- Research article
- Open Access
- Open Peer Review
Single-agent pegylated liposomal doxorubicin (PLD) in the treatment of metastatic breast cancer: results of an Austrian observational trial
© Fiegl et al; licensee BioMed Central Ltd. 2011
- Received: 10 January 2011
- Accepted: 24 August 2011
- Published: 24 August 2011
In advanced breast cancer, multiple sequential lines of treatments are frequently applied. Pegylated liposomal doxorubicin (PLD) has a favourable toxicity profile and can be used in first or higher lines of therapy. PLD has demonstrated response activity even after prior anthracycline exposure.
129 consecutive patients with advanced breast cancer, of whom the majority had been massively pretreated, received PLD as monotherapy within licensed approval, for which efficacy and toxicities were documented.
In a routine therapy setting, PLD was administered in a slightly reduced dose (median, 40 mg/m2 per cycle). Response rate (complete and partial remission) was 26%, and stable disease was observed in 19% of patients. Progression-free (PFS) and overall survival (OS) were 5.8 months and 14.2 months, respectively. There was no difference in terms of response and PFS, no matter if patients had already received anthracycline treatment. Interestingly, PFS proved similar regardless whether PLD was administered as palliative therapy in first, second or third line. Furthermore, PFS and OS were similar in patients with response or stable disease, underscoring the view that disease stabilization is associated with a profound clinical benefit. The most common side effects reported were palmar-plantar erythrodysesthesia (17%), exanthema (14%) and mucositis (12%).
Efficacy and toxicity data in these "real life" patients permit the conclusion that PLD is a valuable option in the treatment of advanced breast cancer even in heavily pretreated patients.
- Overall Survival
- Metastatic Breast Cancer
- Advanced Breast Cancer
- Pegylated Liposomal Doxorubicin
- Cardiac Risk Factor
There are numerous systemic therapeutic options available for the tailored treatment of metastatic breast cancer. In addition, therapeutic antibodies and small molecules increasingly complement the therapeutic range of instruments. The choice of systemic therapy follows accepted guidelines (1) but remains largely based on individual factors (2). In case instant response is required, such as rapidly progressive, threatening disease or massive symptoms, combination cytostatic therapy is usually administered; more often, however, monotherapy is the method of choice for patients in whom a long-term stabilization of metastatic disease is the objective. Thus, different therapies can be applied in sequence over a long period of metastatic disease. Although evidence from randomized studies is largely lacking in more advanced disease, administration of multiple treatment lines (e.g., third line and higher) is clinical routine, often drawing out remarkable clinical benefit. However, side effects and cumulative toxicities have to be considered in the choice of a cytostatic to be administered.
Pegylated liposomal doxorubicin (PLD; Caelyx, Aesca, Vienna) is doxorubicin encapsulated in 85 nm vesicles with a lipophilic surface including hydrophilic polyethylene glycol. With this doxorubicin formulation, a prolonged circulation time and differential uptake in abnormally permeable vessels with accumulation in tumor tissue is observed (3). The main advantage of PLD, apart from the clinical benefit provided for breast cancer, is a reduced proportion of patients with classic anthracycline side effects such as cardiotoxicity, hematotoxicity and alopecia (4-10); on the other hand, PLD has been associated with stomatitis and palmar-plantar erythrodysesthesia (PPE), which may prove to be dose-limiting after repeated treatments (10-12).
In an Austrian phase II trial on PLD as second-line monotherapy in metastatic breast cancer, encouraging results with a high rate of clinical benefit at good tolerability were achieved (4). Based on these encouraging results, an observational study was undertaken in Austria to document benefit and toxicities of PLD monotherapy in patients with metastatic breast cancer and cardiac risk factors, who were treated in a routine use setting.
