Fig. 6

NRAV induces iron export and ferroptosis resistance via sponging miR-375-3P in HCC. A Iron content increased after NRAV knockdown and decreased after NRAV overexpression. B-C Flow cytometry of ROS levels in HCC cells after NRAV knockdown or overexpression. D Western blot showed expressions of the ferroptosis-related proteins (ACSL4, SCL7A11, and GPX4) in hepG2 Cell with NRAV knockdown and HuH-7 Cell with NRAV overexpression and control. E NRAV and miR-375-3P binding to the secondary structural diagram from AnnoLnc. F RIP assay confirmed the interaction of miR-375-3P with NRAV in HCC cells. G Schematic illustration of the NRAV-WT and NRAV-Mut luciferase vectors. H Relative luciferase activities in hepG2 and HuH-7 cells co-transfected with NRAV-WT or NRAV-Mut and the miR-375-3P mimic, inhibitor, or corresponding negative control. I-J The expression level of miR-375-3P in each group transfected was verified through RT-qPCR K. The SLC7A11 protein levels in hepG2 and HuH-7 cells from different groups were determined by western blot analysis. GAPDH was used as a control. Results are presented as mean ± SD. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001