Fig. 3

HHT induced apoptosis by p38 MAPK/H2AX/Mcl-1 axis. A, B, D HL-60 and THP-1 cells were pretreated with 10 μM SP600125, a p38 inhibitor (10 μM SB203580) or PH797804, an ERK inhibitor (10 μM PD98059), for 4 h and then treated with increasing concentrations of HHT for another 24 h, after which apoptosis was detected by AO-EB staining. C, E, Protein expression in HL-60 or THP-1 cells treated with SB203580 and HHT for 24 h. F, G, p38 was silenced with p38 siRNA in THP-1 cells that were subsequently treated with HHT. *, P < 0.05; **, P < 0.01; ***, P < 0.001