Fig. 2

HHT induced apoptosis, decreased the protein levels of Mcl-1, and increased the phosphorylation of p38 and H2AX in AML cells. A, E, The cell inhibition and apoptosis percentages of HL-60 or THP-1 cells treated with increasing concentrations of HHT for 24 h were examined by trypan blue and AO-EB staining. B, F, The percentage of HL-60 or THP-1 cells in which apoptosis was induced, respectively, after treatment with increasing concentrations of HHT for 12 h or 24 h was examined by AO-EB staining. C, G, Western blot analysis of the protein expression of PARP, caspase-3, cleaved caspase-3 (C-C3), MAPK kinase, Mcl-1 and p-H2AX in HL-60 and THP-1 cells treated with increasing concentrations of HHT for 24 h. D, H, Protein expression in cells treated with HHT for 1, 3, 6, 12 or 24 h