Modulation of mdm2 pre-mRNA splicing by 9-aminoacridine-PNA (peptide nucleic acid) conjugates targeting intron-exon junctions

Table 2 Nomenclatures of primer sets and sequences of RT-PCR primers.

Primer set^(a),	Primer name	sequence (5'→3')	Product size (bp)	Remarks
A	Exo2S	CGATTGGAGGGTAGACCTGT	161	intron2 5'-splice site
	Int2AS	CACGATGAAAACTGGAAATCA
B	Int2S^a	TGCTTGTAGCTTTAGTTTTA	90	intron2
	Int2AS	CACGATGAAAACTGGAAATCA
C	Int2S^b	GATTTCCAGTTTTCATCGTGT	120	intron2 3'-splice site
	Exo3AS	GGTCTCTTGTTCCGAAGCTG
D	Exo3S	CAGCTTCGGAACAAGAGACC	169	intron3 5'-splice site
	Int3AS	GCAGTTACGCCAGAGGTAGC
E	Int3S	TCTTTGCTCTTTTGGATTGGA	251	intron3 3'-splice site
	Exo4AS	TTTTTGTGCACCAACAGACTTT
F	Exo4S	AAGCCATTGCTTTTGAAGTTATT	151	intron4 5'-splice site
	Int4AS	GCCAATTTCTCCACATGGTC
G	Int4S	TGGTTCCTGGTTGTTTACCC	215	intron5 3'-splice site
	Exo5AS	CACGCCAAACAAATCTCCTA
H	ActF1	CCCTGGAGAAGAGCTACGAG	223	β-actin (771-993)
	ActR1	ATGCCAGGGTACATGGTGGT
I	ActF2	CTTCCTGGGCATGGAGTC	166	β-actin (862-1027)
	ActR2	CAGGGCAGTGATCTCCTTCT

^(a)A-H indicates the target sequences for RT-PCR within the mdm2 pre-mRNA. For primer set H and I, location of the amplification products within the β-actin (Accsession#, BC001301) are indicated.

ISSN: 1471-2407