Examination of gene expression profile using MYC-ER inducible system in RWPE1 cells and its validation of selected genes in LNCaP and DU145 cells. (A) A MYC-ER inducible system was established in RWPE1 prostate cells and shown is western blot analysis of stable protein expression of MYC-ER, Pim1 and Actin (arrows) in RWPE1 cell lines. * marks a non-specific band. (B) RT-PCR confirmed mRNA expression of several MYC/Pim1 target genes selected from Table 1 in LNCaP-Pim1 and DU145-Pim1 cells. Arrows indicate selected genes that are consistently regulated by Pim1 in DU145 and LNCaP cells.