A rapid, sensitive, reproducible and cost-effective method for mutation profiling of colon cancer and metastatic lymph nodes

Table 6 Allele frequencies for primary tumors with two mutations and metastatic lymph nodes

Sample	Mutations	Mutant Allele Frequency		Mutation Ratios
		Primary	Lymph Node	Primary M1/M2	Lymph node M1/M2
C07-0388	M1. KRAS-G12D	0.45	0.44	1.88	1.63
	M2. PIK3CA-H1047R	0.24	0.27
C07-0717	M1. KRAS-G13D	0.08	0.09	1.14	0.90
	M2. PIK3CA-H1047R	0.07	0.1
C07-0940	M1. KRAS-G12D	0.42	0.34	1.91	1.10
	M2. PIK3CA-E542K	0.22	0.31
C07-2244	M1. KRAS-G12C	0.37	0.15	1.76	_
	M2. PIK3CA-H1047R	0.21	0
C07-1837	M1. BRAF-V600E	0.2	0.22	1.67	4.40
	M2. PIK3CA-H1047R	0.12	0.05

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