Table 2 Apoptosis measured by Annexin V-Alexa Fluor 488/PI staining
From: A human ribonuclease induces apoptosis associated with p21WAF1/CIP1induction and JNK inactivation
Control | PE5 | Onconase | |||||||
|---|---|---|---|---|---|---|---|---|---|
24 h | 48 h | 72 h | 24 h | 48 h | 72 h | 24 h | 48 h | 72 h | |
Early apoptotic cells (%) | 4.1 ± 0.2 | 4.2 ± 0.5 | 4.5 ± 0.7 | 3.9 ± 0.1 | 17.7 ± 1.9 | 28.3 ± 1.3 | 6.8 ± 2.1 | 11.9 ± 1.1 | 20.4 ± 2.0 |
Late apoptotic cells (%) | 5.9 ± 0.2 | 7.2 ± 0.9 | 5.7 ± 0.1 | 0.7 ± 0.3 | 6.5 ± 1.4 | 26.0 ± 4.4 | 2.3 ± 0.1 | 11.6 ± 2.6 | 25.9 ± 1.4 |
Necrotic cells (%) | 2.4 ± 0.3 | 2.4 ± 0.7 | 1.6 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
Viable cells (%) | 87.6 ± 0.6 | 86.2 ± 1.1 | 88.2 ± 1.0 | 95.4 ± 0.4 | 75.8 ± 0.5 | 45.7 ± 5.3 | 90.9 ± 2.0 | 76.5 ± 3.7 | 53.7 ± 0.6 |