Inclusion criteria and treatment
In accordance with licensed approval and accepted guidelines for the treatment of advanced breast cancer (1), PLD was administered as monotherapy in patients with increased cardiac risk. In this survey on PLD use in Austria, it was prespecified that patients had histologically confirmed, metastatic breast cancer with indication for cytostatic therapy, were older than age 18, and harbored at least one of the following features which might compromise cardiac function: age > 60 years; prior thoracic irradiation (including adjuvant irradiation of the breast); prior anthracycline based therapy; hypertension; and history of cardiac disease, such as infarction and congestive heart failure. Echocardiography results were not documented in this observational study. According to the manufacturer's recommendation, PLD was scheduled in a dose of 50 mg/m2 once every 28 days for 6 cycles. In this survey, aiming at analysing data on PLD use in breast cancer treatment in a "real life" setting, PLD doses and their modification were documented cycle by cycle. The last follow-up data available for this analysis was obtained on 29 September 2010, and the median observation time in this study was 10.1 months.
This was an observational study with treatment of breast cancer by PLD within licensed approval; thus, adhering to the guidelines of the ethics committee of Innsbruck Medical University, formal ethical approval was not required at the time the trial was initiated. Patients were routinely informed about the nature of therapy, but written informed consent did not need to be obtained.
Patients who received at least one dose of PLD were assessable for efficacy and safety. Response evaluation was performed after two to four cycles ("mid term" staging) and after completion of therapy, i.e. after 6 cycles as scheduled in the protocol or prior termination in case of progressive disease (PD) or intolerable toxicity. Thus, definitive response was determined at the end of PLD therapy. However, to include temporary therapeutic benefit, an alternative response evaluation was performed, yielding "best response" which was documented separately at both points in time. Response was evaluated according to RECIST criteria for target and non-target lesions, but no central review of radiogramm was conducted. Progression-free survival (PFS) was defined as time from first dose of PLD to disease progression or death, whichever occurred first. Overall survival (OS) was measured from initiation of PLD therapy until final follow-up or death from any cause.
Comparison of response rates between patient subgroups at baseline was carried out using Pearson's chi-square test. Event-related data (PFS and OS) were estimated using the Kaplan-Meier method, and comparison of outcomes between categorical subgroups was made using the log-rank test. Univariate analysis was used to determine which baseline characteristics were associated with PFS and OS. For those found to be associated with PFS and OS (P ≤0.10), hazard ratios were calculated using the multivariate Cox regression analysis. The significance level was set at P < 0.05, in two-sided tests.
Patient demographics and baseline charactersitics (n = 129)
Median age (range), years
63 (34 - 86)
Median time from diagnosis to PLD (range), years
5.9 (0.1 - 26.7)
Distribution of metastasis
Skin and sof tissue
Metastatic sites involved
Four or more sites
Cardiac risk factors
Prior anthracycline therapy
Age > 60 years
Prior thoracic irradiation†
History of cardiac disease
Median number of cardiac risk factors (range)
2 (1 - 5)
Previous therapies, setting
Endocrine (adjuvant and/or metastatic)
Chemotherapy (adjuvant and/or metastatic)
Anthracycline containing (adjuvant and/or metastatic)
Taxane containing (adjuvant and/or metastatic)
PLD line of chemotherapy‡
Fourth and higher
Median number of PLD cycles (range)§
Median cumulative PLD dose, mg/m2 (range) §
210 (25 - 300)
Response to PLD by baseline characteristics
Response according RECIST after completion of PLD
No. of patients (%)
> = 63
No. cardiac risk factors
1 - 2
No. metastatic sites
No. of prior chemos*
0 - 3
In line with a previous investigation (6), ORR upon PLD was independent of prior anthracycline exposure. However, ORR was significantly decreased in patients who had previously received a taxane or both anthracycline and taxane (Table 2).
In the 17 patients with extended PLD therapy (i.e., > 6 cycles), prolonged disease stabilization until termination of PLD was observed in 10 patients, whereas the remaining patients experienced disease progression while on PLD.
Univariate analysis for association of baseline characteristics and therapeutic response with PFS and OS from start of PLD.
