Figure 1

Design of siRNA targeting different encoding regions of heparanase. (A) Taken the heparanase mRNA (GenBank accession No. AF165154) as a target, three siRNA duplexes were designed and named as siH1, siH2, and siH3. Their corresponding targets located at 1088-1106 bp, 1267-1285 bp, and 1496-1514 bp of the encoding regions of heparanase mRNA. (B) Prediction of the secondary structures of siRNA targets was performed via RNAstructure 3.7 software. The siH1 recognized a region with three loops and two stems, while siH2 recognized a region with one loop and two stems. The target region of siH3 was sequestered intentionally in a stable stem structure. The red line indicated the target region of siRNA.