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Fig. 7 | BMC Cancer

Fig. 7

From: Cellular senescence and metabolic reprogramming model based on bulk/single-cell RNA sequencing reveals PTGER4 as a therapeutic target for ccRCC

Fig. 7

PTGER4 repressed cell senescence and lipid accumulation in RCC cells. (A) Gene set enrichment analysis (GSEA) showed that the differential analysis of high or low PTGER4 subgroups was mainly enriched in metabolic-related pathways. (B) Protein expression levels of PTGER4 in normal renal cells and renal cancer cells. (C) A CCK-8 assay was performed to detect RCC cell proliferation. NC is a blank vector control overexpressing PTGER4. The chart shows the mean ± SEM (n = 3 for each group). (D) EdU showed the proliferation ability of Caki-1 and OS-RC-2 cells after overexpression of PTGER4. There was no paired t test. (E) The proliferation ability of Caki-1 and OS-RC-2 cells after PTGER4 overexpression is shown. No paired t test, analysis of variance. (F) BODIPY staining showed the content of neutral fatty acids in Caki-1 and OS-RC-2 cells after PTGER4 overexpression. No paired T Test. (G) The content of CHO, FFAs and TG in Caki-1 and OS-RC-2 cells after PTGER4 overexpression (H) showed that the content of β-galactosidase in Caki-1 and OS-RC-2 cells after PTGER4 overexpression (I) showed cell cycle changes after PTGER4 overexpression. (J) Changes in the cell cycle and senescence-related protein expression after PTGER4 overexpression

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BMC Cancer

ISSN: 1471-2407

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