Fig. 3

High m¹A modification inhibits HCC cell proliferation and migration. (A) ALKBH3 expression detected by western blotting (up) and quantitatively analyzed (down); (B) Levels of mRNA m¹A modification in sh-ALKBH3 HepG2, sh-ALKBH3 QGY, and their corresponding control cells assessed through dot blot analysis; (C) Relative cell viability of sh-ALKBH3 HepG2, sh-ALKBH3 QGY, and their corresponding control cells; (D and E) Results of colony formation (D) and migration (E) assays to detect the effects of m¹A modification on HepG2 and QGY cells; (F and G) Levels of mRNA m¹A modification (F) and relative cell viability (G) of HepG2 cells transfected with vector control (PPB), ALKBH3, ALKBH3-R122S, or ALKBH3-L177A constructs for 24 h (H) Results of colony formation of HepG2 cells transfected with vector control (PPB), ALKBH3, ALKBH3-R122S, or ALKBH3-L177A. Data are presented as mean ± standard deviation from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group