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Table 1 Primer pairs used for RT-PCR.

From: Oncostatin M promotes STAT3 activation, VEGF production, and invasion in osteosarcoma cell lines

Gene Primers (forward/reverse) Annealing Temp (°C)
Canine IL-6 (5'-gagattccaaggatgatg/gcctctttgctgtcttcaca-3') 55
Canine IL-6R (5'-gagatctgtgcagctcagcgactc/ctccactcacagaatacc-3') 55
Canine OSM (5'-gtccttggactcctgttcctg/cactcagcatctccaagtc-3') 55
Canine OSMR (5'-gtaaggtggaccgaggagatg/gagcaagccagtgtctctc-3') 55
Canine gp130 (5'-gaagcccagtccacc/ccttcatacaacgaatcc-3') 55
Canine GAPDH (5'-accacagtccatgccatcac/tccaccaccctgttgctgta-3') 55
Human IL-6 (5'-gctgctcctggtgttgcctg/ggttgttttctgccagtg-3') 55
Human IL-6R (5'-cagcagatgggctggcatgggaag/ccaagagcacagcctttgtc-3') 55
Human OSM (5'-gtaccgcgtgctccttgg/cggcctcgccatctgcagc-3') 60
Human OSMR (5'-gcaagtcaaggaaatgtcagtg/ccccaaggcagtgtccgtcc-3') 60
Human gp130 (5'-gaagcccaatccgccacataatttatc/cttcataggtgatcccacttg-3') 60
Human GAPDH (5'-gcgcctggtcaccagggctgc/gaatttgccatgggtggaatc-3') 60