Figure 5

Effect of CARM1 in p53 and NF-κB mediated signals. LNCaP cells were transiently transfected with 100 ng of p53-luc (A, C), p21-luc (B), or NF-κB-luc (D, E) and 100 ng of pSG-CARM1 and 2 ng of renilla. In C-E, pRC/RSV-p300 was cotransfected. To activate the NF-κB response, TNF-α was added at a final concentration of 10 ng/ml after transfection; E, pSG-CARM1 (S229E) mutants were transfected up to 100 ng. F, E2F1-luc, TCF4RE-luc, and cmyc-luc were tested for the CARM1 response. Luciferase assays were performed 48 h post-transfection; each bar represents the mean ± S.D. *, p < 0.01.