Figure 8
Similarities of gene expression profiles in SW480 cells treated with PIAs and PKC signaling pathway inhibitors. (A) and (C) Each of the barviews is composed of 6100 individual treatment instances from the Connectivity Map (build 02) database ranked according to their similarity to the SH-5 or SH-6 gene signatures. The green area illustrate a positive, the red a negative and the gray field no enrichment with the indicated signatures. Black lines corresponding to distinct individual instances of MCF7 cells treated with either Resveratrol (A) or Rottlerin (C). Please note, that all marked instances are among the top ranking in SW480 cells, whereas they are broadly distributed all over the barviews in the other cell lines. (B) and (D) The enrichment of PIA specific gene signatures is demonstrated for the best matched individual treatment instances. Gene probes which are induced (green) or repressed (red) by PIAs were ordered according to their differential expression in MCF7 cells caused by the treatment with Resveratrol and Rottlerin, respectively (x-axis). The running sum of the Kolomogorov-Smirnov test is shown at the y-axis. Most genes which are induced by the PIAs appear early in the treatment profile, whereas the converse is true for repressed genes. (E) The treatment of SW480 cells with either Resveratrol or Rottlerin for 48 hours results in binucleated cells as detected by DAPI staining.