Number of patients
Median PFS, months
Median OS, months
No. cardiac risk factors
1 - 2
No. metastatic sites
Prior endocrine therapy
No. of prior chemos+
0 - 3
6 cycles, no extension
> 6 cycles
Multivariate Cox regression analysis was performed, and pretreatment baseline characteristics that showed association with PFS in the univariate analysis (Table 3) were included. Independent risk factors for shorter PFS were: greater number (≥4) of metastatic sites (hazard ratio, 1.66; 95% confidence interval [95%CI], 1.05-2.61; P = 0.029); prior taxane-containing therapy (hazard ratio, 1.52; 95%CI, 1.01-2.29; P = 0.044); and greater number (≥4) of prior lines of therapy (hazard ratio, 1.79; 95%CI, 1.15-2.77; P = 0.009).
At the last follow up, 90 patients had died. Median OS after inititiation of PLD was 14.2 months. OS was significantly shorter in patients with ≥4 metastatic sites and ≥4 prior therapy lines (Table 3). In contrast to PFS, there was a trend towards shorter OS in patients who had had prior anthracycline exposure. In analogy with PFS, patients experiencing disease stabilization had similar OS to those with CR/PR. There was no difference in OS in patients having received > 6 cycles when compared with patients with clinical benefit who discontinued PLD after 6 cycles (Table 3).
In multivariate Cox regression analysis (including the characteristics "number of metastatic sites"; "number of prior chemotherapies"; "prior anthracycline"; and "prior endocrine therapy"), the occurrence of a greater number (≥4) of metastatic sites was the only independent risk factor for shorter OS (hazard ratio, 2.78; 95%CI, 1.75-4.42; P < 0.001).
Therapies after PLD
Following disease progression after PLD, 79 patients received another line of palliative systemic therapy, whereas 36 patients did not receive further therapy; in the remaining 14 patients, status of subsequent therapy is not known. The most common subsequent therapies were: endocrine therapy (n = 20); single agent chemotherapy (gemcitabine [n = 11]; taxane ; capecitabine ; platinum derivates , PLD reinduction ; vinorelbine ; non-pegylated liposomal doxorubicin ; ralitrexed ), combination chemotherapy (CMF ); chemotherapy in combination with monoclonal antibodies (docetaxel/trastuzumab ; capecitabine/trastuzumab ; capecitabine/bevacizumab ) or another biologic compound (capecitabine/lapatinib ; capacitabine/bortezomib ; and monotherapy with a tyrosine kinase inhibitor (lapatinib ; gefitinib ).
Toxic effects occurring during treatment of advanced breast cancer with PLD (described in at least 2 PLD-treated patients).
Alopecia (only grade 1 and 2)
Neutropenia grade 4
Thrombocytopenia grade 4
In this observational phase IV study, encouraging results with single-agent PLD in metastasized breast cancer were observed. It is a well known fact that a considerable portion of patients with cancer would not be eligible for inclusion in prospective studies (13), e.g. due to numerous comorbidities and a large number of prior therapies; moreover, data on efficacy of antineoplastic drugs in unselected patients, often compromised by unfavorable performance status, are rare. This study is part of the authors' ongoing efforts to study effects of various treatments in a range of malignancies in a "real life" setting (14-18). Thus, we aimed at defining the role of PLD in 129 consecutive breast cancer patients who were treated within the licensed approval. It must be noted that in an observational study such as ours, patients differ from those in prospective studies. This is especially true with respect to basic characteristics (in the study presented here, there were no exclusion criteria) and in-study procedures. For example, the interval of regular restaging was not strictly pre-specified, a fact which may influence PFS to a certain extent.
We were able to demonstrate that therapeutic results and toxicity rates observed in prospective studies translate nicely into the routine setting (4-11). In the course of data documentation, it was verified that the participating institutions started PLD therapy along the proposed guidelines of administration and had their patients under tight clinical control. Generally, however, we realized that individualization of PLD schedule, predominantly through dose reductions, was frequently carried out, well in line with the observation by other groups (5, 6, 10, 19, 20). In fact, the median dose of PLD administered was 40 mg/m2 per cycle. This dose is less toxic than the licensed 50 mg/m2 per cycle, obviously without compromising therapeutic efficacy (20). Thus, we are convinced that the results presented in our study reflect what is achievable in breast cancer treatment in the routine setting. The data presented here could serve as a benchmark for other surveys on the use of cytostatic monochemotherapy in breast cancer.
