Expression of cyclin D1, cyclin E, p21
WAF1/CIP1 and p27
KIP1 in 7 μM PE5-treated cells. 72 h after the treatments, cells were harvested and analyzed by Western blot using the antibodies described in the Methods section. A) Western blot of a representative experiment. B) Densitometric analysis of the GAPDH-normalized immunoblots of each treatment respective to that of untreated cells. White bars indicate untreated cells (control) and black bars indicate PE5-treated cells.