Fig. 6

TNFRSF17 silencing inhibited 25-HC-induced cell migration and invasion. A549 and SPC-A1 cells were transfected with the small interfering RNAs carrying siNC or siTNFRSF17, and were then exposed to 0, 0.013 and 0.085 µM 25-HC for 48 h. Cell migration was performed by transwell assay (A). Cell invasion was performed by transwell assay (coating with Matrigel) (B).Data were shown as mean ± SD of at least three independent experiments.*, p < 0.05; **, p < 0.01;*** p < 0.0001