We found comparatively low objective response rates and a comparatively high proportion of patients with minor response and disease stabilization, qualifying for SD. Age, prior endocrine or anthracycline therapy did not affect response probability, and response rates were identical between chemo-naive patients and those with one to three prior chemotherapy lines. However, response rate was significantly lower in patients who had had four or more prior therapy lines, a cohort which was relatively large in this survey (26%, Table 2). We observed a higher ORR in the patients who could receive the full course of scheduled six PLD cycles (31/67 patients [49%]), and it became evident that response to PLD can manifest relatively late during therapy. Thus, 9 of the 31 responding patients (29%) had SD in the mid-term staging after two to four cycles.
It appeared that PLD therapy offered favorable survival benefit in the different lines of treatment, comparable up to the third line of palliative therapy, a fact that has not been described yet (Figure 1). This suggests that PLD is a reasonable treatment option even in higher treatment lines when other cytostatic drugs are no longer promising. However, by multivariate analysis, escalating refractoriness also towards PLD, if administered in fourth and higher line, is highlighted; likewise, inferior clinical efficacy by PLD is observed in extraordinarily advanced disease, with 4 or more different metastatic sites involved. Nevertheless, we observed impressive disease stabilizations in a number of patients harboring such unfavourable characteristics, and future work should focus on defining features predicting therapeutic efficacy in such patients.
Patients experienced profound benefit in terms of PFS and OS if they responded to or, more interestingly, had disease stabilization through PLD therapy (Figure 2). This underscores the observation that the achievment of SD by systemic therapy is frequently associated with profound clinical benefit not solely by symptom improvement and gain in quality of life, but also by prolongation of survival (8, 14, 21, 22). In this observational trial, we did not find any indication that extending therapy beyond 6 cycles could be beneficial in terms of PFS or OS. In the GEICAM 2001-01 study, PLD was given as switch maintenance therapy after first line induction by doxorubicin followed by docetaxel. A modest prolongation of time to next therapy but not of OS was observed (23); a general recommendation for PLD use as maintenance therapy cannot be given (24).
In this study, frequencies of toxicities induced by PLD are within the range of expectations (4, 5, 10-12, 19). The typical side effects of PLD therapy, PPE and exanthema (Table 4), appeared to be of minor relevance, probably due to the intensified managment of these toxicities according to the manufacturer's recommendations and published guidelines (25). The lower median dose of PLD may add to this observation (20). The patients in this observational study harbored, to varying extents, cardiac risk. The percentage of patients with cardiac toxicities was small; however, two severe cardiac events (left ventricular dysfunction) occurred during PLD treatment phase, one of which was fatal. Thus, it is emphasized that close monitoring of patients with cardiac risk factors is essential during cytostatic chemotherapy, by frequent clinical examination, and repeated echocardiography and laboratory testing (e.g. NT-proBNP)(26). Grade IV neutropenia and thrombocytopenia occurred in three patients and two patients, respectively; two of them were massively affected by bone marrow carcinosis and, thus, had impaired hematopoietic reserve. Remarkably, partial regeneration of hematopoiesis was achieved after 3 cycles of dose-reduced PLD applications in one of the two patients with grade IV thrombocytopenia.
The results of this observational study in metastatic breast cancer document well the role of PLD in clinical practice. PLD is a valuable single-agent treatment option in advanced breast cancer, and particularly, for patients with cardiac risk factors and those who had prior therapies including anthracycline.
Other contributors to this study: G. Kopetzki (St. Pölten), J. Schüller, P. Sevelda, C. Wogritsch (Vienna), A. Leitner (Hallein), W. Halbritter (Bad Vöslau), R. Buketits (Baden), E. Petru (Graz), H. Rabl, F. Peintinger (Leoben), H. Seewann (Fürstenfeld), G. Engelich (Wiener Neustadt), K. Schwammberger (Salzburg), K. Unterrieder (Villach). The authors thank Jeffrey McCabe for editorial support. This study was supported by the Association for Tumor Research (Hemato-Oncology, Uni-Clinic Innsbruck) and by a grant from Aesca Pharma Austria.
- Beslija S, Bonneterre J, Burstein HJ, Cocquyt V, Gnant M, Heinemann V, Jassem J, Köstler WJ, Krainer M, Menard S, Petit T, Petruzelka L, Possinger K, Schmid P, Stadtmauer E, Stockler M, Van Belle S, Vogel C, Wilcken N, Wiltschke C, Zielinski CC, Zwierzina H, Central European Cooperative Oncology Group (CECOG): Third consensus on medical treatment of metastatic breast cancer. Ann Oncol. 2009, 20: 1771-85. 10.1093/annonc/mdp261.View ArticlePubMedGoogle Scholar
- Prassl K, Sahanic A, Reicher B, Denz H, Freund M, Fiegl M: Striking response with bevacizumab and chemotherapy in a woman with heavily pretreated breast cancer: a case presentation. Memo. 2008, 1: 149-51.View ArticleGoogle Scholar
- Northfelt DW, Martin FJ, Working P, Volberding PA, Russell J, Newman M, Amantea MA, Kaplan LD: Doxorubicin encapsulated in liposomes containing surface-bound polyethylene glycol: pharmacokinetics, tumor localization, and safety in patients with AIDS-related Kaposi's sarcoma. J Clin Pharmacol. 1996, 36: 55-63.View ArticlePubMedGoogle Scholar
- Mlineritsch B, Mayer P, Rass C, Reiter E, Russ G, Vesenmayer G, Oberaigner W, Hausmaninger H: Phase II study of single-agent pegylated liposomal doxorubicin HCl (PLD) in metastatic breast cancer after first-line treatment failure. Onkologie. 2004, 27: 441-46. 10.1159/000080363.PubMedGoogle Scholar
- Huober J, Fett W, Nusch A, Neise M, Schmidt M, Wischnik A, Gerhardt S, Goehler T, Lück HJ, Rost A: A multicentric observational trial of pegylated liposomal doxorubicin for metastatic breast cancer. BMC Cancer. 2010, 10: 2-10.1186/1471-2407-10-2.View ArticlePubMedPubMed CentralGoogle Scholar
- Al-Batran SE, Bischoff J, von Minckwitz G, Atmaca A, Kleeberg U, Meuthen I, Morack G, Lerbs W, Hecker D, Sehouli J, Knuth A, Jager E: The clinical benefit of pegylated liposomal doxorubicin in patients with metastatic breast cancer previously treated with conventional anthracyclines: a multicentre phase II trial. Br J Cancer. 2006, 94: 1615-20.PubMedPubMed CentralGoogle Scholar
- Ranson MR, Carmichael J, O'Byrne K, Stewart S, Smith D, Howell A: Treatment of advanced breast cancer with sterically stabilized liposomal doxorubicin: results of a multicenter phase II trial. J Clin Oncol. 1997, 15: 3185-91.PubMedGoogle Scholar
- Lyass O, Uziely B, Ben-Yosef R, Tzemach D, Heshing NI, Lotem M, Brufman G, Gabizon A: Correlation of toxicity with pharmacokinetics of pegylated liposomal doxorubicin (Doxil) in metastatic breast carcinoma. Cancer. 2000, 89: 1037-47. 10.1002/1097-0142(20000901)89:5<1037::AID-CNCR13>3.0.CO;2-Z.View ArticlePubMedGoogle Scholar
