Fig. 9
Analysis of the expression characteristics of the five differentially expressed genes (DEGs) in hepatocellular carcinoma (HCC) tissues. (A and B) Immunohistochemistry (IHC) analysis was performed to detect the expression of CYP26B1, CDK5R2, PAGE1, TRIM36, DCAF8L1, and m1A in HCC tissues (n = 90) and para-carcinoma tissues (n = 90; magnifications: 100× and 200×); (C) Heatmaps of correlations demonstrating the spectrum of relationships among targeting m1A, CYP26B1, CDK5R2, PAGE1, TRIM36, and DCAF8L1. The bar ranging from blue to red (− 1 to 1) represents negative to positive correlations, respectively; (D) Heatmaps of correlations demonstrating the spectrum of relationships among targeting CYP26B1, CDK5R2, PAGE1, TRIM36, DCAF8L1, m1A, and clinical features. The bar ranging from blue to red (− 1 to 1) represents negative to positive correlations, respectively; (E) Expression of TRIM36, CYP26B1, PAGE1, CDK5R2, and DCAF8L1 in sh-ALKBH3 and shNC HepG2 cells assessed through western blot (left) and quantitatively analyzed (right); (F) HepG2 cells were transfected with vector control, ALKBH3, ALKBH3-R122S, or ALKBH3-L177A constructs for 48 h, the expression of TRIM36, CYP26B1, PAGE1, CDK5R2, and DCAF8L1 were checked by western blot (left) and quantitatively analyzed (right); *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant