Pim1 enhances tumorigenicity of androgen-dependent human prostate cancer cells (LNCaP) in vitro and in vivo. (A) Soft agar assay showed increased in vitro tumorigenicity of Pim1-expressing LNCaP cells. When control or Pim1-expressing LNCaP cells were subcutaneously grafted in nude mice, the latter developed larger tumors in size (B) and weight (C). (D) Kaplan-Meier survival analysis demonstrates slightly accelerated tumor onset by Pim1 expression. Numbers in the parentheses indicate the number of replicates or grafts in each group. (E) H&E stains demonstrated that Pim1 expression caused more hemorrhagic phenotype than control. *P < 0.05.