- O'Brien ME, Wigler N, Inbar M, Rosso R, Grischke E, Santoro A, Catane R, Kieback DG, Tomczak P, Ackland SP, Orlandi F, Mellars L, Alland L, Tendler C: CAELYX Breast Cancer Study Group. Reduced cardiotoxicity and comparable efficacy in a phase III trial of pegylated liposomal doxorubicin HCl (CAELYX/Doxil) versus conventional doxorubicin for first-line treatment of metastatic breast cancer. Ann Oncol. 2004, 15: 440-49. 10.1093/annonc/mdh097.View ArticlePubMedGoogle Scholar
- Keller AM, Mennel RG, Georgoulias VA, Nabholtz JM, Erazo A, Lluch A, Vogel CL, Kaufmann M, von Minckwitz G, Henderson IC, Mellars L, Alland L, Tendler C: Randomized phase III trial of pegylated liposomal doxorubicin versus vinorelbine or mitomycin C plus vinblastine in women with taxane-refractory advanced breast cancer. J Clin Oncol. 2004, 22: 3893-901. 10.1200/JCO.2004.08.157.View ArticlePubMedGoogle Scholar
- Coleman RE, Biganzoli L, Canney P, Dirix L, Mauriac L, Chollet P, Batter V, Ngalula-Kabanga E, Dittrich C, Piccart M: A randomised phase II study of two different schedules of pegylated liposomal doxorubicin in metastatic breast cancer (EORTC-10993). Eur J Cancer. 2006, 42: 882-87. 10.1016/j.ejca.2005.12.011.View ArticlePubMedGoogle Scholar
- Lorusso D, Di Stefano A, Carone V, Fagotti A, Pisconti S, Scambia G: Pegylated liposomal doxorubicin-related palmar-plantar erythrodysesthesia ('hand-foot' syndrome). Ann Oncol. 2007, 18: 1159-64. 10.1093/annonc/mdl477.View ArticlePubMedGoogle Scholar
- Vardy J, Dadasovich R, Beale P, Boyer M, Clarke SJ: Eligibility of patients with advanced non-small cell lung cancer for phase III chemotherapy trials. BMC Cancer. 2009, 9: 130-10.1186/1471-2407-9-130.View ArticlePubMedPubMed CentralGoogle Scholar
- Fiegl M, Falkner A, Hopfinger G, Brugger S, Zabernigg A, Bauer F, Haslbauer F, Demirtas D, Grossschmidt P, Tatzreiter G, Gastl G, Greil R, Austrian Collaborative Study Group on Alemtuzumab in Chronic Lymphocytic Leukemia: Routine clinical use of alemtuzumab in patients with heavily pretreated B-cell chronic lymphocytic leukemia: a nation-wide retrospective study in Austria. Cancer. 2006, 107: 2408-16. 10.1002/cncr.22263.View ArticlePubMedGoogle Scholar
- Bartsch R, Wenzel C, Altorjai G, Pluschnig U, Rudas M, Mader RM, Gnant M, Zielinski CC, Steger GG: Capecitabine and trastuzumab in heavily pretreated metastatic breast cancer. J Clin Oncol. 2007, 25: 3853-8. 10.1200/JCO.2007.11.9776.View ArticlePubMedGoogle Scholar
- Bartsch R, De Vries C, Pluschnig U, Dubsky P, Bago-Horvath Z, Gampenrieder SP, Rudas M, Mader RM, Rottenfusser A, Wiltschke C, Gnant M, Zielinski CC, Steger GG: Predicting for activity of second-line trastuzumab-based therapy in her2-positive advanced breast cancer. BMC Cancer. 2009, 9: 367-10.1186/1471-2407-9-367.View ArticlePubMedPubMed CentralGoogle Scholar
- Bartsch R, Mlineritsch B, Gnant M, Niernberger T, Pluschnig U, Greil R, Wenzel C, Sevelda P, Thaler J, Rudas M, Pober M, Zielinski CC, Steger GG, Austrian Fulvestrant Registry: The Austrian fulvestrant registry: results from a prospective observation of fulvestrant in postmenopausal patients with metastatic breast cancer. Breast Cancer Res Treat. 2009, 115: 373-80. 10.1007/s10549-008-0132-0.View ArticlePubMedGoogle Scholar
