Table 1 Primer sequences and reaction conditions

GAPDH L

5' - CGACCACTTTGTCAAGCTCA - 3'

56°C

205 bp

GAPDH R

5'- AGGGGTCTACATGGCAACTG - 3'

56°C

ASPM L

5' - CAGTGATGTCACAGGGTTGG - 3'

54°C

272 bp

ASPM R

5' - CTCGTGAAAAAGCCAAAAGG - 3'

54°C

CENPF L

5' - GCCCATATATCCTGCGAAGA - 3'

56°C

258 bp

CENPF R

5' - GGCTGTCAGTCGGACTTCTC - 3'

56°C

NUSAP1 L

5' - TGTGCTTGGGACACACAAAT - 3'

54°C

297 bp

NUSAP1 R

5' - CGTTTCTTCCGTTGCTCTTC - 3'

54°C

PRC1 L

5' - ACAAAGGCTTCTAGGCGTGA - 3'

56°C

290 bp

PRC1 R

5' - GTGGCCACAGCTTCTCTTTC - 3'

56°C