of three different experiments. Values were analyzed from 10,000 total events.