- Fiegl M, Hilbe W, Auberger J, Schmid T, Auberger T, Tzankov A, Sterlacci W, Denz H, Jamnig H, Greil R: Twelve-year retrospective analysis of lung cancer--The TYROL Study: Daily routine in 1,424 patients (1995-2006). J Clin Oncol. 2008, 26 (15S): 19063-Google Scholar
- Perez AT, Domenech GH, Frankel C, Vogel CL: Pegylated liposomal doxorubicin (Doxil) for metastatic breast cancer: the Cancer Research Network, Inc., experience. Cancer Invest. 2002, 20 (Suppl 2): 2-29.Google Scholar
- Al-Batran SE, Meerpohl HG, von Minckwitz G, Atmaca A, Kleeberg U, Harbeck N, Lerbs W, Hecker D, Sehouli J, Knuth A, Jager E: Reduced incidence of severe palmar-plantar erythrodysesthesia and mucositis in a prospective multicenter phase II trial with pegylated liposomal doxorubicin at 40 mg/m2 every 4 weeks in previously treated patients with metastatic breast cancer. Oncology. 2006, 70: 141-46. 10.1159/000093005.View ArticlePubMedGoogle Scholar
- Sun WL, Hutarew G, Gradl J, Gratzl M, Heumader E, Denz H, Fiegl M: Bevacizumab-based combination therapy in a patient with pseudomyxoma peritonei. Memo. 2009, 2: 113-15. 10.1007/s12254-009-0113-y.View ArticleGoogle Scholar
- Al-Batran SE, Güntner M, Pauligk C, Scholz M, Chen R, Beiss B, Stopatschinskaja S, Lerbs W, Harbeck N, Jäger E: Anthracycline rechallenge using pegylated liposomal doxorubicin in patients with metastatic breast cancer: a pooled analysis using individual data from four prospective trials. Br J Cancer. 2010, 103: 1518-23. 10.1038/sj.bjc.6605961.View ArticlePubMedPubMed CentralGoogle Scholar
- Alba E, Ruiz-Borrego M, Margelí M, Rodríguez-Lescure A, Sánchez-Rovira P, Ruiz A, Mel-Lorenzo JR, Ramos-Vázquez M, Ribelles N, Calvo E, Casado A, Márquez A, Vicente D, García-Sáenz JA, Martín M: Maintenance treatment with pegylated liposomal doxorubicin versus observation following induction chemotherapy for metastatic breast cancer: GEICAM 2001-01 study. Breast Cancer Res Treat. 2010, 122: 169-76. 10.1007/s10549-010-0860-9.View ArticlePubMedGoogle Scholar
- Murphy CG, Khasraw M, Seidman AD: Holding back the sea: the role for maintenance chemotherapy in metastatic breast cancer. Breast Cancer Res Treat. 2010, 122: 177-9. 10.1007/s10549-010-0925-9.View ArticlePubMedGoogle Scholar
- Von Moos R, Thuerlimann BJ, Aapro M, Rayson D, Harrold K, Sehouli J, Scotte F, Lorusso D, Dummer R, Lacouture ME, Lademann J, Hauschild A: Pegylated liposomal doxorubicin-associated hand-foot syndrome: recommendations of an international panel of experts. Eur J Cancer. 2008, 44: 781-90. 10.1016/j.ejca.2008.01.028.View ArticlePubMedGoogle Scholar
- Broeyer FJ, Osanto S, Ritsema van Eck HJ, van Steijn AQ, Ballieux BE, Schoemaker RC, Cohen AF, Burggraaf J: Evaluation of biomarkers for cardiotoxicity of anthracyclin-based chemotherapy. J Cancer Res Clin Oncol. 2008, 134: 961-68. 10.1007/s00432-008-0372-8.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/11/373/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.