qRT-PCR analysis of the Hedgehog pathway. Fold change from qRT-PCR of eight genes in the hedgehog pathway. Genes were selected for analysis based upon significance of the hedgehog pathway in pathway analysis. Fold change calculated via the 2(-ΔΔCt) method (* = p < 0.05). Error bars represent standard deviation and are asymmetrical due to the exponential fold change calculations. Inset: expression represented as 2(-ΔCt) for hedgehog ligands, error bars represent standard error.