Table 1 Primer pairs used for RT-PCR.
From: Oncostatin M promotes STAT3 activation, VEGF production, and invasion in osteosarcoma cell lines
Gene | Primers (forward/reverse) | Annealing Temp (°C) |
|---|---|---|
Canine IL-6 | (5'-gagattccaaggatgatg/gcctctttgctgtcttcaca-3') | 55 |
Canine IL-6R | (5'-gagatctgtgcagctcagcgactc/ctccactcacagaatacc-3') | 55 |
Canine OSM | (5'-gtccttggactcctgttcctg/cactcagcatctccaagtc-3') | 55 |
Canine OSMR | (5'-gtaaggtggaccgaggagatg/gagcaagccagtgtctctc-3') | 55 |
Canine gp130 | (5'-gaagcccagtccacc/ccttcatacaacgaatcc-3') | 55 |
Canine GAPDH | (5'-accacagtccatgccatcac/tccaccaccctgttgctgta-3') | 55 |
Human IL-6 | (5'-gctgctcctggtgttgcctg/ggttgttttctgccagtg-3') | 55 |
Human IL-6R | (5'-cagcagatgggctggcatgggaag/ccaagagcacagcctttgtc-3') | 55 |
Human OSM | (5'-gtaccgcgtgctccttgg/cggcctcgccatctgcagc-3') | 60 |
Human OSMR | (5'-gcaagtcaaggaaatgtcagtg/ccccaaggcagtgtccgtcc-3') | 60 |
Human gp130 | (5'-gaagcccaatccgccacataatttatc/cttcataggtgatcccacttg-3') | 60 |
Human GAPDH | (5'-gcgcctggtcaccagggctgc/gaatttgccatgggtggaatc-3') | 60 |